LOCUS       62056_10295        5344 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(404..451)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(516..542)
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        complement(1620..1648)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3195..3383
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3488..3630
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3816..4404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4526..5338
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatccagcgt aatctggtac gtcgtatggg
      541 tatttacccg gagacagaga cagagatttc tgggtgtagt ggttgtgcag agcttcgtgc
      601 ataacagagc aagagaaaac gttaccctgc tgccaacgag atttgtcaac ggtcagttta
      661 gagtacagga agaaagaacc gtcagagtcc agaaccggcg gggtggtttt gtagttgttt
      721 tccggctgac cgttagattc ccattcaaca gcgatgtcag acgggtagaa acctttaacc
      781 aggcaggtca gagaaacctg gtttttggtc agttcgtcac gagacggcgg cagggtgtaa
      841 acctgcggtt cacgcggctg acctttagct ttagagatgg ttttttcgat cggagccggc
      901 agagctttgt tagaaacttt gcatttgtat tctttaccgt tcagccagtc ctggtgcaga
      961 acggtcagaa cagaaacaac acggtaggta gagttgtact gttcttcacg cggtttggtt
     1021 ttagcgttgt gaacttcaac accgtcaacg taccagttga atttaacttc cgggtcttcg
     1081 tgagaaacgt caacaacaac gcaggtaact tccggggtac gagagatcat cagggtgtct
     1141 ttcggtttcg gcgggaacag gaaaacagac ggaccaccca gcagttccgg agccgggcac
     1201 ggcgggcagg tgtgggtttt gtcgcaagat ttcggttcag aaacggtaac ctgggtaccc
     1261 tgaccccagt actggaaagc accagaagag gtaaccaggg tgcaggtcgg gtcttcgaaa
     1321 gagtcagcag cgcagtagta catagcggtg tcttccggtt tcagagagtt catctgcagg
     1381 taaacggtag atttagcgtt gttctgagag atggtgaaac gacctttaac agagtcagcc
     1441 aggtaggtag aaccagaggt ggtcagcagt ttagcaacac gttcacgttc tttacccgga
     1501 gcctgacgga accaagccat gcaacgacga gaagacattt taccagaagc agcgcaagac
     1561 agacgcagag aaccacccgg ctgaaccaga ccaccaccag attcctgcag ctgaacctgc
     1621 attatacgag ccgatgatta attgtcaaag atctcgatcc tctacgccgg acgcatcgtg
     1681 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     1741 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     1801 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     1861 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     1921 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     1981 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2041 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     2101 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     2161 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     2221 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     2281 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     2341 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     2401 ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     2461 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     2521 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     2581 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     2641 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     2701 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     2761 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     2821 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     2881 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
     2941 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     3001 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     3061 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     3121 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
     3181 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     3241 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     3301 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
     3361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3481 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     3541 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
     3601 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     3661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
     4501 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag
     4561 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     4621 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     4681 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     4741 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     4801 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
     4861 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     4921 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     4981 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     5041 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
     5101 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     5161 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     5221 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     5281 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     5341 agaa
//
