LOCUS       V013949                 9057 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013949
VERSION     V013949
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2265..4004
                     /gene="mcjD"
                     /label="Microcin-J25 export ATP-binding/permease protein
                     McjD"
                     /note="Microcin-J25 export ATP-binding/permease protein
                     McjD from Escherichia coli. Accession#: Q9X2W0"
     promoter        4079..4097
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4098..4122
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4231..4275
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      4327..4374
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      4411..4866
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(4984..5841)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5842..5934)
                     /label="AmpR promoter"
     rep_origin      6015..6603
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6789..6931)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(7036..7224)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(7762..7783)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7799..8878)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(8879..8956)
                     /label="lacI promoter"
ORIGIN
        1 ctctttttga tgcaatccgc tttgcttctg actataatag tcagggtaat tgagtgtaaa
       61 ggcataacta caggagggag tgtgcaaaat gatccgttac tgcttaacca gttatagaga
      121 ggatcttgtt atcctggata taattaatga tagtttcagc atagtgcctg acgcaggtag
      181 cttgctaaaa gaaagagata aattgcttaa agaattccca caactatctt acttttttga
      241 cagtgaatat catattggaa gtgtttctcg taatagtgac acttcttttc ttgaagaacg
      301 ctggtttcta ccagaacctg acaaaacatt atataagtgt tctctattta aacgatttat
      361 attattactc aaagtctttt actatagctg gaatattgaa aaaaaaggga tggcatggat
      421 tttcataagt aataaaaaag agaataggct atactccttg aatgaagagc atcttatccg
      481 gaaagaaatt agtaatcttt ccattatctt tcatcttaat atttttaaat ctgactgtct
      541 tacctattca tacgcactaa aaagaattct taattccaga aatattgatg ctcatcttgt
      601 tattggtgta aggacacaac ctttttatag ccactcttgg gtggaggttg ggggacaagt
      661 tatcaatgat gctcccaata tgcgggataa attatctgtt attgcagaga tatagttatg
      721 gaaatattta atgtcaagtt aaatgatact tcaattagaa ttattttctg taaaacgctt
      781 tctgccttcc ggacagaaaa taccatcgtt atgctcaaag gaaaagcagt ttcaaatggc
      841 aaacctgtat ccacagagga gattgccaga gtagtggaag aaaaaggtgt ttcagaagta
      901 atagaaaatt tagatggtgt tttctgtatc ctaatttatc attttaatga tctccttata
      961 gggaaaagca ttcaatcagg ccccgctcta ttttattgta aaaagaatat ggatattttt
     1021 gtttcggata aaatttctga tatcaaattt ttgaatccag atatgacatt cagtctaaat
     1081 ataacaatgg cagaacatta tctgtcagga aatcgaatag caacccagga atcactaatc
     1141 actggcattt acaaagtaaa taatggtgag tttataaaat ttaataatca gttgaaacct
     1201 gtgctacttc gtgatgagtt tagtattacc aaaaagaaca attcaactat cgacagtatc
     1261 attgataata ttgagatgat gcgggataat agaaaaatag ccctattatt ttccggagga
     1321 ttggattctg cattaatttt tcacacactt aaagaatcag gtaacaaatt ctgcgcttat
     1381 catttttttt ctgatgaatc tgatgacagt gaaaagtatt ttgctaagga atactgttca
     1441 aaatatggag ttgattttat atctgttaat aaaaacatca actttaatga aaaactttat
     1501 ttcaatttaa atcctaatag tccggacgaa atccctttga tatttgaaca gacagatgaa
     1561 gaaggtgaag gtcagccccc catagacgat gatttattat atctatgtgg tcacggtgga
     1621 gatcatattt tcggacaaaa tccttcagaa ctttttggca ttgatgcata tcgaagtcat
     1681 ggcttgatgt ttatgcataa aaaaatagta gaattttcca atctcaaggg aaagagatat
     1741 aaagatatca tattttcaaa tatttccgca ttcattaata catccaacgg atgttctcca
     1801 gcaaagcaag agcacgtatc agatatgaaa cttgcctctg ctcagttttt tgcaactgat
     1861 tatacaggaa aaattaataa actaactcca ttcctgcata aaaatattat ccagcattat
     1921 gctggcttac cagtttttag tctatttaac cagcactttg atcgttatcc cgttcgttat
     1981 gaagcgtttc aacgatttgg ttcagatatt ttctggaaaa aaaccaaacg gtcatcttca
     2041 tcatgactaa tattcagaat tctatccggt aaaaaggatg aactagtgaa tacaataaaa
     2101 cagtcaggat taattgaaat attaggcatt aaccatattg aattggaaag cattttgtat
     2161 gaaaatacga ctacacgtct gacaatggaa ctaccatata tacttaactt ataccgtctg
     2221 gcaaaattca ttcaacttca atccattgat tataaaggtt aattatggaa agaaaacaga
     2281 aaaactcatt atttaattat atttattcat taatggatgt aagaggtaaa tttttattct
     2341 tttccatgtt attcattaca tcattatcat cgataatcat atctatttca ccattgattc
     2401 ttgcaaagat tacagattta ctgtctggct cattgtcaaa ttttagttat gaatatctgg
     2461 ttttacttgc ctgtttatac atgttttgcg ttatatctaa taaagcaagt gtttttttat
     2521 ttatgatact gcaaagtagt ctacgtatta acatgcagaa aaaaatgtcg ctaaagtatt
     2581 tgagagaatt gtataacgaa aatataacta acttgagtaa aaataatgct ggatatacaa
     2641 cgcaaagtct taaccaggct tcaaatgaca tttatattct tgtgagaaat gtttcccaga
     2701 atatcctgtc acctgttata caacttattt ccactattgt tgttgtttta tctacgaagg
     2761 actggttttc tgccggtgtg ttttttctct atattctggt atttgtaatt tttaatacca
     2821 gactgactgg cagtttagcg tctctcagaa aacacagcat ggatatcact cttaactctt
     2881 atagtctgtt atctgatact gttgataaca tgatagcagc taaaaagaat aatgcattaa
     2941 gacttatttc tgaacgttat gaagatgctc tcactcagga aaacaatgct cagaaaaaat
     3001 actggttact cagttctaaa gttcttttat tgaactcttt acttgctgta atattatttg
     3061 gttctgtatt catatataat attttaggtg tgctgaatgg tgtagttagt atcggccact
     3121 tcattatgat tacatcatat atcattcttc tttcaacgcc agtggaaaat ataggggcat
     3181 tgctaagtga gatcaggcag tcaatgtcta gcctggcagg ttttattcaa cgtcatgccg
     3241 agaataaagc cacatctcct tcaatacctt ttctcaacat ggagcgaaaa ttaaacctgt
     3301 ccataagaga gctttcattt agctatagtg atgataaaaa aatacttaat tcagtcagtc
     3361 ttgacctttt taccggaaaa atgtattcat taaccggacc cagtggttca ggaaaatcca
     3421 cccttgtaaa aataatatca ggttactata aaaattactt tggagacatt tatctgaatg
     3481 atatatcctt acgtaatatc agtgatgagg atttgaatga tgctatttac tacctaacac
     3541 aagatgatta tatttttatg gatacactac gatttaatct ccggctcgca aattacgacg
     3601 cgtcagaaaa tgaaatattt aaagttctta aactggcaaa tctttctgtc gtcaacaatg
     3661 aaccagtgag tctggataca caccttataa acagaggcaa taactattca ggagggcaaa
     3721 aacaacgaat ttcgttagcg cgactgtttt tgagaaaacc tgcaataatt attattgatg
     3781 aagccacatc ggctctggat tatattaatg aatcagaaat tttatcatca ataagaactc
     3841 attttcctga tgcgttaatt ataaatatta gtcaccgaat aaatcttctg gagtgttccg
     3901 attgtgttta tgtattgaat gaaggaaata ttgttgcttc tggccatttc agggatttga
     3961 tggtcagcaa tgaatacata tcgggactgg cttctgttac tgaataaaag cttgcggccg
     4021 cataatgctt aagtcgaaca gaaagtaatc gtattgtaca cggccgcata atcgaaatta
     4081 atacgactca ctatagggga attgtgagcg gataacaatt ccccatctta gtatattagt
     4141 taagtataag aaggagatat acatatggca gatctcaatt ggatatcggc cggccacgcg
     4201 atcgctgacg tcggtaccct cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc
     4261 cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag
     4321 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     4381 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4861 caatttctgg cggcacgatg gcatgagatt atcaaaaagg atcttcacct agatcctttt
     4921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5821 acggaaatgt tgaatactca tactcttcct ttttcaatca tgattgaagc atttatcagg
     5881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggtc
     5941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6781 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6841 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     6901 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     6961 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     7021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     7081 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     7141 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     7201 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     7261 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     7321 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     7381 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     7441 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     7501 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
     7561 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     7621 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     7681 gctagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     7741 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
     7801 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7861 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
     7921 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
     7981 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
     8041 agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg
     8101 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
     8161 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
     8221 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
     8281 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
     8341 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
     8401 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
     8461 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
     8521 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
     8581 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
     8641 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
     8701 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
     8761 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
     8821 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
     8881 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
     8941 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag
     9001 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgc
//
