LOCUS       V006449                 4892 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006449
VERSION     V006449
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 4892)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4892)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
            Meadow Circle, Palo Alto, CA 94303, USA"
            SGRef: number: 3; type: "Journal Article"
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International
            customers, please contact your local distributor.  For technical
            information, call (415) 424- 8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence
            databases, published literature and other sources, together with
            partial sequences obtained by CLONTECH; this vector has not been
            completely sequenced. If you suspect there is an error in this
            sequence, please contact CLONTECH's Technical Service Department at
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..4892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             335..520
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     polyA_signal    complement(1531..1665)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2537..3125)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3299..4156)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4157..4261)
                     /label="AmpR promoter"
     promoter        complement(join(4826..4892,1..291))
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
      181 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      241 tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
      301 aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
      361 ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
      421 tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
      481 acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
      541 tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
      601 actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
      661 gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
      721 tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
      781 cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
      841 gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
      901 tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
      961 tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
     1021 ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
     1081 aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
     1141 aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
     1201 ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
     1261 aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
     1321 agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
     1381 ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
     1441 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaccggatc
     1501 gatccctcga gtctagaccc ggggagctcg gatccagaca tgataagata cattgatgag
     1561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     1741 ctctacaaat gtggtatggc tgattatgat cctctacgcc ggacgcatcg tggccggcat
     1801 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     1861 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     1921 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     1981 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     2041 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2161 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     2221 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     2281 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     2341 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     2401 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     2461 agatgacgac catcagggac agcttcaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2941 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3601 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3901 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4321 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca tgtttgacag
     4381 cttatcatcg aagcagtagc aatcaaccca cacaagtgga tctttcctgt ataattttct
     4441 attttcatgc ttcatcctca gtaagcacag caagcatatg cagttagcag acattttctt
     4501 tgcacactca ggccattgtt tgcagtacat tgcatcaaca ccaggattta aggaagaagc
     4561 aaatacctca gttgcatccc agaagcctcc aaagtcaggt tgatgagcat attttactcc
     4621 atcttccatt ttcttgtaca gagtattcat tttcttcatt ttttcttcat ctcctccttt
     4681 atcaggatga aactccttgc atttttttaa atatgccttt ctcatcagag gaatattccc
     4741 ccaggcactc ctttcaagac ctagaaggtc cattagctgc aaagattcct ctctgtttaa
     4801 aactttatcc atctttgcaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     4861 actacttctg gaatagctca gaggccgagg cg
//
