LOCUS       V015650                 7245 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V015650
VERSION     V015650
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4956..6722
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      6795..6842
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7208..7236)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgaccacct
     4081 atggtgaact gaaagcactg cgtctgggtg ttaaatatgt tcgtcatacc ctgtttaccg
     4141 ttgcaggcac ctgggcagat atgtggtcag gttatccggc agatgttgca cgtctggttg
     4201 atgaagattt atttcgttgg cagccggttt ggtatccggc aagctttggt ccggttggta
     4261 atccgctggg tcgtagctat caagaaagcg ttcaggatgg tgttaaagaa ctgattcgtc
     4321 tgattaatgc aacaccgggt acatttgcac tggttggtta tagccagggt gccgaagttg
     4381 ttagccgtgt tctgctggaa attctgtttg gtagcctgcg tcatcgtctg aaagatttca
     4441 ttggtggtgg ttgttttggc aatccgtatc gtgcaaaagg tgttagctat cctggtagcg
     4501 gtctgccgac cagcggtcat ggtattgcac cggttaatct ggcaccggat attctgcctg
     4561 cagaaatgtg ggaagaatgg tggaatgaag gtgatctgta tgcacagaat ctggatggta
     4621 aaagcggtga aatcatcacc agcttttatg acattctgac caagctgcag tttcatgata
     4681 tgctgggttt agccgtgaat atgtttaaag cactgagcaa cgataaaggc attattgccc
     4741 aggttatgcg tgttctggca gttccgctgc ctggtgttat tgatgcaggt cgtgcagcag
     4801 tttatgcagg tacgtttgca gttcagggca cccgtccgca tattaccctg gcagaaaccg
     4861 gtcgtgttgc ccgtgcagtt tggcatctga atcgtattgg tgcaaaaacc ctggcacgtg
     4921 caagcgttga tgcaaatagc agtccgcgtc acatgatggc aaccggtaaa ggtgcagcag
     4981 caagcaccca agaaggtaaa agccagccgt ttaaagttac accgggtccg tttgatccgg
     5041 caacctggct ggaatggtca cgtcagtggc agggcaccga aggtaatggt catgcagcag
     5101 ccagcggtat tcctggtctg gatgcactgg caggcgttaa aatcgcaccg gcacagctgg
     5161 gtgatattca gcagcgttat atgaaagact ttagcgcact gtggcaggca atggcggaag
     5221 gtaaagcaga agcaaccggt ccgctgcatg atcgtcgttt tgccggtgat gcatggcgta
     5281 ccaatctgcc gtatcgtttt gcagccgcat tttatctgct gaatgcacgt gcgctgaccg
     5341 aactggcaga tgcagttgaa gcagatgcga aaacccgtca gcgtattcgc tttgcaatta
     5401 gccagtgggt tgatgccatg agtccggcaa attttctggc aaccaatccg gaagcgcagc
     5461 gtctgctgat tgaaagcggt ggtgaaagtc tgcgtgccgg tgttcgtaat atgatggaag
     5521 atctgacccg tggtaaaatt agtcagaccg atgaaagcgc atttgaagtt ggtcgtaatg
     5581 ttgcagttac cgaaggtgca gttgtgtttg aaaacgaata ttttcagctg ctgcagtata
     5641 aaccgctgac agataaagtt catgcccgtc ctctgctgat ggttccgcct tgtattaaca
     5701 aatattacat cctggatctg cagccggaaa gcagcctggt tcgtcatgtt gttgaacagg
     5761 gtcataccgt ttttctggtt agctggcgta atccggatgc aagcatggca ggtagcacct
     5821 gggatgatta tattgaacat gcagcaattc gtgccattga agttgcacgt gatattagcg
     5881 gtcaggataa aatcaatgtt ctgggttttt gtgttggtgg caccattgtt agcaccgcac
     5941 tggccgtgct ggcagcccgt ggtgaacatc cggcagcaag cgttaccctg ctgaccacac
     6001 tgctggattt tgcagatacc ggcattctgg atgtttttgt ggatgaaggt catgttcagc
     6061 tgcgtgaagc aaccttaggt ggtggtgcgg gtgcaccgtg tgcactgctg cgtggtctgg
     6121 aactggcaaa tacctttagc tttctgcgtc cgaatgatct ggtttggaat tatgtggttg
     6181 acaactatct gaaaggtaat acaccggtgc cttttgatct gctgttttgg aatggtgatg
     6241 caacaaatct gcctggtccg tggtattgtt ggtatctgcg tcatacctat ctgcagaatg
     6301 agctgaaagt tccgggtaaa ctgaccgttt gtggtgttcc ggtggatctg gcaagcattg
     6361 atgttccgac ctatatctat ggtagtcgcg aagatcatat tgttccgtgg accgcagcct
     6421 atgcaagtac cgcactgctg gccaataaac tgcgttttgt gctgggtgca agcggtcata
     6481 ttgcgggtgt tattaatccg cctgcaaaaa acaaacgtag ccattggacc aatgatgcac
     6541 tgccggaaag tcctcagcag tggttagcgg gtgcaattga acatcatggt agttggtggc
     6601 ctgattggac cgcatggctg gcaggtcagg caggcgcaaa acgtgcagcc cctgcaaatt
     6661 atggtaatgc acgttatcgt gcgattgaac cggcaccggg tcgttatgtt aaagcaaaag
     6721 cataactcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     6781 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     6841 tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg
     6901 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
     6961 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     7021 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     7081 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     7141 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     7201 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
//
