LOCUS       V015819                 4009 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015819
VERSION     V015819
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..4009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1492
                     /gene="glpK"
                     /label="Glycerol kinase"
                     /note="Glycerol kinase from Thermococcus kodakarensis
                     (strain ATCC BAA-918 / JCM 12380 / KOD1). Accession#:
                     O93623"
     terminator      1514..1561
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(1927..1955)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        2068..2172
                     /label="AmpR promoter"
     CDS             2173..3030
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3204..3792
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3872..3890
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3922..3944
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3963..3980
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3990..4007
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 tatggaaaaa tttgtgctga gcctggatga aggtacaacc agcgcacgtg caattatctt
       61 tgatcgtgaa agcaacattc atggcattgg ccagtatgaa tttccgcagc attatccgcg
      121 tccgggttgg gttgaacata atccggaaga aatttgggat gcacagctgc gtgccattaa
      181 agatgcaatt cagagcgcac gtattgaacc gaatcagatt gcagcaattg gtgttaccaa
      241 tcagcgtgaa accaccctgg tttgggataa agatggtaaa ccgctgtata atgcaattgt
      301 ttggcagtgt cgtcgtaccg cagaaatggt tgaagaaatt aaacgtgaat acggcaccat
      361 gatcaaagaa aaaaccggtc tggttccgga tgcatatttt agcgcaagca aactgaaatg
      421 gctgctggat aatgttccgg gtctgcgtga aaaagccgaa aaaggtgaag ttatgtttgg
      481 caccgttgat acctttctga tttatcgtct gaccggtgaa catgtgaccg attatagcaa
      541 tgcaagccgt accatgctgt ttaacatcaa aaaactggat tgggatgatg agctgctgga
      601 actgtttgat attccggaaa gcgttctgcc ggaagttcgt gaaagtagcg aagtttatgg
      661 ctataccaaa aaagaactgc tgggtgcaga aattccggtt agcggtgatg ccggtgatca
      721 gcaggcagca ctgtttggtc aggcagcatt tgaagcaggt atggttaaag caacctatgg
      781 caccggtagc tttattctgg ttaataccga taaaatggtc ctgtatagcg ataatctgct
      841 gaccaccatt gcatggggtc tgaatggtcg tgttagctat gcactggaag gtagcatttt
      901 tgttaccggt gcagcagttc agtggctgcg tgatggcatt aaaatcatta aacatgccag
      961 cgaaaccgaa gaactggcaa ccaaactgga aagcaatgaa ggtgtttatt ttgtgcctgc
     1021 atttgttggt ctgggtgcac cgtattggga tcagtttgca cgtggtatta tcattggtat
     1081 tacccgtggt acaggtcgtg aacatctggc acgtgcgacc ctggaagcaa ttgcatatct
     1141 gacccgtgat gttgttgatg agatggaaaa actggtgcag attaaagaac tgcgtgttga
     1201 tggtggtgca accgcaaatg attttctgat gcagtttcag gccgatattc tgaatcgtaa
     1261 agttattcgt ccggtggtga aagaaaccac cgcactgggt gccgcatatc tggcaggtct
     1321 ggcagttgat tattgggcag atacccgtga aattgcagaa ctgtggaaag cagaacgtat
     1381 ttttgagccg aaaatggatg aaaaaacccg tgaacgtctg tacaaaggtt ggaaagaagc
     1441 agttaaacgc gcaatgggtt gggcaaaagt tgttgatagc gcaaaaagca actgaggatc
     1501 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     1561 gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt
     1621 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg
     1681 acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca
     1741 cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta
     1801 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
     1861 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
     1921 taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
     1981 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2041 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2161 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2221 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2281 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2341 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2401 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2461 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2521 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2581 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2641 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2701 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2761 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2821 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2881 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2941 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3001 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3061 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3841 tttgctcaca tggatctcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa
     3901 cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
     3961 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagcca
//
