LOCUS       40924_18261        4220 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pET-SP6p(-6T)-TetO-GFP-LVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     A unified design space of synthetic stripe-forming networks
  JOURNAL   Nat Commun 5, 4905 (2014)
  PUBMED    25247316
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2014) Centre for Genomic Regulation (CRG), 
            EMBL/CRG Systems Biology Research Unit, Dr. Aiguader 88, Barcelona, 
            Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 4220)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4220)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 4905 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUL-2014) Centre for Genomic Regulation (CRG), EMBL/CRG Systems 
            Biology Research Unit, Dr. Aiguader 88, Barcelona, Barcelona 08003, 
            Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             495..683
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    788..930
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1116..1704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1878..2735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2736..2840)
                     /label=AmpR promoter
     primer_bind     2880..2896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2909..2927
                     /gene="gfp-LVA"
                     /label=SP6(-6T) promoter
                     /note="SP6(-6T) promoter"
                     /regulatory_class="promoter"
     protein_bind    2932..2950
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             3058..3080
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3088..3801
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     terminator      3951..3998
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4007..4093
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4185..4212
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 agatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
       61 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
      121 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
      181 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
      241 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
      301 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
      361 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
      421 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
      481 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
      541 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
      601 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
      661 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      721 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      781 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
      841 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
      901 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
      961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2161 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     2221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2461 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     2521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cctcgagttg
     2881 taaaacgacg gccagtcaat tggaattcat ttaggtgact ctatagaagg gtccctatca
     2941 gtgatagaga gagctcgttg agttacctgc agcgattgta ctagttgcgg ccgcctgcgc
     3001 accacctgca ggacctcagc aatcgcttgg accagctttc ttaagggtac caataatttt
     3061 gtttaacttt aagaaggaga tatacatatg cgtaaaggcg aagaactgtt taccggtgtg
     3121 gttccgattc tggtggaact ggacggcgat gttaatggtc ataaattcag tgttcgcggc
     3181 gaaggtgaag gcgatgcgac gaacggcaaa ctgaccctga aatttatctg caccacgggt
     3241 aaactgccgg tcccgtggcc gacgctggtg accacgctga cctatggcgt tcaatgtttt
     3301 gcgcgttacc cggatcacat gaaacagcac gactttttca aatcggccat gccggaaggc
     3361 tatgtgcagg aacgtacgat tagctttaaa gacgatggta cgtataaaac ccgcgcggaa
     3421 gtgaaattcg aaggcgatac cctggttaac cgtatcgaac tgaaaggtat cgatttcaaa
     3481 gaagacggca atattctggg tcataaactg gaatataact tcaattccca caacgtgtac
     3541 atcaccgcgg ataaacagaa aaacggcatt aaagccaatt tcaaaatccg ccataatgtg
     3601 gaagatggta gcgttcagct ggccgaccac tatcagcaaa acacgccgat tggtgatggc
     3661 ccggtcctgc tgccggacaa tcactacctg agtacccagt ccgtgctgtc aaaagatccg
     3721 aacgaaaaac gtgaccacat ggtcctgctg gaatttgtga cggctgcggg tatcacccac
     3781 ggcatggacg aactgtataa aaggcctgct gctaacgatg aaaactacgc actggtggct
     3841 taataaaggc cttaataagg atccggcgcc gctcattcgc taatcgccac gacgcgtagt
     3901 cgacccgcca attcccatgg ggcgcgcctg ctgccaccgc tgagcaataa ctagcataac
     3961 cccttggggc ctctaaacgg gtcttgaggg gttttttgcc aggcatcaaa taaaacgaaa
     4021 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     4081 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg
     4141 gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac
     4201 ggatggcctt tttgcgtttc
//
