LOCUS       V013076                 4968 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013076
VERSION     V013076
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        3380..3408
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3456..3488
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             3498..4742
                     /gene="ndh"
                     /label="Type II NADH:quinone oxidoreductase"
                     /note="Type II NADH:quinone oxidoreductase from Escherichia
                     coli (strain K12). Accession#: P00393"
     CDS             4743..4775
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     CDS             4812..4829
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      4896..4943
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctccgggat ctcgacgctc tcccttatgc
     3361 gactcctgca ttaggaaatt tgacaattaa tcatcggctc gtataatgtg tggaattctc
     3421 tgagcggata tttcacacag gaaacagtat tccatatggc tagcatgact ggtggacagc
     3481 aaatgggtcg ggatcctatg gcaacacagc tggggcataa gctgggacgc aagaaaaaag
     3541 ccaaaattac gctggtcgat cgtaaccaca gccacctgtg gaaaccgctg ctgcacgaag
     3601 tggcgactgg ctcgcttgat gaaggcgtcg atgcgttgag ctatctggcc catgcgcgca
     3661 atcatggttt ccagttccag ctgggttccg tcattgatat tgatcgtgaa gcgaaaacaa
     3721 tcactattgc agaactgcgc gacgagaaag gtgaactgct ggttccggaa cgtaaaatcg
     3781 cctatgacac cctggtaatg gcgctgggta gcacctctaa cgatttcaat acgccaggtg
     3841 tcaaagagaa ctgcattttc ctcgataacc cgcaccaggc gcgtcgcttc caccaggaga
     3901 tgctgaattt gttcctgaaa tactccgcca acctgggcgc gaatggcaaa gtgaacattg
     3961 cgattgtcgg cggcggcgcg acgggtgtag aactctccgc tgaattgcac aacgcggtca
     4021 agcaactgca cagctacggt tacaaaggcc tgaccaacga agccctgaac gtaacgctgg
     4081 tagaagcggg agaacgtatt ttgcctgcgt taccgccacg tatctctgct gcggcccaca
     4141 acgagctaac gaaacttggc gttcgcgtgc tgacgcaaac catggtcacc agtgctgatg
     4201 aaggcggcct gcacactaaa gatggcgaat atattgaggc tgatctgatg gtatgggcag
     4261 ccgggatcaa agcgccagac ttcctgaaag atatcggtgg tcttgaaact aaccgtatca
     4321 accagctggt ggtggaaccg acgctgcaaa ccacccgcga tccagacatt tacgctattg
     4381 gcgactgcgc gtcatgcccg cgtccggaag ggggctttgt tccgccgcgt gctcaggctg
     4441 cacaccagat ggcgacttgc gcaatgaaca acattctggc gcagatgaac ggtaagccgc
     4501 tgaaaaatta tcagtataaa gatcatggtt cgctggtatc gctgtcgaac ttctccaccg
     4561 tcggtagcct gatgggtaac ctgacgcgcg gctcaatgat gattgaagga cgaattgcgc
     4621 gctttgtata tatctcgcta taccgaatgc atcagattgc gctgcatggt tactttaaaa
     4681 ccggattaat gatgctggtg gggagtatta accgcgttat ccgtccgcgt ttgaagttgc
     4741 atgcagcgaa cgacgaaaat tacgcccttg cagcgaagag ctccgtcgac aagcttgcgg
     4801 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
     4861 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     4921 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
//
