LOCUS       V013146                 8336 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013146
VERSION     V013146
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..100
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             119..1087
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             1118..1834
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        1909..1927
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1995..2531
                     /gene="ssbF"
                     /label="Plasmid-derived single-stranded DNA-binding
                     protein"
                     /note="Plasmid-derived single-stranded DNA-binding protein
                     from Escherichia coli (strain K12). Accession#: P18310"
     CDS             2562..3269
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      3378..3425
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3462..3917
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(4035..4892)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4893..4985)
                     /label="AmpR promoter"
     rep_origin      5066..5654
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5840..5982)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6087..6275)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(6813..6834)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6850..7929)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(7930..8007)
                     /label="lacI promoter"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattctat
      121 gaagcgtgcg cctgttattc caaaacatac gctcaatact caaccggttg gagatacttc
      181 gttatcgaca ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc
      241 tcgcggtaat gccattactt tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt
      301 gctccggggt gatagtgttg agaagacctc tcgggtatgg tcaggtaatg aacgtgacca
      361 ggagctgctt actgaggact cactggatga tctcatccct tcttttctac tgactggtca
      421 acagacaccg gcgttcggtc gaagagtatc tggtgtcata gaaattgccg atgggagtcg
      481 ccgtcgtaaa gctgctgcac ttaccgaaag tgattatcgt gttctggttg gcgagctgga
      541 tgatgagcag atggctgcat tatccagatt gggtaacgat tatcgcccaa caagtgctta
      601 tgaacgtggt cagcgttatg caagccgatt gcagaatgaa tttgctggaa atatttctgc
      661 gctggctgat gcggaaaata tttcacgtaa gattattacc cgctgtatca acacagccaa
      721 attgcctaaa tcagttgttg ctcttttttc tcaccccggt gaactatctg cccggtcagg
      781 tgatgcactt caaaaagcct ttacagataa agaggaatta cttaagcagc aggcatctaa
      841 ccttcatgag cagaaaaaag ctggggtgat atttgaagct gaagaagtta tcactctttt
      901 aacttctgtg cttaaaacgt catctgcatc aagaactagt ttaagctcac gacatcagtt
      961 tgctcctgga gcgacagtat tgtataaggg cgataaaatg gtgcttaacc tggacaggtc
     1021 tcgtgttcca actgagtgta tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc
     1081 agcacccggc ggcggaggga gtggaggagg gggaagcatg gtgagcaagg gcgaggagct
     1141 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1201 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1261 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1321 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1381 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1441 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1501 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1561 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1621 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1681 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1741 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1801 cgggatcact ctcggcatgg acgagctgta caagtgaaag cttgcggccg cataatgctt
     1861 aagtcgaaca gaaagtaatc gtattgtaca cggccgcata atcgaaatta atacgactca
     1921 ctatagggga attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag
     1981 aaggagatat acatatggca gttcgtggaa ttaacaaggt cattcttgtc ggtcgtctgg
     2041 gaaaagatcc ggaagtccgt tacatcccca acgggggcgc agtggcaaac ctgcaggtgg
     2101 ccacgtcaga aagctggcgt gacaaacaga cgggggagat gcgggagcag acagaatggc
     2161 atcgcgtggt gctgttcggc aagctcgcgg aagtggcagg tgaatatctg cgcaagggcg
     2221 cgcaggtcta catcgagggg caactccgaa cccgtagctg ggaagataac ggtatcaccc
     2281 gttacgtcac cgaaattctt gttaagacca cgggcaccat gcagatgctg ggacgtgccg
     2341 caggtgctca gactcagccg gaagaggggc aacagttcag cggtcagcct cagccggaac
     2401 cacaggcgga ggccggtacg aaaaaaggtg gcgcaaaaac gaaaggccgt ggacgtaagg
     2461 ccgcgcagcc ggagcctcag ccgcaactgc cggagggtga cgattacggg ttttcagacg
     2521 atatcccgtt cggcggcgga gggagtggag gagggggaag catggtgagc aagggcgagg
     2581 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     2641 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2701 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     2761 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     2821 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2881 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     2941 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     3001 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     3061 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     3121 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     3181 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     3241 ccaccggcgg catggacgag ctgtacaagc tcgagtctgg taaagaaacc gctgctgcga
     3301 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg
     3361 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     3421 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
     3481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3901 attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag gatcttcacc
     3961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc atgattgaag
     4921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4981 acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5761 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5821 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5881 cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5941 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     6001 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6061 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6121 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     6181 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     6241 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     6301 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     6361 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     6421 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     6481 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     6541 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     6601 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     6661 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     6721 agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     6781 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     6841 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6901 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     6961 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     7021 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     7081 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac
     7141 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     7201 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     7261 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     7321 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     7381 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     7441 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     7501 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     7561 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     7621 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     7681 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     7741 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     7801 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     7861 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     7921 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     7981 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     8041 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     8101 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     8161 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     8221 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     8281 cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actata
//
