LOCUS       V015477                 5314 bp    DNA     circular SYN 13-FEB-2019
DEFINITION  Exported.
ACCESSION   V015477
VERSION     V015477
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Stoudenmire JL, Fidopiastis PM, Black M, Stabb EV.
  TITLE     Mutagenesis of Vibrio fischeri and other marine bacteria using
            hyperactive mini-Tn5 derivatives
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Stoudenmire JL, Fidopiastis PM, Black M, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30602, USA"
FEATURES             Location/Qualifiers
     source          1..5314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..19
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     regulatory      complement(337..361)
                     /regulatory_class="terminator"
     CDS             complement(507..1241)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     regulatory      complement(1249..1256)
                     /regulatory_class="ribosome_binding_site"
     rep_origin      1560..1948
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1968..1992
                     /regulatory_class="terminator"
     primer_bind     2076..2092
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2107..2125)
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             2259..3071
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(3418..3527)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3802..5229)
                     /label="Tn5 transposase"
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
ORIGIN
        1 ctgtctctta tacacatcta aggtaatcag gagctaagga agctaaaatg gagaaaaaaa
       61 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      121 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      181 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      241 gcctgatgaa tgctcatccg ggtacaactg aggatccggt gattgattga gcaagcttta
      301 tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc
      361 caattactag ccaattcgat atcaagctta tcgctaggga cctctttagc tccttggaag
      421 ctgtcagtag tatacctaat aatttatcta cattcccttt agtaacgtgt aactttccaa
      481 atttacaaaa gcgactcata gaattatttc ctcccgttaa ataatagata actattaaaa
      541 atagacaata cttgctcata agtaacggta cttaaattgt ttactttggc gtgtttcatt
      601 gcttgatgaa actgattttt agtaaacagt tgacgatatt ctcgattgac ccattttgaa
      661 acaaagtacg tatatagctt ccaatattta tctggaacat ctgtggtatg gcgggtaagt
      721 tttattaaga cactgtttac ttttggttta ggatgaaagc attccgctgg cagcttaagc
      781 aattgctgaa tcgagacttg agtgtgcaag agcaacccta gtgttcggtg aatatccaag
      841 gtacgcttgt agaatccttc ttcaacaatc agatagatgt cagacgcatg gctttcaaaa
      901 accacttttt taataatttg tgtgcttaaa tggtaaggaa tactcccaac aattttatac
      961 ctctgtttgt tagggaattg aaactgtaga atatcttggt gaattaaagt gacacgagta
     1021 ttcagtttta atttttctga cgataagttg aatagatgac tgtctaattc aatagacgtt
     1081 acctgtttac ttattttagc cagtttcgtc gttaaatgcc ctttacctgt tccaatttcg
     1141 taaacggtat cggtttcttt taaattcaat tgttttatta tttggttgag tactttttca
     1201 ctcgttaaaa agttttgaga atattttata tttttgttca tgtaatcact ccttcttaat
     1261 tacaaatttt tagcatctaa tttaacttca attcctatta tacaaaattt taagatactg
     1321 cactatcaac acactcttaa gtttgcttct aagtcttatt tccataactt cttttacgtt
     1381 tccgccattc tttgctgttt cgatttttat gatatggtgc aagtcagcac gaacacgaac
     1441 cgtcttatct cccattatat ctttttttgc actgattggt gtatcatttc gtttttcttt
     1501 ttctgcgata ccgtcgagat atcctgcaga gatccggcca cgatgcgtcc ggcgtagagg
     1561 atctgaagat cagcagttca acctgttgat agtacgtact aagctctcat gtttcacgta
     1621 ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct
     1681 aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc
     1741 atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt
     1801 tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta acggttgtgg
     1861 acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga
     1921 gtgacacagg aacacttaac ggctgacatg ggaattggct agtaattggg gaccctagag
     1981 gtcccctttt ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc
     2041 aatcaatcac cggatccact agttctaggg gccctgtaaa acgacggcca gtcctagagc
     2101 ggccgcagat gtgtataaga gacaggtcga cctgcagggg ggggggggaa agccacgttg
     2161 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     2221 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     2281 cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2341 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
     2401 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2461 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2521 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc
     2581 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2641 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2701 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2761 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
     2821 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2881 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2941 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3001 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3061 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3121 cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     3181 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     3241 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     3301 tggggcgatt caggcttaat tctggaaggc agtacacctt gataggtggg ctgcccttcc
     3361 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg
     3421 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     3481 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     3541 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     3601 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
     3661 agcgctgctt ccctgctgtt ttgtggaata tctaccgact ggaaacaggc aaatgcagga
     3721 attaagcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgcccccccc
     3781 cccctgcagg tcgacggatc agatcttgat cccctgcgcc atcagatcct tggcggcaag
     3841 aaagccatcc agtttacttt gcagggcttc ccaaccttcc cagagggcgc cccagctggc
     3901 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     3961 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     4021 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     4081 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctttctc tgagctgtaa
     4141 cagcctgacc gcaacaaacg agaggatcga gaccatccgc tccagattat ccggctcctc
     4201 catgcgttgc ctctcggctc ctgctccggt tttccatgcc ttatggaact cctcgatccg
     4261 ccagcgatgg gtataaatgt cgatgacgcg caaggcttgg gctagcgact cgaccggttc
     4321 gctggtcagc aacaaccatt tcaacggggt ctcacccttg ggcgggttaa tctcctcggc
     4381 cagcaccgcg ttgagcgtga tattcccctg ttttagcgtg atgcgcccac tgcgcaggct
     4441 caagctcgcc ttgcgggctg gtcgattttt acgtttaccg cgtttatcca ccacgccctt
     4501 ttgcggaatg ctgatctgat agccacccaa ctccggttgg ttcttcagat ggtcgtacag
     4561 atacaaccca gactctacgt ccttgcgtgg gtgcttggag cgcaccacga agcgctcgtt
     4621 atgcgccagt ttgtcctgca gataagcatg aatatcggct tcgcggtcac agaccgcaat
     4681 cacgttgctc atcatgctgc ccatgcgtaa ccggctagtt gcggccgctg ccagccattt
     4741 gccactctcc ttttcatccg catcggcagg gtcatccggg cgcatccacc actcctgatg
     4801 cagtaatcct acggtgcgga atgtggtggc ctcgagcaag agaacggagt gaacccacca
     4861 tccgcgggat ttatcctgaa tagagcccag cttgccaagc tcttcggcga cctggtggcg
     4921 ataactcaaa gaggtggtgt cctcaatggc cagcagttcg ggaaactcct gagccaactt
     4981 gactgtttgc atggcgccag cctttctgat cgcctcggca gaaacgttgg gattgcggat
     5041 aaatcggtaa gcgccttcct gggcggcttt actaccctct gatgagatgg ttattgattt
     5101 accagaatat tttgccaatt gggcggcgac gttaaccaag cgggcagtac ggcgaggatc
     5161 acccagcgcc gccgaagaga acacagattt agcccagtcg gccgcacgat gaagagcaga
     5221 agttatcatg aacgttacca tcttaagagg tcacctggaa gatcagatcc tggaaaacgg
     5281 gaaaggttcc gttcaggacg ctacggatct ggat
//
