LOCUS       40924_18681        2450 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Streamlined bacterial cloning vector with an ampicillin resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Eurofins Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(41..57)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(65..95)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(110..131)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(419..1007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1181..2038)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2039..2143)
                     /label=AmpR promoter
ORIGIN
        1 acctgcggcc gcaagcttgg atccgaattc ctgtgtgaaa ttgttatccg ctcacaattc
       61 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      841 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1141 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1201 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1261 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1321 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1381 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1441 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1501 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1561 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1621 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1681 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1741 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1801 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1861 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1921 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1981 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2041 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2101 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2161 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2221 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     2281 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     2341 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     2401 agattgtact gagagtgcac catatgggta ccgagctcgc ggccgcaagc
//
