LOCUS       V014133                 5673 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014133
VERSION     V014133
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     RBS             3432..3454
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     3462..3527
                     /label="pelB signal sequence"
                     /note="leader peptide for secretion"
     CDS             3555..4511
                     /gene="hly"
                     /label="Alpha-hemolysin"
                     /note="Alpha-hemolysin from Staphylococcus aureus (strain
                     NCTC 8325 / PS 47). Accession#: Q2G1X0"
     CDS             4512..4538
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      4604..4651
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     protein_bind    4685..4706
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             4833..5507
                     /note="Light-activated DNA-binding protein EL222 from
                     Erythrobacter litoralis (strain HTCC2594). Accession#:
                     Q2NB98"
                     /label="Light-activated DNA-binding protein EL222"
     CDS             5517..5534
                     /label="6xHis"
                     /note="6xHis affinity tag"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaatggt agcctttagt
     3361 ccatgttagc gaagaaaatg gtttgttata gtcgaataaa gagaccacaa cggtttccct
     3421 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaatac ctgctgccga
     3481 ccgctgctgc tggtctgctg ctcctcgctg cccagccggc gatggccatg gatatcggaa
     3541 ttaattcgga tcctatgaaa acccgtatcg tttcttctgt taccaccacc ctgctgctgg
     3601 gttctatcct gatgaacccg gttgctaacg ctgctgactc tgacatcaac atcaaaaccg
     3661 gtaccaccga catcggttct aacaccaccg ttaaaaccgg tgacctggtt acctacgaca
     3721 aagaaaacgg tatgcacaaa aaagttttct actctttcat cgacgacaaa aaccacaaca
     3781 aaaaactgct ggttatccgt accaaaggta ccatcgctgg tcagtaccgt gtttactctg
     3841 aagaaggtgc taacaaatct ggtctggctt ggccgtctgc tttcaaagtt cagctgcagc
     3901 tgccggacaa cgaagttgct cagatctctg actactaccc gcgtaactct atcgacacca
     3961 aagaatacat gtctaccctg acctacggtt tcaacggtaa cgttaccggt gacgacaccg
     4021 gtaaaatcgg tggtctgatc ggtgctaacg tttctatcgg tcacaccctg aaatacgttc
     4081 agccggactt caaaaccatc ctggaatctc cgaccgacaa aaaagttggt tggaaagtta
     4141 tcttcaacaa catggttaac cagaactggg gtccgtacga ccgtgactct tggaacccgg
     4201 tttacggtaa ccagctgttc atgaaaaccc gtaacggttc tatgaaagct gctgacaact
     4261 tcctggaccc gaacaaagct tcttctctgc tgtcttctgg tttctctccg gacttcgcta
     4321 ccgttatcac catggaccgt aaagcttcta aacagcagac caacatcgac gttatctacg
     4381 aacgtgttcg tgacgactac cagctgcact ggacctctac caactggaaa ggtaccaaca
     4441 ccaaagacaa atggaccgac cgttcttctg aacgttacaa aatcgactgg gaaaaagaag
     4501 aaatgaccaa ctacccgtac gacgttccgg actacgctta gatccggctg ctaacaaagc
     4561 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4621 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatgcgg
     4681 ccgctaatgt gagttagctc actcattagg caccccaggc atttcctctt gtcaggccgg
     4741 aataactccc tataatgcgc atgctcgaaa tcgaaagagg agaaatgtgg aatgtctcat
     4801 gagcggatac tttcacacag gaaacagcta tgatgttgga tatgggacaa gatcggccga
     4861 tcgatggaag tggggcaccc ggggcagacg acacacgcgt tgaggtgcaa ccgccggcgc
     4921 agtgggtcct cgacctgatc gaggccagcc cgatcgcatc ggtcgtgtcc gatccgcgtc
     4981 tcgccgacaa tccgctgatc gccatcaacc aggccttcac cgacctgacc ggctattccg
     5041 aagaagaatg cgtcggccgc aattgccgat tcctggcagg ttccggcacc gagccgtggc
     5101 tgaccgacaa gatccgccaa ggcgtgcgcg agcacaagcc ggtgctggtc gagatcctga
     5161 actacaagaa ggacggcacg ccgttccgca atgccgtgct cgttgcaccg atctacgatg
     5221 acgacgacga gcttctctat ttcctcggca gccaggtcga agtcgacgac gaccagccca
     5281 acatgggcat ggcgcgccgc gaacgcgccg cggaaatgct caagacgctg tcgccgcgcc
     5341 agctcgaggt tacgacgctg gtggcatcgg gcttgcgcaa caaggaagtg gcggcccggc
     5401 tcggcctgtc ggagaaaacc gtcaagatgc accgcgggct ggtgatggaa aagctcaacc
     5461 tgaagaccag cgccgatctg gtgcgcattg ccgtcgaagc cggaatctga ctcgagcacc
     5521 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     5581 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     5641 gttttttgct gaaaggagga actatatccg gat
//
