LOCUS       V013995                 9408 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013995
VERSION     V013995
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2265..4004
                     /gene="mcjD"
                     /label="Microcin-J25 export ATP-binding/permease protein
                     McjD"
                     /note="Microcin-J25 export ATP-binding/permease protein
                     McjD from Escherichia coli. Accession#: Q9X2W0"
     promoter        4079..4097
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4098..4122
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4165..4338
                     /gene="mcjA"
                     /label="Microcin J25"
                     /note="Microcin J25 from Escherichia coli. Accession#:
                     Q9X2V7"
     CDS             4354..4398
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      4450..4497
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      4534..4989
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5107..5964)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5965..6057)
                     /label="AmpR promoter"
     rep_origin      6138..6726
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6912..7054)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(7159..7347)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(7885..7906)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7922..9001)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(9002..9079)
                     /label="lacI promoter"
ORIGIN
        1 ctctttttga tgcaatccgc tttgcttctg actataatag tcagggtaat tgagtgtaaa
       61 ggcataacta caggagggag tgtgcaaaat gatccgttac tgcttaacca gttatagaga
      121 ggatcttgtt atcctggata taattaatga tagtttcagc atagtgcctg acgcaggtag
      181 cttgctaaaa gaaagagata aattgcttaa agaattccca caactatctt acttttttga
      241 cagtgaatat catattggaa gtgtttctcg taatagtgac acttcttttc ttgaagaacg
      301 ctggtttcta ccagaacctg acaaaacatt atataagtgt tctctattta aacgatttat
      361 attattactc aaagtctttt actatagctg gaatattgaa aaaaaaggga tggcatggat
      421 tttcataagt aataaaaaag agaataggct atactccttg aatgaagagc atcttatccg
      481 gaaagaaatt agtaatcttt ccattatctt tcatcttaat atttttaaat ctgactgtct
      541 tacctattca tacgcactaa aaagaattct taattccaga aatattgatg ctcatcttgt
      601 tattggtgta aggacacaac ctttttatag ccactcttgg gtggaggttg ggggacaagt
      661 tatcaatgat gctcccaata tgcgggataa attatctgtt attgcagaga tatagttatg
      721 gaaatattta atgtcaagtt aaatgatact tcaattagaa ttattttctg taaaacgctt
      781 tctgccttcc ggacagaaaa taccatcgtt atgctcaaag gaaaagcagt ttcaaatggc
      841 aaacctgtat ccacagagga gattgccaga gtagtggaag aaaaaggtgt ttcagaagta
      901 atagaaaatt tagatggtgt tttctgtatc ctaatttatc attttaatga tctccttata
      961 gggaaaagca ttcaatcagg ccccgctcta ttttattgta aaaagaatat ggatattttt
     1021 gtttcggata aaatttctga tatcaaattt ttgaatccag atatgacatt cagtctaaat
     1081 ataacaatgg cagaacatta tctgtcagga aatcgaatag caacccagga atcactaatc
     1141 actggcattt acaaagtaaa taatggtgag tttataaaat ttaataatca gttgaaacct
     1201 gtgctacttc gtgatgagtt tagtattacc aaaaagaaca attcaactat cgacagtatc
     1261 attgataata ttgagatgat gcgggataat agaaaaatag ccctattatt ttccggagga
     1321 ttggattctg cattaatttt tcacacactt aaagaatcag gtaacaaatt ctgcgcttat
     1381 catttttttt ctgatgaatc tgatgacagt gaaaagtatt ttgctaagga atactgttca
     1441 aaatatggag ttgattttat atctgttaat aaaaacatca actttaatga aaaactttat
     1501 ttcaatttaa atcctaatag tccggacgaa atccctttga tatttgaaca gacagatgaa
     1561 gaaggtgaag gtcagccccc catagacgat gatttattat atctatgtgg tcacggtgga
     1621 gatcatattt tcggacaaaa tccttcagaa ctttttggca ttgatgcata tcgaagtcat
     1681 ggcttgatgt ttatgcataa aaaaatagta gaattttcca atctcaaggg aaagagatat
     1741 aaagatatca tattttcaaa tatttccgca ttcattaata catccaacgg atgttctcca
     1801 gcaaagcaag agcacgtatc agatatgaaa cttgcctctg ctcagttttt tgcaactgat
     1861 tatacaggaa aaattaataa actaactcca ttcctgcata aaaatattat ccagcattat
     1921 gctggcttac cagtttttag tctatttaac cagcactttg atcgttatcc cgttcgttat
     1981 gaagcgtttc aacgatttgg ttcagatatt ttctggaaaa aaaccaaacg gtcatcttca
     2041 tcatgactaa tattcagaat tctatccggt aaaaaggatg aactagtgaa tacaataaaa
     2101 cagtcaggat taattgaaat attaggcatt aaccatattg aattggaaag cattttgtat
     2161 gaaaatacga ctacacgtct gacaatggaa ctaccatata tacttaactt ataccgtctg
     2221 gcaaaattca ttcaacttca atccattgat tataaaggtt aattatggaa agaaaacaga
     2281 aaaactcatt atttaattat atttattcat taatggatgt aagaggtaaa tttttattct
     2341 tttccatgtt attcattaca tcattatcat cgataatcat atctatttca ccattgattc
     2401 ttgcaaagat tacagattta ctgtctggct cattgtcaaa ttttagttat gaatatctgg
     2461 ttttacttgc ctgtttatac atgttttgcg ttatatctaa taaagcaagt gtttttttat
     2521 ttatgatact gcaaagtagt ctacgtatta acatgcagaa aaaaatgtcg ctaaagtatt
     2581 tgagagaatt gtataacgaa aatataacta acttgagtaa aaataatgct ggatatacaa
     2641 cgcaaagtct taaccaggct tcaaatgaca tttatattct tgtgagaaat gtttcccaga
     2701 atatcctgtc acctgttata caacttattt ccactattgt tgttgtttta tctacgaagg
     2761 actggttttc tgccggtgtg ttttttctct atattctggt atttgtaatt tttaatacca
     2821 gactgactgg cagtttagcg tctctcagaa aacacagcat ggatatcact cttaactctt
     2881 atagtctgtt atctgatact gttgataaca tgatagcagc taaaaagaat aatgcattaa
     2941 gacttatttc tgaacgttat gaagatgctc tcactcagga aaacaatgct cagaaaaaat
     3001 actggttact cagttctaaa gttcttttat tgaactcttt acttgctgta atattatttg
     3061 gttctgtatt catatataat attttaggtg tgctgaatgg tgtagttagt atcggccact
     3121 tcattatgat tacatcatat atcattcttc tttcaacgcc agtggaaaat ataggggcat
     3181 tgctaagtga gatcaggcag tcaatgtcta gcctggcagg ttttattcaa cgtcatgccg
     3241 agaataaagc cacatctcct tcaatacctt ttctcaacat ggagcgaaaa ttaaacctgt
     3301 ccataagaga gctttcattt agctatagtg atgataaaaa aatacttaat tcagtcagtc
     3361 ttgacctttt taccggaaaa atgtattcat taaccggacc cagtggttca ggaaaatcca
     3421 cccttgtaaa aataatatca ggttactata aaaattactt tggagacatt tatctgaatg
     3481 atatatcctt acgtaatatc agtgatgagg atttgaatga tgctatttac tacctaacac
     3541 aagatgatta tatttttatg gatacactac gatttaatct ccggctcgca aattacgacg
     3601 cgtcagaaaa tgaaatattt aaagttctta aactggcaaa tctttctgtc gtcaacaatg
     3661 aaccagtgag tctggataca caccttataa acagaggcaa taactattca ggagggcaaa
     3721 aacaacgaat ttcgttagcg cgactgtttt tgagaaaacc tgcaataatt attattgatg
     3781 aagccacatc ggctctggat tatattaatg aatcagaaat tttatcatca ataagaactc
     3841 attttcctga tgcgttaatt ataaatatta gtcaccgaat aaatcttctg gagtgttccg
     3901 attgtgttta tgtattgaat gaaggaaata ttgttgcttc tggccatttc agggatttga
     3961 tggtcagcaa tgaatacata tcgggactgg cttctgttac tgaataaaag cttgcggccg
     4021 cataatgctt aagtcgaaca gaaagtaatc gtattgtaca cggccgcata atcgaaatta
     4081 atacgactca ctatagggga attgtgagcg gataacaatt ccccatctta gtatattagt
     4141 taagtataag aaggagatat acatatgatt aagcattttc attttaataa actgtcttct
     4201 ggtaaaaaaa ataatgttcc atctcctgca aagggggtta tacaaataaa aaaatcagca
     4261 tcgcaactca caaaaggtgg tgcaggacat gtgcctgagt attttgtggg gattggtaca
     4321 cctatatctt tctatggctg actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa
     4381 cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct
     4441 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     4501 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4561 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4621 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4681 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4741 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4801 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4861 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4921 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4981 ttacaatttc tggcggcacg atggcatgag attatcaaaa aggatcttca cctagatcct
     5041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatgattga agcatttatc
     6001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6061 gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6781 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6841 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6901 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     6961 atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     7021 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     7081 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7141 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     7201 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     7261 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     7321 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     7381 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     7441 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     7501 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     7561 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     7621 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     7681 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     7741 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     7801 catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     7861 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     7921 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7981 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     8041 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     8101 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     8161 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc
     8221 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     8281 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     8341 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     8401 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     8461 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     8521 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     8581 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     8641 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     8701 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     8761 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     8821 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     8881 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     8941 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     9001 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     9061 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     9121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     9181 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     9241 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     9301 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     9361 aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactata
//
