LOCUS       V016461                 7152 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V016461
VERSION     V016461
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4071..4832
                     /gene="12"
                     /label="Endolysin B"
                     /note="Endolysin B from Mycobacterium phage D29.
                     Accession#: O64205"
     CDS             4863..6629
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      6702..6749
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7115..7143)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgagcaaac
     4081 cgtggctgtt taccgttcat ggcaccggtc agcctgatcc gttaggtccg ggtctgcctg
     4141 cagataccgc acgtgatgtt ctggatattt atcgttggca gccgattggt aattatccgg
     4201 cagcagcatt tccgatgtgg ccgagcgttg aaaaaggtgt tgcagaactg attctgcaga
     4261 ttgaactgaa actggatgca gatccgtatg cagattttgc aatggcaggt tatagccagg
     4321 gtgcaattgt tgttggtcag gttctgaaac atcatattct gcctccgaca ggtcgtctgc
     4381 atcgttttct gcaccgtctg aaaaaagtga ttttttgggg taatccgatg cgccagaaag
     4441 gttttgcaca tagtgatgaa tggattcatc cggttgcagc accggataca ctgggtattc
     4501 tggaagatcg tctggaaaat ctggaacagt atggttttga agttcgcgat tatgcacatg
     4561 atggtgatat gtatgccagc atcaaagaag atgatctgca cgaatatgaa gttgccattg
     4621 gtcgtattgt tatgaaagcc agcggtttta ttggtggtcg tgatagcgtt gttgcacagc
     4681 tgatcgaact gggtcagcgt ccgattaccg aaggtattgc actggcaggc gcaattattg
     4741 atgcactgac cttttttgca cgtagccgta tgggtgataa atggcctcat ctgtataatc
     4801 gttatcctgc cgttgaattt ctgcgtcaga ttgttgatgc aaatagcagt ccgcgtcaca
     4861 tgatggcaac cggtaaaggt gcagcagcaa gcacccaaga aggtaaaagt cagccgttta
     4921 aagttacacc gggtccgttt gatccggcaa cctggctgga atggtcacgt cagtggcagg
     4981 gcaccgaagg caatggtcat gcagccgcaa gcggtattcc tggtctggat gcattagccg
     5041 gtgttaaaat tgcaccggca cagctgggtg atattcagca gcgttatatg aaagatttta
     5101 gcgcactgtg gcaggcaatg gccgaaggta aagcagaagc aaccggtccg ctgcatgatc
     5161 gtcgttttgc cggtgatgca tggcgtacca atctgccgta tcgttttgca gcagcctttt
     5221 atctgctgaa tgcacgtgcc ctgaccgaac tggcagatgc agttgaagca gatgcaaaaa
     5281 cccgtcagcg tattcgcttt gcaattagcc agtgggttga tgccatgagt ccggcaaatt
     5341 ttctggcaac caatccggaa gcacagcgtc tgctgattga aagcggtggt gaaagcctgc
     5401 gtgccggtgt tcgtaatatg atggaagatc tgacccgtgg taaaattagt cagaccgatg
     5461 aaagcgcatt tgaagttggt cgtaatgtgg ccgtgaccga aggtgcagtt gtttttgaaa
     5521 atgaatattt ccagctgctg cagtataaac cgctgaccga taaagttcat gcacgtcctc
     5581 tgctgatggt tccgccttgt attaacaaat attacatcct ggatctgcag ccggaaagca
     5641 gcctggttcg tcatgttgtt gaacagggtc ataccgtttt tctggttagc tggcgtaatc
     5701 cggatgcaag catggcaggt agcacctggg atgattatat tgaacatgca gcaattcgtg
     5761 ccattgaagt tgcacgtgat attagcggtc aggataaaat caatgtgctg ggtttttgtg
     5821 ttggtggcac cattgttagc accgcactgg ccgttctggc agcacgtggt gaacatcctg
     5881 cagcaagcgt taccctgctg accacactgc tggattttgc cgataccggc attctggatg
     5941 tttttgttga tgaaggtcat gttcagctgc gtgaagcaac cttaggtggt ggtgcgggtg
     6001 caccgtgtgc actgctgcgt ggtctggaac tggcaaatac ctttagcttc ctgcgtccga
     6061 atgatctggt ttggaattat gtggttgaca actatctgaa aggtaataca ccggtgccgt
     6121 tcgacctgct gttttggaat ggtgatgcaa caaatctgcc tggtccgtgg tattgttggt
     6181 atctgcgtca tacctatctg caaaatgagc tgaaagttcc gggtaaactg accgtttgtg
     6241 gtgttccggt tgatctggca agcattgatg ttccgaccta tatctatggt agccgtgaag
     6301 atcatattgt tccgtggacc gcagcctatg caagtaccgc actgctggcc aataaactgc
     6361 gttttgttct gggtgcaagc ggtcatattg cgggtgttat taatccgcct gcaaaaaaca
     6421 aacgtagcca ttggaccaat gatgccctgc cggaaagtcc tcagcagtgg ttagcgggtg
     6481 cgattgaaca tcatggtagt tggtggcctg attggaccgc atggctggca ggtcaagcgg
     6541 gtgcaaaacg tgcagcccct gcaaattatg gtaatgcacg ttatcgtgca attgaacctg
     6601 caccgggtcg ttatgttaaa gcaaaagcat aactcgagga tccggctgct aacaaagccc
     6661 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     6721 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
     6781 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     6841 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     6901 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     6961 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     7021 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     7081 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     7141 caaacatgag aa
//
