LOCUS       V006455                 5246 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006455
VERSION     V006455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Thermostabilization of luciferase by in vivo directed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 5246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2010) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..1750
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     CDS             1763..1780
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1847..1894
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1931..2386
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2413..2517
                     /label="AmpR promoter"
     CDS             2518..3375
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3549..4137
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4323..4465)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4570..4758)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacat atggctagca aaatggaaat
      121 ggaaaaggag gagaatgttg tatatggccc tctgccattc taccccattg aagaaggatc
      181 agctggaatt cagttgcata agtacatgca tcaatatgcc aaacttggag caattgcttt
      241 ttcgaacgcc cttactggag tcgacatttc ttaccaagaa tactttgata ttacatgtcg
      301 tttagctgag gccatgaaaa actttggtat gaaaccggaa gaacatattg ctttgtgcag
      361 tgaaaattgt gaagaatttt tcatccctgt acttgctggt ctttacattg gggtagctgt
      421 tgcacctact aatgaaattt acacattgcg tgaacttaat catagtttgg gcatcgcaca
      481 accaactatt gtattcagct cgagaaaagg cttacctaaa gttttagaag tgcaaaaaac
      541 agttacatgc atcaaaaaaa ttgttatttt agatagtaaa gtaaactttg ggggccacga
      601 ttgtatggaa acttttatta agaaacatgt agaattaggt tttcaaccaa gtagctttgt
      661 acccattgat gtaaagaacc gtaaacaaca cgttgctttg cttatgaatt cttctggctc
      721 tactggttta cctaaaggtg tacgaattac ccacgaaggt gcagttacaa gattctcaca
      781 cgctaaggat ccaatttacg gaaaccaagt ttcacctggt actgctattt taactgtcgt
      841 tccgttccat catggatttg gaatgtttac cactttagga tactttgctt gtggataccg
      901 tgttgtaatg ttaacaaaat ttgatgaaga actatttttg agaactttgc aagattataa
      961 gtgtaccagt gttattctgg taccaacgtt atttgctatt ctcaacaaga gtgaattgat
     1021 cgataagttc gatttatcta atctaactga aattgcttct ggtggagctc ctttggcaaa
     1081 agaagttggc gaagcagtcg ctagaagatt taatctaccc ggtgtccgtc agggttacgg
     1141 attaacagaa acgacatctg catttattat taccccagaa ggtgatgata aacctggagc
     1201 atctggaaaa gtagtaccct tattcaaagt aaaagttatt gatcttgaca ctaaaaaaac
     1261 tttgggtgtc aaccgacgag gagagatctg tgtaaaagga cccagtctta tgttaggcta
     1321 ctcgaacaat ccggaagcaa caagagaaac tattgatgaa gagggttggt tgcacacagg
     1381 agatattgga tattacgacg aagacgaaca tttcttcatt gtcgatcgtt tgaaatcatt
     1441 aatcaaatac aaggggtacc aggtaccacc tgctgaattg gaatccgttc ttttgcaaca
     1501 tccaaatata tttgatgctg gtgtggctgg tgtccccgat cccgatgctg gcgaacttcc
     1561 aggggctgta gttgtaatgg aaaaaggaaa aactatgact gaaaaggaaa ttgtggatta
     1621 tgttaatagt caagtagtga accacaaacg tctgcgtggt ggcgttcgtt ttgtggatga
     1681 agtacctaaa ggtctaactg gtaaaattga tgctaaagta attagagaaa ttcttaagaa
     1741 accacaatcg ggcccagtcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
     1801 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     1861 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
     1921 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1981 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2041 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2101 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2161 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2221 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2281 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2341 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2761 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3061 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3661 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4321 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt
     4381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     4441 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     4501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     4621 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     4681 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     4741 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     4801 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     4861 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     4921 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     4981 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     5041 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     5101 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     5161 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     5221 ccgtggccag gacccaacgc tgcccg
//
