LOCUS       V016076                 7623 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V016076
VERSION     V016076
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5334..7100
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      7173..7220
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7586..7614)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atggcatgga
     4081 aaccgaccga atatcagatt ggtgatcgtc atccggatat tgcagatgca aaagaacgtc
     4141 tgcgtcgtat tgcagcaaaa ctggttgcag gtcgtctgga taatgataat accgatgttt
     4201 ttagtccggt gtttgcagat gttctggcag aatggaaaac cgcagttcat cgtgatgttc
     4261 tgagcggtcg tcgtcagcct ccggatgttg atccgaccag caccgttatt gattgggcaa
     4321 ccaaagttca gctgggtatg attgcccgtg caacccctcc ggcaccggca ccgcctaaag
     4381 cacgtcatct gggtattgtt tttcgtggca ccggtggtat tattggtcag gattatgtta
     4441 gccgtgttct gcaggcatgt gcagatctgg ttgaagaagt tcatccggca tttgccgcaa
     4501 ccatgggtgg tattccggtt ggtacagcag gcggtattaa tgatccgagc atggcacatg
     4561 cagttgcact ggcatttgcg gatgcacagc gtatttttct ggaacgtttt cgtgcaaatc
     4621 cgcgtattcg tgttgttatt ggtggttatt cagccggtgc agttgccgca gcaatgtttc
     4681 gtgaatggct gctgaccaat tatccggaca attatctgtg tagctttagc tttggtgatc
     4741 cgacacgtcc ggcaggcggt gcatattttg gtggtgttgc agcacctggt cgtggtatta
     4801 gcacctggcg ttatggtgat attcgtgatt atcgtcattg ttggctggca gcaccgggtg
     4861 atatgtatac cagcgtgccg gataatgcag tgggcgacat tatggatacc gcctatgata
     4921 ttgttaccca ggttgaactg agcgattttc tgggcaccgc atttggtgtt gcccgtcaga
     4981 ttccgattat tatggaagaa gcaggtattg gtctgccgag cgtttttaaa gcactggctg
     5041 gcggtcctgc cggtctggca ggtctgggtg ttccgctgat tatgggtctg ttaggtggtc
     5101 tgattggtgg tcagaaaaat ccgaccggtg ttgcggcagc agcacaggca gcagttattg
     5161 cactgcagtt tgttaccagc aatccgccta ccgcagcaca tattcagtat gaatttcgtg
     5221 aagtttggcc tggccagacc tatctgggtt tagcaattca gcatgttcgc gattgggcag
     5281 gtcgtacacc ggcagttacc gcagttgatg caaatagcag tccgcgtcac atgatggcaa
     5341 ccggtaaagg tgcagcagca agcacccaag aaggtaaaag ccagccgttt aaagttacac
     5401 cgggtccgtt tgatccggca acctggctgg aatggtcacg tcagtggcag ggcaccgaag
     5461 gtaatggtca tgcagcagcc agcggtattc ctggtctgga tgccctggca ggcgttaaaa
     5521 ttgctcctgc acagctgggc gatattcagc agcgttatat gaaagatttt agcgcactgt
     5581 ggcaggcaat ggcggaaggt aaagcagaag caaccggtcc gctgcatgat cgtcgttttg
     5641 ccggtgatgc atggcgtacc aatctgccgt atcgttttgc agccgcattt tatctgctga
     5701 atgcacgtgc cctgaccgaa ctggcagatg ccgttgaagc cgatgcaaaa acccgtcagc
     5761 gtattcgctt tgcaattagc cagtgggttg atgccatgag tccggcaaat tttctggcaa
     5821 ccaatccgga agcgcagcgt ctgctgattg aaagcggtgg tgaaagcctg cgtgccggtg
     5881 ttcgtaatat gatggaagat ctgacccgtg gtaaaattag tcagaccgat gaaagcgcat
     5941 ttgaagttgg tcgtaatgtt gccgttaccg aaggtgccgt tgtttttgaa aatgaatatt
     6001 ttcagctgct gcagtacaaa ccgctgaccg ataaagttca tgcacgtcct ctgctgatgg
     6061 ttccgccttg tattaacaaa tattacatcc tggatctgca gccggaaagc agcctggttc
     6121 gtcatgttgt tgaacagggt cataccgttt ttctggttag ctggcgtaat ccggatgcaa
     6181 gcatggcagg tagtacctgg gatgattata ttgaacatgc agcaattcgt gccattgaag
     6241 ttgcacgtga tattagcggt caggataaaa tcaatgtgct gggtttttgt gttggtggca
     6301 ccattgttag caccgcactg gcagtgctgg cagcgcgtgg tgaacatccg gcagcaagcg
     6361 ttaccctgct gacaaccctg ctggattttg cagataccgg cattctggat gtttttgttg
     6421 atgaaggtca tgttcagctg cgtgaagcaa ccttaggtgg tggtgcgggt gcaccgtgtg
     6481 cactgctgcg tggtctggaa ctggcaaata cctttagttt tctgcgtccg aatgatctgg
     6541 tttggaatta tgtggttgac aactatctga aaggcaatac tccggttcct tttgatctgc
     6601 tgttttggaa tggtgatgca acaaatctgc caggtccgtg gtactgttgg tatctgcgtc
     6661 atacctatct gcagaatgaa ctgaaagttc cgggtaaact gaccgtttgc ggtgttccgg
     6721 ttgatctggc aagcattgat gttccgacct atatctatgg tagccgtgaa gatcatattg
     6781 ttccgtggac cgcagcctat gcaagtaccg cactgctggc caataaactg cgttttgttc
     6841 tgggtgcaag cggtcatatt gcgggtgtta ttaatcctcc ggcaaaaaac aaacgtagcc
     6901 attggaccaa tgatgcactg ccggaaagtc ctcagcagtg gttagcgggt gcaattgaac
     6961 atcatggtag ttggtggcca gattggaccg catggttagc aggtcaggca ggcgcaaaac
     7021 gtgcagcccc tgcaaattat ggtaatgcac gttatcgtgc gattgaacct gcaccgggtc
     7081 gttatgttaa agcaaaagca taactcgagg atccggctgc taacaaagcc cgaaaggaag
     7141 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     7201 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt
     7261 gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     7321 cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac
     7381 gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc
     7441 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     7501 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     7561 caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga
     7621 gaa
//
