LOCUS       pEX-N-His.        4539 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) bacterial vector for inducible expression of a 
            protein with a cleavable N-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pEX-N-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..4539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        211..229
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    230..254
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             258..280
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             288..290
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             291..308
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             309..329
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    330..416
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      455..501
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        747..824
                     /label=lacI promoter
     CDS             825..1904
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1920..1941
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1956..1986
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2018..2034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2050..2066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2358..2946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3120..3977)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3978..4082)
                     /label=AmpR promoter
     rep_origin      4109..4539
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcga
      181 attctgctgg actttccaaa atgtcgagct taatacgact cactataggg gaattgtgag
      241 cggataacaa ttcccctttt gtttaacttt aagaaggaga ggccaccatg catcaccatc
      301 accatcacga aaacctgtat tttcagggcg cgatcgccgg cgcgccagat ctcaagctta
      361 actagctagc ggaccgacgc gttaaacgcg gccgctcgag gtttaaacgg ccggccacat
      421 gactgactga gccacgataa ccgctgagca ataactagca taaccccttg gggcctctaa
      481 acgggtcttg aggggttttt tcccaccagc cttgtcctaa tccgcggcgg ttcctggcct
      541 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      661 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
      721 gcggtatttc acaccgcata aattccgaca ccatcgaatg gcgcaaaacc tttcgcggta
      781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
      841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgagt cagtgggctg atcattaact
     1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     1621 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1921 aatgtaagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1981 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2041 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2101 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2161 caccgatgga tccgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcc
     2581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2761 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4081 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     4141 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4201 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4261 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4321 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4381 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4441 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4501 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
