LOCUS       40924_18226        6819 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Acinetobacter baumannii cloning vector pET-RA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Aranda J, Poza M, Gomez B, Rumbo S, Rumbo C, Parreira JR, Rodriguez 
            P, Bou G.
  TITLE     A rapid and simple method for constructing stable mutants of 
            Acinetobacter baumannii
  JOURNAL   BMC Microbiol. 10 (1), 279 (2010)
  PUBMED    21062436
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Poza M, Aranda J, Bou G.
  TITLE     pET-RA cloning vector for Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6819)
  AUTHORS   Poza M, Aranda J, Bou G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2010) Microbiology, University Hospital A Coruna, 
            Spain, As Xubias, A Coruna, A Coruna 15006, Spain
REFERENCE   4  (bases 1 to 6819)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2010"; volume: "10"; issue: "1"; pages: "279"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (11-MAY-2010) Microbiology, University Hospital A Coruna, Spain, As 
            Xubias, A Coruna, A Coruna 15006, Spain"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             52..765
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      860..2197
     terminator      2406..2492
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2584..2611
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2630..2721
                     /label=AmpR promoter
     promoter        2732..2836
                     /label=AmpR promoter
     CDS             3137..3628
                     /codon_start=1
                     /gene="arr-2"
                     /product="rifampin ADP-ribosylating transferase ARR-2"
                     /label=arr-2
                     /note="confers rifampin resistance"
                     /protein_id="ADH43300.1"
                     /translation="MLCSQIPTIKGLKMVKDWIPISHDNYKQVQGPFYHGTKANLAIGD
                     LLTTGFISHFEDGRILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDD
                     PNLTNKKFPGNPTQSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED
                     "
     rep_origin      3847..4435
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4621..4761)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4866..5054)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 aaaaggatcc ggatccaaaa gatcctctag atttaagaag gagatataca tatgagtaaa
       61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      241 ttcgcgtatg gtcttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      481 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      541 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      601 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      661 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      721 gtaacagctg ctgggattac acatggcatg gatgaactat acaaagaatc tctgaaaatc
      781 tctcaggctg ttcacgctgc tcacgctgaa atcaacgaag ctggtcgtga agtagtaggt
      841 taactgcagc caagcttccg gattttaaca ttttgcgttg ttccaaaagt tatcaacagc
      901 ctagaacgtc ataggaagcg attacagaca ctttatagct atcagcatgg gaacataagg
      961 gcaggatgaa atatgggtct taaaacgcaa atggtgaggt tttagaggta ttttttgaag
     1021 atgattaagg cggttngttt ttaaaatttt tggcggctct caggctgctt acattttaac
     1081 cagttcagtg aaaagttctt tttcagcaaa tttctgttta gcaccatagc taaaacttgc
     1141 gtggaacata ttaagaattg accgaaatga cacaatctca attatatttt ttttgaaaag
     1201 ttttctttat caaatatttt aaatcattga tttatatata agtatacatt cattttaata
     1261 attaatcttt atttaacaat gatttatcta tattcaattg tttaattatt cttactaata
     1321 ttatctctat atcaatattt tttatttaaa aacatatgtt tagtagtgct tttgattaaa
     1381 gtaccagagg gagggagcag agctgaatgg gaaatactca ccctagagcg attcttaaaa
     1441 atcaccctaa agtattccca ttcgatgtac cgtcggtcgg tcgctttcgc atcagggatg
     1501 acatcactgt atcaagctgc cactgttatg attacgattg atagcaccgc ctgaacacgc
     1561 tcataaccgc caattaaatg actactcatt gcgtccgcta ctctgttcag ttcccctagt
     1621 aatagcgttt ttccgatgtg tgctagcgtc actgtacctc atcacccaca catggacaga
     1681 ttgagttaca gacattggct aaatttttgg ggtctatgct tgacaaagcg agttaaaccc
     1741 ttagaattta agncagntnc attaagcccc tgtggtgaaa tcattagcgg tcgtcaaacc
     1801 ttaatgcttt ngattgccga tatgtcagta tcgaagatct gtaccccata aattacggta
     1861 aagccccaag caattgcaag gggcttttat cttttttaac aaaaaaaaat ttataaatca
     1921 ggattttata ncactaaata atccaaagta catgagtaag tcatgaccac tcntgcgatg
     1981 tgtgtagcac tctgagtatc cgtattatat cagtgatgtc atatacaacc acataatgcg
     2041 gatgtatcac taactcccta gtatctttct gtctgcctgt gcgccccatc agtcgattat
     2101 caacaagtaa atctgtcttt tcttcaatta aatcatcaat ttcaatagct gctatagggt
     2161 tgcgttcttc aagataatca tagatatttc tacgatcgga agcttctgtt ttggcggatg
     2221 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     2281 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     2341 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2461 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     2521 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     2581 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     2641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2701 taatattgaa aaaggaagag taattcaggt gcgcggaacc cctatttgtt tatttttcta
     2761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2821 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2881 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2941 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3001 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3061 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3121 ttctcagaat gacttggtgt tatgcagcca aatcccaaca attaagggtc ttaaaatggt
     3181 aaaagattgg attcccatct ctcatgataa ttacaagcag gtgcaaggac cgttctatca
     3241 tggaaccaaa gccaatttgg cgattggtga cttgctaacc acagggttca tctctcattt
     3301 cgaggacggt cgtattctta agcacatcta cttttcagcc ttgatggagc cagcagtttg
     3361 gggagctgaa cttgctatgt cactgtctgg cctcgagggt cgcggctaca tatacatagt
     3421 tgagccaaca ggaccgttcg aagacgatcc gaatcttacg aacaaaaaat ttcccggtaa
     3481 tccaacacag tcctatagaa cctgcgaacc cttgagaatt gttggcgttg ttgaagactg
     3541 ggaggggcat cctgttgaat taataagggg aatgttggat tcgttagagg acttaaagcg
     3601 ccgtggttta cacgtcattg aagactagcc tggacagatc gctgagatag gtgcctcact
     3661 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3961 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4441 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4501 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4561 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4621 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     4681 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     4741 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4801 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4861 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     4921 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     4981 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     5041 tcctgtttgg tcacttgatg cctccgtgta agggggaatt tctgttcatg ggggtaatga
     5101 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     5161 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     5221 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     5281 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     5341 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     5401 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     5461 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     5521 gtggccagga cccaacgctg cctcagagtg ctacacacat cgcangagtg gtcatgactt
     5581 actcatgtac tttggattat ttagtgntat aaaatcctga tttataaatt tttttttgtt
     5641 aaaaaagata aaagcccctt gcaattgctt ggggctttac cgtaatttat ggggtacaga
     5701 tcttcgatac tgacatatcg gcaatcnaaa gcattaaggt ttgacgaccg ctaatgattt
     5761 caccacaggc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     5821 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     5881 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     5941 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     6001 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     6061 tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     6121 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     6181 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     6241 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     6301 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     6361 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     6421 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     6481 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     6541 cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     6601 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     6661 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     6721 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     6781 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagag
//
