LOCUS       V015270                 7896 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V015270
VERSION     V015270
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5145..5156
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             5607..7373
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      7446..7493
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7859..7887)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgaccgaaa
     4081 ttgttctgcc gtatgaccgt gcagttgttc cgcaagaaac cggttattgg tgtggtccgg
     4141 cagcaaccca ggttgttctg aatagccgtg gtattatcaa agcagaaagc gatctggcac
     4201 gtcagattgg caccaccaca cgtggcaccg attatgttgg tctgattgaa cgtgttctgg
     4261 atcagattgt tccggatgca cgttatacca gcgtgtatat tgaaaatgat ccgcctaccg
     4321 cagcacagaa agaaaccctg tggcgtaatc tggttgcaag tattaatgca ggttatggcg
     4381 ttgtgatgaa ttgggttgca ccgcctagca ataaaccgcg tggtgttaaa ggtagcgcaa
     4441 gtccggttta tagcggtggt acaacctatc attatgttgc agcaatgggc tatgatgata
     4501 atccggcagc gcgtgcagtt tggattgcag atagcggttt tcgtccgttt ggttattggg
     4561 ttagctttga tcagtgtgca accctgattc cgcctaaagg ttattgttat gcagcagcag
     4621 cccctgcagc accggcaccg cctgcaccag cacctccagc agcagttgat ctggtggcaa
     4681 ttctggcaca ggcaatgagc ccgaccaccg ttagccgtga taccctggca gcatatctgc
     4741 cgcattttgc cgaagcaatg cgtgcagcag atattaccac cgttcgtcgt gcagccgcat
     4801 ggtgtagcca ggttggtcat gaaagcgcag gtctgcgtta tatggcagaa attcagaccg
     4861 atggtcctgg ttggaccgaa gatcgtaaac gttatcgtgg tcgtggtccg attcagctga
     4921 cctggtcaag caattatcgc aaatttggtc agtggtgtgc agcacgtggt tatgttaccg
     4981 atccggaact gtttgttaat cagccggaac tggttgaaca gcctcgttgg ggttttctgg
     5041 cagcaagctg gtattggctg tttggtggtc cgaaaccggg tcagattaat gcctatgcag
     5101 atgccggtga tattctggca gttagccgtt gtgttaatgg ttggattgaa ggtcgtaatc
     5161 cggttggttg gcctgatcgt caagaacgtt ggaatcgttg tctggcactg ggtgatcagc
     5221 tgctgaccct gaccaccagt accacaccga ccgatccgat tgaagaactg ctgatgagca
     5281 atctgcgtgt tccgagcatg agcatttatg caacaccggg tgaaccggat gttccgattg
     5341 ttgatatgat tcgtgcactg gatgcacatg gtgatcatga aagttatgtt gaacgtcagg
     5401 cactgctggg tgataccgat gcaattgccc gtattgttcg taccgcagcc ggtaaaggta
     5461 aatatggtaa tgcaaccggt ccggttaatc aggcaaaaaa tgcactgaaa caaatcgaag
     5521 cagttaatcc tgctgcgctg aaacagtttc tggataatca gaaaggtagc attgcagttg
     5581 atgcaaatag cagtccgcgt cacatgatgg caaccggcaa aggtgccgca gcaagcaccc
     5641 aagaaggtaa aagccagccg tttaaagtta caccgggtcc gtttgatccg gcaacctggt
     5701 tagaatggtc acgtcagtgg cagggcaccg aaggtaatgg tcatgcagcg gcaagcggta
     5761 ttcctggtct ggatgccctg gcaggcgtta aaattgctcc ggcacagctg ggcgatattc
     5821 agcagcgcta tatgaaagat tttagcgcac tgtggcaggc aatggcggaa ggtaaagcag
     5881 aagccaccgg tcctctgcat gatcgtcgtt ttgcgggtga tgcatggcgt accaacctgc
     5941 cgtatcgttt tgcagcagcc ttttatctgc tgaatgcacg tgccctgacc gaactggcag
     6001 atgcagttga agcagatgca aaaacccgtc agcgtattcg ctttgcaatt agccagtggg
     6061 ttgatgccat gagtccggca aattttctgg ccaccaatcc ggaagcacag cgtctgctga
     6121 ttgaaagcgg tggtgaaagc ctgcgtgccg gtgttcgtaa tatgatggaa gatctgacac
     6181 gtggtaaaat cagccagaca gatgaaagtg catttgaagt tggtcgtaat gttgcagtta
     6241 ccgaaggtgc cgttgttttt gaaaacgaat atttccagct gctgcagtat aaaccgctga
     6301 ccgataaagt tcatgcacgt ccgctgctga tggttccgcc ttgtattaac aaatattaca
     6361 tcctggatct gcagccggaa agcagcctgg ttcgtcatgt tgttgaacag ggtcataccg
     6421 tttttctggt tagctggcgc aatccggatg ccagcatggc aggtagcacc tgggatgatt
     6481 atattgaaca tgcagcaatt cgtgccattg aagttgcacg tgatattagc ggtcaggata
     6541 aaatcaatgt gctgggtttt tgtgttggtg gcaccattgt tagcaccgca ctggcagtgc
     6601 tggcagcccg tggtgaacat cctgcagcaa gcgttaccct gctgaccaca ctgctggatt
     6661 ttgcagatac cggcattctg gatgtttttg ttgatgaagg tcatgttcag ctgcgtgaag
     6721 caaccttagg cggtggtgcg ggtgcaccgt gtgcactgct gcgtggtctg gaactggcaa
     6781 atacctttag ctttctgcgt ccgaatgatc tggtttggaa ttatgtggtt gacaactatc
     6841 tgaaaggtaa tacaccggtg ccatttgatc tgctgttttg gaatggtgat gcgaccaatc
     6901 tgcctggtcc ttggtattgt tggtatctgc gtcataccta tctgcagaat gaactgaaag
     6961 ttccgggtaa actgaccgtt tgtggtgttc cggtggatct ggcaagcatt gatgtgccga
     7021 cctatatcta tggtagccgt gaagatcata ttgtgccgtg gaccgcagcc tatgcaagta
     7081 cagccctgct ggccaataaa ctgcgttttg ttctgggtgc aagcggtcat attgcgggtg
     7141 ttattaatcc gcctgcaaaa aacaaacgta gccattggac caatgatgca ctgccggaaa
     7201 gtcctcagca atggttagcc ggtgcaattg aacatcatgg ttcatggtgg cctgattgga
     7261 ccgcatggct ggcaggtcag gcaggcgcaa aacgtgccgc accggcaaac tatggcaatg
     7321 cacgctatcg tgcgattgaa cctgcaccgg gtcgttatgt taaagcaaaa gcataactcg
     7381 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     7441 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     7501 gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
     7561 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     7621 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
     7681 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
     7741 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     7801 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
     7861 taaagcttat cgatgataag ctgtcaaaca tgagaa
//
