LOCUS       pETite_C-His_Kan        2235 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for Expresso(R) cloning to add a C-terminal 6xHis tag, with 
            inducible expression from the T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pETite C-His Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..42
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..86
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..145
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             146..163
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      195..242
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      340..369
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      391..978
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(990..1017)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1044..1232)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(1236..2048)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2049..2139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2140..2171)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
ORIGIN
        1 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc taatacgact
       61 cactataggg tgtgagcgga taacaatttc acgtggaaca gctagaaata attttgttta
      121 actataagaa ggagatatac atatgcatca tcaccaccat cactaataga gcggccgcca
      181 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      241 tgctgaaagg aggaactata tccgggtaac gaattcaagc ttgatatcat tcaggacgag
      301 cctcagactc cagcgtaact ggactgcaat caactcactg gctcaccttc acgggtgggc
      361 ctttcttcgg tagaaaatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      481 accaactctt tttccgaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      901 agtcctgtcg ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg
      961 gggcggagcc tatggaaaaa cgccagcaac gcagaaaggc ccacccgaag gtgagccagg
     1021 tgattacatt tgggccctca tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     1081 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     1141 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     1201 atgttaaggg cggttttttc ctgtttggtc atttagaaaa actcatcgag catcaagtga
     1261 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     1321 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     1381 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     1441 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     1501 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     1561 gcaccaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     1621 ccgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     1681 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     1741 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     1801 gtcggaagag gcataaattc cgtcagccag tttagcctga ccatctcatc tgtaacatca
     1861 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     1921 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     1981 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     2041 tggctcatag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2101 tcattatggt gaaagttgga acctcttacg tgccgatcaa gtcaaaagcc tccggtcgga
     2161 ggcttttgac tttctgctat ggaggtcagg tatgatttaa atggtcagta ttgagcgata
     2221 tctagagaat tcgtc
//
