LOCUS       V014280                 5816 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014280
VERSION     V014280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(165..644)
                     /gene="TNNC1"
                     /label="Troponin C, slow skeletal and cardiac muscles"
                     /note="Troponin C, slow skeletal and cardiac muscles from
                     Oryctolagus cuniculus. Accession#: P02591"
     CDS             complement(654..686)
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(690..707)
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             complement(717..734)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     RBS             complement(753..775)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(790..814)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(815..833)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1142..1219
                     /label="lacI promoter"
     CDS             1220..2299
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    2315..2336
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3111..3299
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3404..3546
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3732..4320)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             4442..5254
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(5350..5805)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtctccac acccttcatg
      181 aactccagga actcatcata gtcgatgcgg ccgtcgttgt tcttgtctcc gtccttcatg
      241 agctcctcga tgtcgtcctc cgtgatggtc tcgcctgtag cctgcagcat tatcttcagc
      301 tcatccaggt cgatgtagcc atcagcattt ttgtcaaaca tgcggaagag gtcagacagc
      361 tcctcctcag atttcccttt gctgtcgtcc ttcatgcacc gaaccatcat gaccaggaac
      421 tcatcaaagt ccaccgtgcc gctgccgtcc tcgtccacct catcgatcat ctcctgcagc
      481 tcctcagggg tggggttctg gcccagcatc ctcatcacct tgcccagctc cttggtgctg
      541 atgcagccat cctcagcgcc cagcacgaag atgtcgaagg ctgccttgaa ctcatttttc
      601 tgctcttctg tcagctgctc taccgcagcc ttgtagatgt catcggatcc gcgacccatt
      661 tgctgtccac cagtcatgct agccatatgg ctgccgcgcg gcaccaggcc gctgctgtga
      721 tgatgatgat gatggctgct gcccatggta tatctccttc ttaaagttaa acaaaattat
      781 ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc
      841 gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
      901 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
      961 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     1021 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     1081 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc
     1141 ggacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     1201 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     1261 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     1321 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     1381 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     1441 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     1501 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     1561 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     1621 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     1681 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     1741 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     1801 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     1861 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     1921 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     1981 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     2041 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     2101 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     2161 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     2221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2281 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg
     2341 caccgggatc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     2401 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     2461 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     2521 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     2581 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     2641 ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     2701 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     2761 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     2821 gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat
     2881 gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct
     2941 gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt
     3001 ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
     3061 tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac
     3121 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
     3181 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
     3241 acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     3301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     3361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     3421 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     3481 gagagtgcac catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3541 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4141 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4381 gggattttgg tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     4441 tatgagccat attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga
     4501 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     4561 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     4621 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     4681 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     4741 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     4801 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     4861 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     4921 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     4981 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     5041 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     5101 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     5161 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     5221 attgcagttt catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca
     5281 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5341 tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5401 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5461 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5521 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5581 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5641 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5701 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5761 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//
