LOCUS       Exported                4338 bp DNA     circular SYN 16-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(734..4338,1..733)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        103..121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             153..175
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             183..215
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      283..330
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        696..724
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(737..1552)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1674..2262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2448..2587
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2689..2880)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
       61 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
      121 ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa ggagatatac
      181 atatggctag catgactggt ggacagcaaa tgggtcgcgg atccggctgc taacaaagcc
      241 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      301 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
      361 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
      421 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
      481 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
      541 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      601 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      661 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
      721 tcaaacatga gaattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      781 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      841 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      901 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      961 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     1021 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1081 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     1141 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     1201 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     1261 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     1321 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     1381 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     1441 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     1501 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
     1561 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt
     1621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1801 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2341 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2401 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     2461 tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct
     2521 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     2581 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2641 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2701 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     2761 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     2821 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     2881 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     2941 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     3001 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     3061 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     3121 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     3181 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     3241 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     3301 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac
     3361 gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     3421 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     3481 agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc
     3541 catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat
     3601 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
     3661 atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     3721 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     3781 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     3841 cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     3901 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     3961 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     4021 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     4081 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     4141 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     4201 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     4261 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     4321 gcccgatctt ccccatcg
//
