LOCUS       V013688                 5659 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013688
VERSION     V013688
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     RBS             3432..3454
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3537..4493
                     /gene="hly"
                     /label="Alpha-hemolysin"
                     /note="Alpha-hemolysin from Staphylococcus aureus (strain
                     NCTC 8325 / PS 47). Accession#: Q2G1X0"
     CDS             4530..5237
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      5304..5351
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     protein_bind    5385..5406
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5421..5451
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             5503..5520
                     /label="6xHis"
                     /note="6xHis affinity tag"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaatggt agcctttagt
     3361 ccatgttagc gaagaaaatg gtttgttata gtcgaataaa gagaccacaa cggtttccct
     3421 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaatac ctgctgccga
     3481 ccgctgctgc tggtctgctg ctcctcgctg cccagccggc gatggccatg gatatcatga
     3541 aaacacgtat agtcagctca gtaacaacaa cactattgct aggttccata ttaatgaatc
     3601 ctgtcgctaa tgccgcagat tctgatatta atattaaaac cggtactaca gatattggaa
     3661 gcaatactac agtaaaaaca ggtgatttag tcacttatga taaagaaaat ggcatgcaca
     3721 aaaaagtatt ttatagtttt atcgatgata aaaatcacaa taaaaaactg ctagttatta
     3781 gaacgaaagg taccattgct ggtcaatata gagtttatag cgaagaaggt gctaacaaaa
     3841 gtggtttagc ctggccttca gcctttaagg tacagttgca actacctgat aatgaagtag
     3901 ctcaaatatc tgattactat ccaagaaatt cgattgatac aaaagagtat atgagtactt
     3961 taacttatgg attcaacggt aatgttactg gtgatgatac aggaaaaatt ggcggcctta
     4021 ttggtgcaaa tgtttcgatt ggtcatacac tgaaatatgt tcaacctgat ttcaaaacaa
     4081 ttttagagag cccaactgat aaaaaagtag gctggaaagt gatatttaac aatatggtga
     4141 atcaaaattg gggaccatat gatagagatt cttggaaccc ggtatatggc aatcaacttt
     4201 tcatgaaaac tagaaatggt tctatgaaag cagcagataa cttccttgat cctaacaaag
     4261 caagttctct attatcttca gggttttcac cagacttcgc tacagttatt actatggata
     4321 gaaaagcatc caaacaacaa acaaatatag atgtaatata cgaacgagtt cgtgatgatt
     4381 accaattgca ttggacttca acaaattgga aaggtaccaa tactaaagat aaatggacag
     4441 atcgttcttc agaaagatat aaaatcgatt gggaaaaaga agaaatgaca aataagcttg
     4501 gcggcggagg gagtggagga gggggaagca tggtgagcaa gggcgaggag gataacatgg
     4561 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     4621 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4681 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     4741 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4801 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4861 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     4921 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4981 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     5041 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     5101 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     5161 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     5221 tggacgagct gtacaagtag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     5281 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     5341 ggggtttttt gctgaaagga ggaactatat ccggatgcgg ccgctaatgt gagttagctc
     5401 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatg
     5461 tctcatgagc ggatactttc acacaggaaa cagctactcg agcaccacca ccaccaccac
     5521 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     5581 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     5641 ggaggaacta tatccggat
//
