LOCUS       V015851                 7428 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V015851
VERSION     V015851
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5139..6905
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      6978..7025
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7391..7419)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgcgtattg
     4081 gtggtgaata tgttggttta ggtctgggtg atgcaagcga agaaattggt cgtattcgtg
     4141 attttatgcg tcgtaaattt cgtagctatg caggtaatct gccggataca cgtaatgcac
     4201 tgggtctgcc gctgtttgat gaaccgatga ccgttgcagt taccgaaatg cagggtcgtt
     4261 atctgcagag cggtgaactg cgtgatggtc tgtttatctt tggtattatc aacgccgaaa
     4321 ccaaatatgt gatgggttat ctggaacgtc cgcctggtcc ggatacgcgt ccgctgttta
     4381 ttaccgtttg tggcaccggt gttccgtggt gggttggtcc tgatgcagat accgcacgtg
     4441 catgtgaaga taaactgttt tggcagccga ttggttatcc ggcaaaaaac tttccgatgg
     4501 gtaaaagcat tgcagcaggt attgatcagt gtcatgttca gtttaatcgt gcagatccgg
     4561 gttttatgca tcgtcagcgt attgaacgta atggtgttgt tttaggtggt tatagccagg
     4621 gtgcagttgt tgttagcgaa ctgtgggaaa ataacattaa accggcaaat ggtagcctgc
     4681 attgggcaaa agattatgtt gttaaagcag tgacctgggg taatccgaat cgtgaagttg
     4741 gtgcagtttg gcctgattat ggtggtagcc cgatggcaag cctgaccagt cagggtgtta
     4801 gcagcaccgg tatgcgtgat accccgagtt ggtggcgtaa ttatgcacat gccggtgatc
     4861 tgtatgcagc agcagaaccg ggtgatagcc agcaggataa aaatgcaatt tggcagatta
     4921 ttcgcgatct gaactttttt accggcaccg atagcctgct ggcacaggca attgaactga
     4981 gccagatgcc gattccgcgt accattgcag cctttaaagc actgattgat gccggtatgt
     5041 tttttgcgaa aggcaccggt ccgcatgtgg attatggtat tggtccggct attgaatatc
     5101 tgcgtagcgt tgatgcaaat agcagtccgc gtcacatgat ggcaaccggt aaaggtgcag
     5161 ccgcaagcac ccaagaaggt aaaagccagc cgtttaaagt tacaccgggt ccgtttgatc
     5221 cggcaacctg gctggaatgg tcacgtcagt ggcagggcac cgaaggtaat ggtcatgccg
     5281 cagcaagcgg tattcctggt ctggatgcac tggcaggcgt taaaattgca ccggcacagc
     5341 tgggtgatat tcagcagcgt tatatgaaag attttagtgc actgtggcag gcaatggcgg
     5401 aaggtaaagc agaagcaaca ggtccgctgc atgatcgtcg ttttgcgggt gatgcctggc
     5461 gtaccaatct gccgtatcgt tttgcagcag ccttttatct gctgaatgcc cgtgcactga
     5521 ccgaactggc agatgcagtt gaagcagatg cgaaaacccg tcagcgcatt cgttttgcca
     5581 ttagccagtg ggttgatgcc atgagtccgg caaattttct ggcaaccaat ccggaagcac
     5641 agcgtctgct gattgaaagc ggtggtgaaa gcctgcgtgc cggtgttcgt aatatgatgg
     5701 aagatctgac ccgtggtaaa attagccaga ccgatgaaag cgcatttgaa gtgggtcgta
     5761 atgttgccgt gaccgaaggt gccgttgttt ttgaaaatga atattttcag ctgctgcagt
     5821 ataaaccgct gaccgataaa gttcatgcac gtcctctgct gatggttccg ccttgtatta
     5881 acaaatatta catcctggat ctgcagccgg aaagcagcct ggttcgtcat gttgttgaac
     5941 agggtcatac cgtttttctg gttagctggc gtaatccgga tgcaagcatg gcaggtagca
     6001 cctgggatga ttatattgaa catgcagcaa ttcgtgccat tgaagttgca cgtgatatta
     6061 gcggtcagga caaaattaac gttctgggtt tttgtgttgg tggcaccatt gttagcaccg
     6121 cactggccgt tctggcagca cgtggtgaac atccggcagc cagcgttacc ctgctgacca
     6181 cactgctgga ttttgcagat acaggtattc tggatgtgtt tgtggatgaa ggtcatgtgc
     6241 agctgcgtga agcaacctta ggtggtggtg cgggtgcacc gtgtgcactg ctgcgtggtc
     6301 tggaactggc aaataccttt agctttctgc gtccgaatga tctggtttgg aattatgtgg
     6361 ttgacaacta tctgaaaggt aatactccgg ttccatttga tctgctgttt tggaatggtg
     6421 atgccacaaa tctgccaggt ccgtggtatt gttggtatct gcgtcatacc tacctgcaga
     6481 atgaactgaa agttccgggt aaactgaccg tgtgtggtgt tccggttgat ctggcaagca
     6541 ttgatgttcc gacctatatc tatggtagcc gtgaagatca tattgtgccg tggaccgcag
     6601 cctatgcaag taccgcactg ctggccaata aactgcgttt tgttctgggt gcaagcggtc
     6661 atattgcggg tgttattaat ccgcctgcca aaaacaaacg tagccattgg accaatgatg
     6721 cactgccgga aagtcctcag cagtggttag cgggtgcgat tgaacatcat ggttcatggt
     6781 ggccagattg gaccgcatgg ctggcaggtc aggcaggcgc aaaacgtgcc gcaccggcta
     6841 attatggtaa tgcacgttat cgtgctattg aaccggctcc gggtcgctat gttaaagcca
     6901 aagcataact cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     6961 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     7021 ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc
     7081 gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg
     7141 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc
     7201 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc
     7261 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
     7321 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
     7381 aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
//
