LOCUS       V015402                 7359 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V015402
VERSION     V015402
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7359)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5070..6836
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      6909..6956
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7322..7350)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgtggattg
     4081 gttggcaaga aggtatgagc ggtcgtccgg ttctggcagc aaaaggtgaa ctgcgtgcaa
     4141 aatttagcta tggcaaaaat ctggatctga ccgaacattt tggtccggat ctgacagaag
     4201 cactgaaaac ctttcagcgt aataaaggtg gtctgcgtac cgatggtgtt ctggattatg
     4261 caacccagaa agcactgggt gtgctggaag ccctgaaacc gtgggttttt accgttgcag
     4321 gtacaggtgc aggttgggat gcaggttatc ctgccgaagt tgcacgtgcc gtgctggatc
     4381 tgtatcgttg gcagggtgtt aattatccgg caaaaccgtt tccgatgggt agcagcgttg
     4441 atgcaggtat tgcagaactg aaaaaactgc tgcgtgaacg tatggatcgc tatccggcag
     4501 cacgttttgt tctgattggt tatagccagg gtgcaattgt taccagcatg gtttggcaga
     4561 attttatcaa aggtagcgat ctggaagcgc gtattattgg tagcattacc tatggtaatc
     4621 cgtgtcgtga actgcatgtt gcaaatggta atgttgcaga aggtattccg gttccggaag
     4681 gtcgtggtat tagcgatttt cgtctgcgta gcaccccgag ttggtggtat gattttgcac
     4741 atggtgccaa tagccgtttt ggtcgtgata tctataccga tacaccggat gatgatgccg
     4801 gtgaaatgat gaccgcaatt tatcgtctgg ttcaggatct gaaaaacatt attgttggca
     4861 ccgatagcct gattgaacag gttggtgaaa tgtttcagcg tccgattacc gaagttggtg
     4921 cagcaatgtg ggcaattttt cttggtggtc agtttgttgc aacccgtccg tatccgacca
     4981 tgccgcatat taactatgat attaatccgg cagttgccat tctgcgtcgt attgccgcag
     5041 ttgatgcaaa tagcagtccg cgtcacatga tggcaaccgg taaaggtgca gcagcaagca
     5101 cccaagaagg caaaagccag ccgtttaaag ttacaccggg tccgtttgat ccggcaacct
     5161 ggctggaatg gtcacgtcag tggcagggca ccgaaggtaa tggtcatgca gcagccagcg
     5221 gtattcctgg tctggatgca ctggcaggcg ttaaaattgc accggcacag ctgggtgata
     5281 ttcagcagcg ttatatgaaa gattttagcg cactgtggca ggcaatggcg gaaggtaaag
     5341 cagaagcaac cggtccgctg catgatcgtc gttttgcggg tgatgcatgg cgtaccaatc
     5401 tgccgtatcg ttttgcagcc gcattttatc tgctgaatgc ccgtgcactg acagaactgg
     5461 cagatgcagt tgaagcagat gcaaaaaccc gtcagcgtat tcgctttgca attagccagt
     5521 gggttgatgc catgagtccg gcaaattttc tggccaccaa tccggaagca cagcgtctgc
     5581 tgattgaaag cggtggcgaa agcctgcgtg ccggtgttcg taatatgatg gaagatctga
     5641 cccgtggtaa aattagtcag accgatgaaa gcgcatttga agttggtcgt aatgtggcag
     5701 ttaccgaagg tgcagttgtt tttgaaaacg aatattttca gctgctgcag tataaaccgc
     5761 tgaccgataa agttcatgca cgtcctctgc tgatggttcc gccttgtatt aacaaatatt
     5821 acatcctgga tctgcagccg gaaagcagcc tggttcgtca tgttgttgaa cagggtcata
     5881 ccgtttttct ggttagctgg cgtaatccgg atgcaagcat ggcaggtagc acctgggatg
     5941 attatattga acatgcagca attcgtgcca ttgaagttgc ccgtgatatt agcggtcagg
     6001 ataaaatcaa tgtgctgggt ttttgtgttg gtggcaccat tgttagcacc gcactggcgg
     6061 tgctggcagc gcgtggtgaa catcctgcag caagcgttac cctgctgacc acactgctgg
     6121 attttgcaga taccggcatt ctggatgttt ttgttgatga aggtcatgtt cagctgcgcg
     6181 aagcaacctt aggtggtggt gcgggtgcac cgtgtgcact gttacgtggt ctggaactgg
     6241 caaatacctt tagctttctg cgtccgaatg atctggtttg gaattatgtg gttgacaact
     6301 atctgaaagg taatacaccg gtgccttttg atctgctgtt ttggaatggt gatgcaacaa
     6361 atctgcctgg tccgtggtat tgttggtatc tgcgtcatac ctatctgcag aatgaactga
     6421 aagttccggg taaactgacc gtttgtggtg ttccggttga tctggcaagc attgatgttc
     6481 cgacctatat ctatggtagc cgtgaagatc atattgttcc gtggaccgca gcctatgcaa
     6541 gtaccgcact gctggccaat aaactgcgct ttgttctggg tgcaagcggt catattgcgg
     6601 gtgttattaa tccaccggca aaaaacaaac gtagccattg gaccaatgat gcactgccgg
     6661 aaagtccgca gcagtggtta gccggtgcga ttgaacatca tggtagttgg tggcctgatt
     6721 ggaccgcatg gctggcaggt caggcaggcg caaaacgcgc agcccctgca aattatggta
     6781 atgcacgtta tcgtgcaatc gaaccggcac cgggtcgtta tgttaaagca aaagcataac
     6841 tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     6901 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6961 aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     7021 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     7081 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
     7141 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     7201 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     7261 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
     7321 cattaaagct tatcgatgat aagctgtcaa acatgagaa
//
