LOCUS       V013556                16194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013556
VERSION     V013556
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..16194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3614..3635)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3651..4730)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4731..4808)
                     /label="lacI promoter"
     promoter        5117..5135
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5136..5160
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5175..5197
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9109..13407
                     /gene="rpoC"
                     /label="DNA-directed RNA polymerase subunit beta'"
                     /note="DNA-directed RNA polymerase subunit beta' from
                     Porphyromonas gingivalis (strain ATCC BAA-308 / W83).
                     Accession#: Q7MX26"
     CDS             13408..13425
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        13435..13453
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    13454..13478
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             13493..13515
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             13523..14512
                     /gene="rpoA"
                     /label="DNA-directed RNA polymerase subunit alpha"
                     /note="DNA-directed RNA polymerase subunit alpha from
                     Porphyromonas gingivalis (strain ATCC BAA-308 / W83).
                     Accession#: Q7MTP0"
     CDS             14610..15599
                     /gene="rpoA"
                     /label="DNA-directed RNA polymerase subunit alpha"
                     /note="DNA-directed RNA polymerase subunit alpha from
                     Porphyromonas gingivalis (strain ATCC BAA-308 / W83).
                     Accession#: Q7MTP0"
     CDS             16038..16055
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      16122..16169
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgacc ccgaccacca
     5221 acaacaaacg tatcaacttc gcttctatca aaaacccgct gttctacccg gacttcctgg
     5281 aagttcagct gaaatctttc cacgacttcc tgcagctgga caccccgccg gaacgtcgta
     5341 aaaaagaagg tctgtacaaa gttttcgctg aaaacttccc gatcaccgac acccgtaaca
     5401 acttcgttct ggaattcctg gactactaca tcgacccgcc gaaatactct atcgaagaat
     5461 gcctgtctcg tggtctgacc tactctgttc cgctgaaagc taaactgaaa ctgtactgca
     5521 ccgacccgga ccacgaagac ttcgctaccg ttatccagga cgttttcctg ggtccgatcc
     5581 cgtacatgac ctcttctggt accttcgtta tcaacggtgc tgaacgtgtt atcgtttctc
     5641 agctgcaccg ttctccgggt gttttcttcg gtcagtctct gcacaccaac ggtaccaaac
     5701 tgtactctgc tcgtatcatc ccgttcaaag gttcttggat cgaattcgct accgacatca
     5761 acaacgttat gtacgcttac atcgaccgta aaaaaaaact gccggttacc accctgctgc
     5821 gtgctatcgg tttcgaagct gacaaagaca tcctggacat cttcaacctg gctgaagaag
     5881 ttaaagttac caaagctaac ctgaaaaaat gcatcggtcg taaactggct gctcgtgtta
     5941 tcaacaccta catcgacgac ctgtctgacg aagacaccgg tgaagttgtt tctatggaac
     6001 gtatcaccgt tgttgttgac cgtgaagttg aactgaccga agacaacatc gaagctatcc
     6061 tgaactctaa cacccagacc atcctgctgc accgtaacga ctctaacacc tctgactact
     6121 ctatcatctt caacaccctg cagaaagacc cgtgcaactc tgaaaaagaa gctctgtact
     6181 acgtttaccg tcagctgcgt aacgctgaac cggctgacga cgcttctgct cgtgaagtta
     6241 tcaccaacct gttcttctct gacaaacgtt acgacctggg tgacgttggt cgttaccgta
     6301 tcaacaaaaa actgaacctg aacatcgacc cggacatcaa agttctgacc aacgaagaca
     6361 tcatcgaaat catcaaatac ctgatcgaac tggttaactc taaagcttct gttgacgaca
     6421 tcgaccacct gtctaaccgt cgtgttcgta ccgttggtga acagctgtac aaccagttcg
     6481 gtatcggtct ggctcgtatg gctcgtaccg ttcgtgaccg tatgaacgtt cgtgacaacg
     6541 aagttttcac cccgatcgac ctggttaacg ctaaaaccat ctcttctgtt gttaactctt
     6601 tcttcggtac caacgctctg tctcagttca tggaccagac caacccgctg gctgaaatca
     6661 cccacaaacg tcgtctgtct gctctgggtc cgggtggtct gtctcgtgaa cgtgctggtt
     6721 tcgaagttcg tgacgttcac tacacccact acggtcgtct gtgcccgatc gaaaccccgg
     6781 aaggtccgaa catcggtctg atctcttctc tgtgcgttta cgctaaaatc tctgacctgg
     6841 gtttcatcac caccccgtac cgtgaagtta aaaacggtaa agttgacttc tctgacaacg
     6901 gtctgaaata ctacaccgct gaagaagaag aagaaaaaac cgttgctcag ggtaacgctc
     6961 cgctggacga aaacggtcgt ttcgttcgtg aacgtgttaa agctcgttac gaatctgact
     7021 tcccgctggt taccccggac gaagttgacc tgatggacgt ttctccgacc cagatcgctt
     7081 ctatcgctgc tgctctgatc ccgttcctgg aacacgacga cgctaaccgt gctctgatgg
     7141 gttctaacat gatgcgtcag gctgttccgc tgctgcgtcc ggaatctccg atcgttggta
     7201 ccggtatcga aggtaaactg gttaaagact ctcgtaccca gatcgttgct gaacgtggtg
     7261 gtgaagttgt tttcgttgac gcttcttgca tcaaaatccg ttacgaccgt accgctgacg
     7321 aagaattcgt ttctttcgac gacgctatcg ttacctacta cctgccgaaa taccgtaaaa
     7381 ccaaccagtc taccaccatc gacctgcacc cgatctgctc taaaggtgac cgtgttgaag
     7441 ctggtcagat cctgaccgaa ggttactcta cccagggtgg tgaactggct ctgggtcgta
     7501 acgttcaggt tgcttacatg ccgtggaaag gttacaacta cgaagacgct atcgttctga
     7561 acgaacgtat ggttcgtgaa gacttcttca cctctgttca cgttgacgaa tacatcctgg
     7621 aagttcgtga aaccaaacgt ggtctggaag aactgacctc tgacatcccg aacgtttctg
     7681 aagacgctac ccgtgacctg gacgaaaacg gtatcgttcg tatcggtgct cacatcgaac
     7741 cgggtgacat cctgatcggt aaaatcaccc cgaaaggtga atctgacccg accccggaag
     7801 aaaaactgct gcgtgctatc ttcggtgaca aagctggtga cgttaaagac gcttctctga
     7861 aagctacccc gtctctgcgt ggtgttgtta tcgacaccaa actgttctct aaagctgcta
     7921 aaaaaaaatc tcgtacctct accaaagaag ctgtttctaa actggacgaa acctacgcta
     7981 aacgtcagca gcagctgcac gaacgtctga tcgacaaact gaccgaactg accaaaggta
     8041 aaacctgctg cggtgttaaa gactacctga acgttgaact gatcaaagct ggttctaaat
     8101 tcgctaaaaa agacctggaa gctctggact tcaacgttat ccagctgtct gactggacca
     8161 acgacgctca caccaacgaa ctgatcaaag ctgttgctgt taactacctg aaacactcta
     8221 aagaaatcga agctgaactg cgtcgtcgta aactggacga aaccatcggt gacgaactgc
     8281 cggctggtat cgttcagatg gctaaagttt acatcgctaa aaaacgtaaa atccaggttg
     8341 gtgacaaaat ggctggtcgt cacggtaaca aaggtatcgt ttctaaaatc gttcgtcagg
     8401 aagacatgcc gttcctggct gacggtaccc cggttgacat ctgcctgaac ccgctgggtg
     8461 ttccgtctcg tatgaacctg ggtcagatct tcgaagctgt tctggcttgg gctggtcgta
     8521 aaatgaacgt taaattcgct accccgatct tcgacggtgc ttctctgaac gacatgaacg
     8581 aatggaccga caaagctggt ctgccgcgtg acggtaaaac ctacctgtac gacggtggta
     8641 ccggtgaacg tttcgaccag ccggctaccg ttggtgttac ctacttcctg aaactgggtc
     8701 acatggttga cgacaaaatg cacgctcgtt ctatcggtcc gtactctctg atcacccagc
     8761 agccgctggg tggtaaagct cagttcggtg gtcagcgttt cggtgaaatg gaagtttggg
     8821 ctctggaagc tttcggtgct tctcacatcc tgcaggaaat cctgaccgtt aaatctgacg
     8881 acgttgttgg tcgttctaaa gcttacgaag ctatcgttaa aggtgacccg atgccgaccc
     8941 cgggtatccc ggaatctctg aacgttctgc tgcacgaact gaaaggtctg ggtctgtctt
     9001 tctctctgga ctgaggatcc taatacgact cactataggg gaattgtgag cggataacaa
     9061 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggctttccgt
     9121 aaagaaaaca aaatcaaaaa caacttctct aaaatccgta tcaccctggc ttctccggaa
     9181 gaaatcctgg aaaactcttt cggtgaagtt ctgaaaccgg aaaccatcaa ctaccgtacc
     9241 tacaaaccgg aacgtgacgg tctgttctgc gaacgtatct tcggtccggt taaagacttc
     9301 gaatgccact gcggtaaata caaacgtatc cgttaccgtg gtatcgtttg cgaccgttgc
     9361 ggtgttgaag ttaccgaaaa aaaagttcgt cgtgaacgta tgggtcacat ccacctggtt
     9421 gttccggttg ctcacatctg gtacttccgt tctctgccga acaaaatcgg ttacctgctg
     9481 ggtctgccga ccaaaaaact ggacgctatc atctactacg aacgttacgt tgttatccag
     9541 ccgggtgttg ctgaaggtct gtctcagctg gacctgctgt ctgaagaaga atacctggac
     9601 aaactggacg aaatcgaacg tacccacaaa ggtaaccaga acctggaaga caccaacccg
     9661 gacaaattca tcgctaaaat cggtgctgaa gctatctacg acctgctgtg ccgtgttgac
     9721 ctggactcta tctcttacga actgcgtgac cgtgctaaca ccgacggttc tcagcagcgt
     9781 aaaaccgaag ctctgaaacg tctgcaggtt gttgaatctt tccgtgcttc taaaggtgtt
     9841 aaccgtccgg aatggatggt tatgaaagtt atcccggtta tcccgccgga cctgcgtccg
     9901 ctggttccgc tggacggtgg tcgtttcgct acctctgacc tgaacgacct gtaccgtcgt
     9961 gttatcatcc gtaacaaccg tctgaaacgt ctgatcgaaa tcaaagctcc ggaagttatc
    10021 ctgcgtaacg aaaaacgtat gctgcaggaa gctgttgact ctctgttcga caactctcgt
    10081 aaatcttctg ctgttaaatc tgacaacaac cgtccgctga aatctctgtc tgactctctg
    10141 aaaggtaaac agggtcgttt ccgtcagaac ctgctgggta aacgtgttga ctactctgct
    10201 cgttctgtta tcgttgttgg tccggaactg aaaatgcacg aatgcggtct gccgaaagac
    10261 atggctgctg aactgtacaa accgttcatc atccgtaaac tgatcgaacg tggtatcgtt
    10321 aaaaccgtta aatctgctaa aaaaatcgtt gaccgtaaag aaccggttat ctgggacatc
    10381 ctggaatacg ttatgaaagg tcacccggtt ctgctgaacc gtgctccgac cctgcaccgt
    10441 ctgggtatcc aggctttcca gccgaaactg atcgaaggta aagctatcca gctgcacccg
    10501 ctgtcttgca ccgctttcaa cgctgacttc gacggtgacc agatggctgt tcacctgccg
    10561 ctgtctaacg aagctatcct ggaagctcag ctgctgatgc tggcttctca caacatcctg
    10621 aacccggcta acggtgctcc gatcaccgtt ccgtctcagg acatggttct gggtctgtac
    10681 tacatcacca aactgcgtcc gaacaccaaa ggtcacggtc tgatcttcta cggtccggaa
    10741 gaagctacca tcgcttacaa cgaaggtaaa gttgacatcc acgctccgat caaagtttac
    10801 gttgaagact acgaaaacgg tgaactggtt cgtcgtatgg ttgaaacctc tgttggtcgt
    10861 ctgatggtta acgaatacgt tccgaaaaaa gttggttacg ttaacgaagt tctgggtaaa
    10921 aaagctctgc gtgacatcat cggttctgtt atcaaaatct gcggtgttgc taccaccgct
    10981 aaattcctgg acgacatcaa aaacctgggt tactacatgg ctttcaaagg tggtctgtct
    11041 ttcaacctgg ctgacgttct gatcccggac gaaaaagacc agctgatcca ggaaggttac
    11101 accgctgttg aacagatcat gcaggactac tctatgggtt tcatcacctt caacgaacgt
    11161 tacaaccaga tcatcgacac ctggacccac atcaacggtc gtctgtctaa cgttctgatc
    11221 aaacagctgt cttctgacaa cgacggtttc aactctgttt tcatgatgat ggactctggt
    11281 gctcgtggtt ctaaagaaca gatccgtcag ctgtctggta tgcgtggtct gatggctaaa
    11341 ccgcagaaat ctggtgctga aggtggtcag atcatcgaaa acccgatcct gtctaacttc
    11401 aaagaaggtc tgtctgttct ggaatacttc atctctaccc acggtgctcg taaaggtctg
    11461 gctgacaccg ctctgaaaac cgctgacgct ggttacctga cccgtcgtct ggttgacgtt
    11521 tctcacgacg ttatcatcac cgaagaagac tgcggtaccc tgcgtggtct gctgaccacc
    11581 gaactgaaac agaacgaaga cgttgttgct tctctgtacg aacgtatcct gggtcgtgtt
    11641 tctgttcacg acatcatcca cccgaccacc ggtgacatca tcgttcgtgc tggtgaagaa
    11701 atccgtgaac aggctgctca gatcatcgaa gactctccga tcgaagctgt tgaaatccgt
    11761 tctgttctga cctgcgaatc taaaaaaggt gtttgcgcta aatgctacgg tcgtaacctg
    11821 gctaccaacc gtatggttca gcgtggtgaa gttgttggtg ttatcgctgc tcagtctatc
    11881 ggtgaaccgg gtacccagct gaccctgcgt accttccacg ttggtggtat cgcttctaac
    11941 gttgctaccg aaaactctct gctgtctaaa tacgacggta tcctggaatt cgaagaactg
    12001 cgtgctgttg acgttaccga cgaatctcac caggttgttg tttctcgtat gaccgaactg
    12061 cgtatcgctg acccgaacac cggtatcatc ctggctaacc acaacatccc gtacggtgct
    12121 aaactgttct tccgtcaggg tgacgctgtt aaaaaaggtg acaaaatcat cgaatgggac
    12181 ccgttcaacg ctgttatcgt ttctgaagtt gctggtaccc tgtctttcga aggtgttgtt
    12241 gaaaacgtta ccttcaaaat ggaatctgac gaaaccaccg gtctgaaaga aaaaatcatc
    12301 atcgaatcta aagacaaaac catggctccg tacgctcgta tcatcgacga aaacggtgaa
    12361 atgctgaaaa actactctct gccgatgggt gctcacgttg ttaaagacga cggtgacacc
    12421 gttaaagttg gtgaaatcct ggttaaaatc ccgcgttctg ttggtaaagc tggtgacatc
    12481 accggtggtc tgccgcgtgt taccgaactg ttcgaagctc gtaacccgtc taacccggct
    12541 atcgtttctg aaatcgacgg tgaaatcggt ttcggtaaac tgaaacgtgg taaccgtgaa
    12601 atcaccgtta cctctaaact gggtgaagaa aaaaaatacc tgatcccgct gtctaaacag
    12661 ctgctggttc aggaaaacga cttcgttcgt gctggtaccc cgctgtctga cggtgctatc
    12721 accccggctg acatcctggc tatcaaaggt ccgaccgctg ttcaggaata catcgttaac
    12781 gaagttcagg acgtttaccg tctgcagggt gttaaaatca acgacaaaca cttcgaagtt
    12841 atcgttcgtc agatgatgcg taaagttgaa atcgttgacc cgggtgacac cctgttcctg
    12901 gaacagcagg ttgttgacaa attcgaagtt atggaagaaa acgaccgtat ctggggtaaa
    12961 aaagttgtta tcgacgctgg tgactctcag gttctgaaag ctggtcagat cgttaccgct
    13021 cgtaaactgc gtgacgaaaa ctctatgctg aaacgtaaag acctgaaaat cgttaaagtt
    13081 cgtgacgcta aatctgctac cgcttctcag atcctgcagg gtatcacccg tgctgctctg
    13141 cagaccaaat ctttcatgtc tgctgcttct ttccaggaaa ccaccaaagt tctgaacgaa
    13201 gctgctatct gcggtaaaac cgactacctg gaaggtctga aagaaaacgt tatctgcggt
    13261 cacctgatcc cggctggtac cggtctgcgt gactacgaaa aactggttgt tatgcaccgt
    13321 gacgactacg aaaaagctac cgctgaacgt aaatctttcc tgtctgttcc gaccgctgaa
    13381 ccggctatgg aagaagctcc gtctgaacac caccaccacc accactgaga gctctaatac
    13441 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
    13501 actttaagaa ggagatatac atatggctat cctggctttc cagaaaccgg aaaacgttct
    13561 gatgatggaa acctctgact ctatcgctaa attcgaattc aaaccgctgg aaccgggtta
    13621 cggtatcacc atcggtaacg ctctgcgtcg tatcctgctg tcttctctgg aaggtttcgc
    13681 tatcaccgct atcaaaatcg aaggtgttga acacgaattc gctaccatcc cgggtgttct
    13741 ggaagacgtt accaacatca tcctgaacct gaaacaggtt cgtttcaaac agatcgttcc
    13801 gaacgctgac gttgaaaaag ctaccatcgt tatctctaac tctgaagttt tccgtgctgg
    13861 tgacctgaac gctcagctgt ctaacttcga agttctgaac tctaacctgg ttatctgcca
    13921 cctggacaaa tctgctaccc tgaccatgga attctctatc aacaaaggtc gtggttacgt
    13981 ttctgctgaa gaaaaccgtg ctgaacacaa cgaactgtct accatcgcta tcgactctat
    14041 ctacaccccg atccgtaacg ttaaatacgc tgttgaaaac ttccgtgttg aacagaaaac
    14101 cgactacgaa aaactgctga tggaagttac caccgacggt tctatccgtc cggttgacgc
    14161 tctgcgtgaa gctgctcaga tcctgatctc tcacttctct ctgttcgctg aaaacaaaat
    14221 cgctatcgaa tacgttgaca tcgttgacac cgacgaattc gacgaagact ctctgcacat
    14281 gcgtcagctg ctgaaatcta aactgtctgg tctggacctg tctgttcgtg ctctgaactg
    14341 cctgaacgct gctggtgttg acaccctggg tgacctggtt tctctgtctc gttctgacct
    14401 gatgaaaatc cgtaacttcg gtaaaaaatc tctgaccgaa ctggacgaac tgctggctac
    14461 cctgaacctg tctttcggta tggacatctc taaatacaaa ctggacaaag actgagtcga
    14521 ctaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
    14581 ttgtttaact ttaagaagga gatatacata tggctatcct ggctttccag aaaccggaaa
    14641 acgttctgat gatggaaacc tctgactcta tcgctaaatt cgaattcaaa ccgctggaac
    14701 cgggttacgg tatcaccatc ggtaacgctc tgcgtcgtat cctgctgtct tctctggaag
    14761 gtttcgctat caccgctatc aaaatcgaag gtgttgaaca cgaattcgct accatcccgg
    14821 gtgttctgga agacgttacc aacatcatcc tgaacctgaa acaggttcgt ttcaaacaga
    14881 tcgttccgaa cgctgacgtt gaaaaagcta ccatcgttat ctctaactct gaagttttcc
    14941 gtgctggtga cctgaacgct cagctgtcta acttcgaagt tctgaactct aacctggtta
    15001 tctgccacct ggacaaatct gctaccctga ccatggaatt ctctatcaac aaaggtcgtg
    15061 gttacgtttc tgctgaagaa aaccgtgctg aacacaacga actgtctacc atcgctatcg
    15121 actctatcta caccccgatc cgtaacgtta aatacgctgt tgaaaacttc cgtgttgaac
    15181 agaaaaccga ctacgaaaaa ctgctgatgg aagttaccac cgacggttct atccgtccgg
    15241 ttgacgctct gcgtgaagct gctcagatcc tgatctctca cttctctctg ttcgctgaaa
    15301 acaaaatcgc tatcgaatac gttgacatcg ttgacaccga cgaattcgac gaagactctc
    15361 tgcacatgcg tcagctgctg aaatctaaac tgtctggtct ggacctgtct gttcgtgctc
    15421 tgaactgcct gaacgctgct ggtgttgaca ccctgggtga cctggtttct ctgtctcgtt
    15481 ctgacctgat gaaaatccgt aacttcggta aaaaatctct gaccgaactg gacgaactgc
    15541 tggctaccct gaacctgtct ttcggtatgg acatctctaa atacaaactg gacaaagact
    15601 gagcggccgc taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
    15661 gaaataattt tgtttaactt taagaaggag atatacatat ggaactgaaa aaaatgaacg
    15721 ttccgatgga caccatcacc cgtgacatgg ttcgtctgtc tgaagacacc gaaaacgttt
    15781 acgaaaccgt tatgatcatc gctaaacgtg ctaaccagat cggtcagcag atgaaacagg
    15841 acctggaaaa aaaactgcag gacttctctt cttctaacga caacctggaa gaagttttcg
    15901 aaaaccgtga acagatcgaa atctctcgtt actacgaaca cctgccgaaa ccgggtctga
    15961 tcgctaccgc tgaatacgaa caggacaaac tgtaccaccg tatgccgggt gctacctcta
    16021 ccaacgactg actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
    16081 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
    16141 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat
//
