LOCUS       V015571                 7869 bp    DNA     circular SYN 20-APR-2021
DEFINITION  Exported.
ACCESSION   V015571
VERSION     V015571
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Davies CG, Reilly K, Altermann E, Hendrickson HL.
  TITLE     PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Q12
            Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Hendrickson HL, Davies CG, Altermann E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) School of Natural and Computational
            Sciences, Massey University, Private Bag 102904, Auckland 0745, New
            Zealand
REFERENCE   3  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-OCT-2020) School of Natural and Computational Sciences, Massey
            University, Private Bag 102904, Auckland 0745, New Zealand"
FEATURES             Location/Qualifiers
     source          1..7869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2014..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        4010..4028
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4071..5549
                     /gene="10"
                     /label="Endolysin A"
                     /note="Endolysin A from Mycobacterium phage D29.
                     Accession#: O64203"
     CDS             5580..7346
                     /gene="phaC"
                     /label="Poly(3-hydroxyalkanoate) polymerase subunit PhaC"
                     /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaC
                     from Cupriavidus necator (strain ATCC 17699 / DSM 428 /
                     KCTC 22496 / NCIMB 10442 / H16 / Stanier 337). Accession#:
                     P23608"
     terminator      7419..7466
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7832..7860)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agaaataagg agatatacat atgaccctga
     4081 ttgttacccg tgaacatgca cagtgggttc atgatatgtg tcgtgcacgt gccggtaatc
     4141 gttatggtta tggtggtgca tttaccctga atccgcgtga taccaccgat tgtagcggtc
     4201 tggttctgca gaccgcagcc tggtatggtg gtcgtaaaga ttgggttggc aatcgctatg
     4261 gtagcaccga aagttttcgt ctggatcaca aaattgtgta tgacctgggt tttaaacgtc
     4321 tgcctcctgg tggtgttgca gcactgggct ttacaccggt tatgctggtt ggtctgcagc
     4381 atggtggtgg tggccgttat agccataccg catgtaccct gatgaccatg gatattccag
     4441 gtggtccggt taaagttagc cagcgtggtg ttgattggga aagccgtggt gaagttaatg
     4501 gtgttggtgt ttttctgtat gatggtgccc gtgcatggaa tgatccgctg tttcatgatt
     4561 tttggtatct ggatgcaaag ctggaagatg gtccgacaca gagcgttgat gcagcagaaa
     4621 ttctggcacg tgcgaccggt ctggcatata atcgtgcagt tgcactgctg cctgcagttc
     4681 gtgatggtct gattcaggca gattgtacca atccgaatcg tattgcaatg tggctggcac
     4741 agattggtca tgaaagtgat gattttaaag ccaccgcaga atatgcaagc ggtgatgcct
     4801 atgatacccg taccgatctg ggtaatacac cggaagtgga tggtgatggt cgtctgtata
     4861 aaggtcgtag ctggattatg attacgggca aagataacta tcgtgatttt agccgttggg
     4921 cacatggtcg tggtctggtg ccgacaccgg attattttgt tgttcatccg ctggaactga
     4981 gcgaactgcg ttgggcaggt attggtgccg catggtattg gaccgttgaa cgtccggata
     5041 tcaatgcact gagcgatcgt cgtgatctgg aaaccgttac acgtcgtatt aatggtggtc
     5101 tgacaaatct ggatgatcgt cgtcgtcgct ataatctggc actggcagtt ggtgatcagc
     5161 tgctgacact gattggtgat gatgatgaac tggcagatcc gaccattcag cgttttattc
     5221 gtgaaattca tggtgccctg tttaataccg ttgttaccca gagtccgtat ggtgatccgc
     5281 agaatccgga tggtagcgaa ccgcgtagca acctgtggca gctgcacgaa ctgattaaaa
     5341 acggtgatgg tatgggtcat gcacgttatg ttgaagaaag cgcacgtgca ggcgatctgc
     5401 gtgaactgga acgtgttgtt cgtgccgcaa aaggtctggg tcgtgatcgt agtccggaat
     5461 ttattgcgcg tgcacgtaat gttctggccc agattgaagc agcaaatccg gaatatctgc
     5521 aggcatatat tgcacgcaat ggtgcactgg ttgatgcaaa tagcagtccg cgtcacatga
     5581 tggcaaccgg taaaggtgca gcagcaagca cccaagaagg taaaagccag ccgtttaaag
     5641 ttacaccggg tccgtttgat ccggcaacct ggctggaatg gtcacgtcag tggcagggca
     5701 ccgaaggtaa tggtcatgca gcagccagcg gtattcctgg tctggatgcc ctggcaggcg
     5761 ttaaaattgc accggcacag ctgggtgata ttcagcagcg ttatatgaaa gattttagcg
     5821 cactgtggca ggcaatggcg gaaggtaaag cagaagcaac cggtccgctg catgatcgcc
     5881 gttttgccgg tgatgcatgg cgtaccaatc tgccgtatcg ttttgcagcc gcattttatc
     5941 tgctgaatgc ccgtgcgctg accgaactgg ccgatgcagt tgaagcagat gcaaaaaccc
     6001 gtcagcgtat tcgctttgca attagccagt gggttgatgc catgagtccg gcaaattttc
     6061 tggcaaccaa tccggaagca cagcgtctgc tgattgaaag tggtggtgaa agcctgcgtg
     6121 ccggtgttcg taatatgatg gaagatctga cccgtggtaa aattagtcag accgatgaaa
     6181 gcgcatttga agttggtcgt aatgttgcag ttaccgaagg tgcagttgtg tttgaaaacg
     6241 aatatttcca gctgctgcag tataaaccgc tgacagataa agttcatgcg cgtcctctgc
     6301 tgatggttcc gccttgtatt aacaaatatt acatcctgga tctgcagccg gaaagcagtc
     6361 tggttcgtca tgttgttgaa cagggccata ccgtgtttct ggttagctgg cgtaacccgg
     6421 atgcaagcat ggcaggtagc acctgggatg attatattga acatgccgca attcgtgcaa
     6481 ttgaagttgc acgtgatatt agcggtcagg ataaaatcaa tgtgctgggt ttttgtgttg
     6541 gtggcaccat tgttagcacc gcactggccg ttctggcagc acgtggtgaa catccggcag
     6601 caagcgttac cctgctgaca accctgctgg attttgcaga taccggcatt ctggatgttt
     6661 ttgttgatga aggtcatgtt cagctgcgtg aagcaacctt aggtggcggt gcgggtgcac
     6721 cgtgtgcgct gctgcgtggt ttagaactgg caaatacctt tagctttctg cgtccgaatg
     6781 atctggtttg gaattatgtg gttgacaact atctgaaagg taatactccg gttccttttg
     6841 atctgctgtt ttggaatggt gatgcaacaa atctgccagg tccgtggtat tgctggtatc
     6901 tgcgtcatac ctatctgcag aatgaactga aagttccggg taaactgacc gtttgtggtg
     6961 ttccggttga tctggcaagc attgatgttc cgacctatat ctatggcagc cgtgaagatc
     7021 atattgttcc gtggaccgca gcgtatgcaa gtaccgcact gctggccaat aaactgcgtt
     7081 ttgttctggg tgccagcggt catattgcgg gtgttattaa tccgcctgca aaaaacaaac
     7141 gtagccattg gaccaatgat gcactgccgg aaagtccgca gcaatggtta gccggtgcca
     7201 ttgaacatca tggtagttgg tggcctgatt ggaccgcatg gctggcaggt caggcaggcg
     7261 caaaacgtgc agcccctgca aattatggta atgcccgtta tcgtgcgatt gaaccggcac
     7321 cgggtcgtta tgttaaagca aaagcataac tcgaggatcc ggctgctaac aaagcccgaa
     7381 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     7441 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg
     7501 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
     7561 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
     7621 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
     7681 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
     7741 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
     7801 cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa
     7861 acatgagaa
//
