LOCUS       V016474                 7260 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V016474
VERSION     V016474
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4971..6737
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      6810..6857
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7223..7251)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgagcaaac
     4081 cgatgctgct gaccgcagca ggcaccaaag cagatgaatg gaccggttat ccggcagatc
     4141 tggcacgtcg tatggaagat ctgtattatt tccagccggt tcgttatggt ccgaatggta
     4201 ttccggcaat gtggccgatg ggtgcaagcg caaaaagcgg tattgatgaa ggtgttcgtc
     4261 tggttctgga agccgaagca cgtccgagcc gtgaagttcc ggatggttat gcagtttgtg
     4321 gttatagcca aggtggttgg gttgttagcg aactgctggg tgaatttcgt accggtcgtc
     4381 tgaaacatct gcgtgataaa ctgatggcag gcgcaacctt tggtaatccg tatcgtgaac
     4441 tggatagtga tggtggtcgt ggtattagcg ataaacgtat tgttgataca ccggattttt
     4501 gggtcgatga atttgatccg ggtgatattt atgccaatgt gccgaataat gatgtgggtg
     4561 aagatatgac cgcaatcttt aaactggttc gctttagtgg tattggcgac gttattgatc
     4621 tgggtagcgc aatcgatctg ggttcaattg ccggtggtct gctgcctggt ggtggtcagt
     4681 taggtggtat tttaggtggt ttaggcggtc tgcttggtgg cggtgcacgt cagcaggata
     4741 atattgttga gcagattgtt gaaatgctgc gtagtccgct gcgcgaattt ccggcagcag
     4801 ttagcgcaat tctgaaaggt ctggtttttg ttggtcagaa accggcaacc gcaccgcata
     4861 ttgaatatca tctgcgcgaa cgtagtccgg gtgttaccta ttatgaacat gcagttgcac
     4921 acatgagagc aatggcagcc gttgatgcaa atagcagtcc gcgtcacatg atggcaaccg
     4981 gtaaaggtgc agcagcaagc acccaagaag gtaaaagcca gccgtttaaa gttacaccgg
     5041 gtccgtttga tcctgcaacc tggctggaat ggtcacgtca gtggcagggc accgaaggta
     5101 atggtcatgc agcagccagc ggtattcctg gtctggatgc actggcaggc gttaaaattg
     5161 caccggcaca gctgggcgat attcagcagc gttatatgaa agattttagc gcactgtggc
     5221 aggcaatggc ggaaggtaaa gcagaagcaa ccggtccgct gcatgatcgt cgttttgccg
     5281 gtgatgcatg gcgtaccaat ctgccgtatc gttttgcagc cgcattttat ctgctgaatg
     5341 cacgtgccct gaccgaactg gcagatgcag ttgaagcaga tgcaaaaacc cgtcagcgta
     5401 ttcgctttgc aattagccag tgggttgatg ccatgagtcc ggcaaatttt ctggcaacca
     5461 atccggaagc acagcgtctg ctgattgaaa gcggtggtga aagcctgcgt gccggtgttc
     5521 gtaatatgat ggaagattta acccgtggta aaatcagcca gaccgatgaa agcgcatttg
     5581 aagttggtcg taatgttgca gttaccgaag gtgcagttgt gtttgaaaac gaatattttc
     5641 agctgctgca gtataaaccg ctgaccgata aagttcatgc acgtcctctg ctgatggttc
     5701 cgccttgtat taacaaatat tacatcctgg atctgcagcc ggaaagcagc ctggttcgtc
     5761 atgttgttga acagggtcat accgtttttc tggttagctg gcgtaatccg gatgcaagca
     5821 tggcaggtag cacctgggat gattatattg aacatgccgc aattcgtgca attgaagttg
     5881 cacgtgatat tagcggtcag gataaaatca atgtgctggg tttttgtgtt ggtggcacca
     5941 ttgttagcac cgcactggcc gttctggcag cacgtggtga acatccggca gcgagcgtta
     6001 ccctgctgac cacactgctg gattttgcag ataccggcat tctggatgtt tttgtggatg
     6061 aaggtcatgt tcagctgcgt gaagcaacct taggtggtgg tgcgggtgca ccgtgtgcac
     6121 tgctgcgtgg tctggaactg gcaaatacct ttagctttct gcgtccgaat gatctggttt
     6181 ggaattatgt ggttgacaac tatctgaaag gcaatacacc ggtgcctttt gatctgctgt
     6241 tttggaatgg tgatgcaaca aatctgcctg gtccttggta ttgttggtat ctgcgtcata
     6301 cctatctgca gaatgaactg aaagttccgg gtaaactgac cgtttgtggt gttccggttg
     6361 atctggcaag cattgatgtt ccgacctata tctatggtag tcgcgaagat catattgttc
     6421 cgtggaccgc agcctatgca agtaccgcac tgctggccaa taaactgcgt tttgttctgg
     6481 gtgcatcagg tcatattgcg ggtgttatta atccgcctgc aaaaaacaaa cgtagccatt
     6541 ggaccaatga tgcactgccg gaatcacctc agcagtggtt agccggtgcc attgaacatc
     6601 atggtagttg gtggcctgat tggaccgcat ggctggcagg tcaagccggt gcgaaacgtg
     6661 cagcccctgc aaattatggt aatgcacgtt atcgtgcgat tgaaccggca ccgggtcgtt
     6721 atgttaaagc aaaagcataa ctcgaggatc cggctgctaa caaagcccga aaggaagctg
     6781 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     6841 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg
     6901 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
     6961 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
     7021 tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc
     7081 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
     7141 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
     7201 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
//
