LOCUS       40924_18121        4130 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(12..44)
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     RBS             complement(52..74)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(106..124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1685..1873
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1978..2117
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2303..2891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3065..3922)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3923..4027)
                     /label=AmpR promoter
ORIGIN
        1 ttcggatccc gacccatttg ctgtccacca gtcatgctag ccatatgtat atctccttct
       61 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
      121 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      181 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      241 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
      301 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
      361 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
      421 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
      481 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
      541 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
      601 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
      661 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
      721 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
      781 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
      841 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
      901 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
      961 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     1021 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     1081 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     1141 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     1201 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     1261 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     1321 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     1381 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     1441 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     1501 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     1561 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     1621 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
     1681 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     1741 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     1801 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     1861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     1981 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     2041 gagcagattg tactgagagt gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga
     2101 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3361 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3661 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//
