LOCUS       V015146                 6647 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015146
VERSION     V015146
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        3348..3366
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3398..3420
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     3428..3493
                     /label="pelB signal sequence"
                     /note="leader peptide for secretion"
     CDS             3527..6463
                     /gene="PTPRN"
                     /label="Receptor-type tyrosine-protein phosphatase-like N"
                     /note="Receptor-type tyrosine-protein phosphatase-like N
                     from Homo sapiens. Accession#: Q16849"
     CDS             6491..6508
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6575..6622
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
     3361 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
     3421 tatacatatg aaatacctgc tgccgaccgc tgctgctggt ctgctgctcc tcgctgccca
     3481 gccggcgatg gccatggata tcggaattaa ttcggatccg aattcgatgc gtcgtccacg
     3541 tcgtccaggt ggtcttggtg gttcaggtgg tcttcgtctt cttctttgtc ttcttcttct
     3601 ttcatcacgt ccaggtggtt gttcagctgt ttcagctcac ggttgtcttt tcgatcgtcg
     3661 tctttgttca caccttgaag tttgtatcca agatggtctt ttcggtcaat gtcaagttgg
     3721 tgttggtcaa gctcgtccac ttcttcaagt tacttcacca gttcttcaac gtcttcaagg
     3781 tgttcttcgt caacttatgt cacaaggtct ttcatggcac gatgatctta ctcaatacgt
     3841 tatctcacaa gaaatggaac gtatcccacg tcttcgtcca ccagaaccac gtccacgtga
     3901 tcgttcaggt cttgctccaa aacgtccagg tccagctggt gaacttcttc ttcaagatat
     3961 accaactggt tcagctccag ctgctcaaca ccgtcttcca caaccaccag ttggtaaagg
     4021 tggtgctggt gcttcgtcgt cactcagccc acttcaagct gaacttcttc caccacttct
     4081 tgaacacctt cttcttccac cacaaccacc acacccatca ctttcatacg aaccagctct
     4141 tcttcaacca taccttttcc accaattcgg ttcaagggat ggttcgcgtg taagcgaagg
     4201 ttcaccaggt atggtttcag ttggtccact tccaaaagcg gaagctccag ctctgttcag
     4261 ccgtactgct tcaaaaggta tcttcggtga tcacccaggt cactcatacg gtgatcttcc
     4321 aggtccatca ccagctcaac ttttccaaga ttcaggtctt ctttaccttg ctcaagaact
     4381 tccagctcca tcacgtgctc gtgttccacg tcttccagaa caaggttcat catcacgtgc
     4441 tgaagattca ccagaaggtt acgaaaaaga aggtcttggt gatcgtggtg aaaaaccagc
     4501 ttcaccagct gttcaaccag atgctgctct tcaacgtctt gctgctgttc ttgctggtta
     4561 cggtgttgaa cttcgtcaac ttactccaga acaactttca actcttctta ctcttcttca
     4621 acttcttcca aaaggtgctg gtcgtaaccc aggtggtgtt gttaacgttg gtgctgatat
     4681 aaaaaaaact atggaaggtc cagttgaagg tcgtgatact gctgaacttc cagctcgtac
     4741 ttcaccaatg ccaggtcacc caactgcttc accaacttca tcagaagttc aacaagttcc
     4801 atcaccagtt tcatcagaac caccaaaagc tgctcgtcca ccagttactc cagttcttct
     4861 tgaaaaaaaa tcaccacttg gtcaatcaca accaactgtt gctggtcaac catcagctcg
     4921 tccagctgct gaagaatacg gttacatcgt tactgatcaa aaaccacttt cacttgctgc
     4981 tggtgttaaa cttcttgaaa tccttgctga acacgttcac atgtcatcag gttcattcat
     5041 caacatctca gttgttggtc cagctcttac tttccgtatc cgtcacaacg aacaaaacct
     5101 ttcacttgct gatgttactc aacaagctgg tcttgttaaa tcagaacttg aagctcaaac
     5161 tggtcttcaa atccttcaaa ctggtgttgg tcaacgtgaa gaagctgctg ctgttcttcc
     5221 acaaactgct cactcaactt caccaatgcg ttcagttctt cttactcttg ttgctcttgc
     5281 tggtgttgct ggtcttcttg ttgctcttgc tgttgctctt tgtgttcgtc aacacgctcg
     5341 tcaacaagat aaagaacgtc ttgctgctct tggtccagaa ggtgctcacg gtgatactac
     5401 tttcgaatac caagatcttt gtcgtcaaca catggctact aaatcacttt tcaaccgtgc
     5461 tgaaggtcca ccagaaccat cacgtgtttc atcagtttca tcacaattct cagatgctgc
     5521 tcaagctagt ccatcatcac actcatcaac tccatcatgg tgtgaagaac cagctcaagc
     5581 taacatggat atatcaactg gtcacatgat ccttgcttac atggaagatc accttcgtaa
     5641 ccgtgatcgt cttgcgaaag aatggcaggc tctctgcgct taccaagctg aaccaaacac
     5701 ttgtgctact gctcaaggtg aaggtaacat caaaaaaaac cgtcacccag atttccttcc
     5761 atacgatcac gctcgtatca aacttaaagt tgaatcatca ccatcacgtt cagattacat
     5821 caacgcttca ccaatcatcg aacacgatcc acgtatgcca gcttacatcg ctactcaagg
     5881 tccactttca cacactatcg ctgatttctg gcaaatggtt tgggaatcag gttgtactgt
     5941 tatcgttatg cttactccac ttgttgaaga tggtgttaaa caatgtgatc gttactggcc
     6001 agatgaaggc gcgtcactct accacgtgta cgaagttaac cttgtttcag aacacatctg
     6061 gtgcgaagac ttccttgttc gttcgttcta ccttaaaaac gttcaaactc aagaaactcg
     6121 tactcttact caattccact tcctttcatg gccagctgaa ggtactccag cttcaactcg
     6181 tccacttctt gatttccgtc gtaaagttaa caaatgttac cgtggtcgtt catgtccaat
     6241 catcgttcac tgttcagatg gtgctggtcg tactggtact tacatcctta tcgatatggt
     6301 tcttaaccgt atggctaaag gtgttaaaga aatcgatata gctgctactc ttgaacacgt
     6361 tcgtgatcaa cgtccaggtc ttgttcgttc aaaagatcaa ttcgaatttg ctcttactgc
     6421 tgttgctgaa gaagttaacg ctatccttaa agctcttcca caagtcgaca agcttgcggc
     6481 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
     6541 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     6601 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
//
