LOCUS       62056_9540        9515 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(5..144)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(249..437)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    complement(1212..1233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1249..2328)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(2329..2406)
                     /label=lacI promoter
     promoter        2513..2531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2594..2641
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    2874..2898
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2942..7045
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7094..7111
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     rep_origin      7262..7717
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(7812..8624)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      8746..9334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
      301 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
      361 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      421 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
      481 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      541 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      601 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      661 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
      721 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
      781 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
      841 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
      901 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      961 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     1021 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     1081 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     1141 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     1201 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     1261 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1321 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1381 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1441 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1501 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     1561 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1621 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1681 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1741 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1801 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1861 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1921 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1981 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     2041 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     2101 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     2161 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     2221 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     2281 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2341 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2401 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2461 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgccaa attaatacga
     2521 ctcactatag gaaccattca aaacgacata gcaagttaaa ataaggctag tccgttatca
     2581 acttgaaaaa gtgctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     2641 ggcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     2701 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     2761 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     2821 ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg
     2881 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     2941 catggacaag aagtactcca ttgggctcga tatcggcaca aacagcgtcg gctgggccgt
     3001 cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg
     3061 ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg agacggccga
     3121 agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga atcggatctg
     3181 ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag
     3241 gctggaggag tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg
     3301 caatatcgtg gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa
     3361 gaagcttgta gacagtactg ataaggctga cttgcggttg atctatctcg cgctggcgca
     3421 tatgatcaaa tttcggggac acttcctcat cgagggggac ctgaacccag acaacagcga
     3481 tgtcgacaaa ctctttatcc aactggttca gacttacaat cagcttttcg aagagaaccc
     3541 gatcaacgca tccggagttg acgccaaagc aatcctgagc gctaggctgt ccaaatcccg
     3601 gcggctcgaa aacctcatcg cacagctccc tggggagaag aagaacggcc tgtttggtaa
     3661 tcttatcgcc ctgtcactcg ggctgacccc caactttaaa tctaacttcg acctggccga
     3721 agatgccaag cttcaactga gcaaagacac ctacgatgat gatctcgaca atctgctggc
     3781 ccagatcggc gaccagtacg cagacctttt tttggcggca aagaacctgt cagacgccat
     3841 tctgctgagt gatattctgc gagtgaacac ggagatcacc aaagctccgc tgagcgctag
     3901 tatgatcaag cgctatgatg agcaccacca agacttgact ttgctgaagg cccttgtcag
     3961 acagcaactg cctgagaagt acaaggaaat tttcttcgat cagtctaaaa atggctacgc
     4021 cggatacatt gacggcggag caagccagga ggaattttac aaatttatta agcccatctt
     4081 ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt aacagagaag atctgttgcg
     4141 caaacagcgc actttcgaca atggaagcat cccccaccag attcacctgg gcgaactgca
     4201 cgctatcctc aggcggcaag aggatttcta cccctttttg aaagataaca gggaaaagat
     4261 tgagaaaatc ctcacatttc ggatacccta ctatgtaggc cccctcgccc ggggaaattc
     4321 cagattcgcg tggatgactc gcaaatcaga agagaccatc actccctgga acttcgagga
     4381 agtcgtggat aagggggcct ctgcccagtc cttcatcgaa aggatgacta actttgataa
     4441 aaatctgcct aacgaaaagg tgcttcctaa acactctctg ctgtacgagt acttcacagt
     4501 ttataacgag ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc cagcattcct
     4561 gtctggagag cagaagaaag ctatcgtgga cctcctcttc aagacgaacc ggaaagttac
     4621 cgtgaaacag ctcaaagaag actatttcaa aaagattgaa tgtttcgact ctgttgaaat
     4681 cagcggagtg gaggatcgct tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat
     4741 cattaaagac aaggacttcc tggacaatga ggagaacgag gacattcttg aggacattgt
     4801 cctcaccctt acgttgtttg aagataggga gatgattgaa gaacgcttga aaacttacgc
     4861 tcatctcttc gacgacaaag tcatgaaaca gctcaagagg cgccgatata caggatgggg
     4921 gcggctgtca agaaaactga tcaatgggat ccgagacaag cagagtggaa agacaatcct
     4981 ggattttctt aagtccgatg gatttgccaa ccggaacttc atgcagttga tccatgatga
     5041 ctctctcacc tttaaggagg acatccagaa agcacaagtt tctggccagg gggacagtct
     5101 tcacgagcac atcgctaatc ttgcaggtag cccagctatc aaaaagggaa tactgcagac
     5161 cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg cataagcccg agaatatcgt
     5221 tatcgagatg gcccgagaga accaaactac ccagaaggga cagaagaaca gtagggaaag
     5281 gatgaagagg attgaagagg gtataaaaga actggggtcc caaatcctta aggaacaccc
     5341 agttgaaaac acccagcttc agaatgagaa gctctacctg tactacctgc agaacggcag
     5401 ggacatgtac gtggatcagg aactggacat caatcggctc tccgactacg acgtggatca
     5461 tatcgtgccc cagtcttttc tcaaagatga ttctattgat aataaagtgt tgacaagatc
     5521 cgataaaaat agagggaaga gtgataacgt cccctcagaa gaagttgtca agaaaatgaa
     5581 aaattattgg cggcagctgc tgaacgccaa actgatcaca caacggaagt tcgataatct
     5641 gactaaggct gaacgaggtg gcctgtctga gttggataaa gccggcttca tcaaaaggca
     5701 gcttgttgag acacgccaga tcaccaagca cgtggcccaa attctcgatt cacgcatgaa
     5761 caccaagtac gatgaaaatg acaaactgat tcgagaggtg aaagttatta ctctgaagtc
     5821 taagctggtc tcagatttca gaaaggactt tcagttttat aaggtgagag agatcaacaa
     5881 ttaccaccat gcgcatgatg cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa
     5941 atatcccaag cttgaatctg aatttgttta cggagactat aaagtgtacg atgttaggaa
     6001 aatgatcgca aagtctgagc aggaaatagg caaggccacc gctaagtact tcttttacag
     6061 caatattatg aattttttca agaccgagat tacactggcc aatggagaga ttcggaagcg
     6121 accacttatc gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg gtagggattt
     6181 cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt
     6241 acagaccgga ggcttctcca aggaaagtat cctcccgaaa aggaacagcg acaagctgat
     6301 cgcacgcaaa aaagattggg accccaagaa atacggcgga ttcgattctc ctacagtcgc
     6361 ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt
     6421 caaggaactg ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa accccatcga
     6481 ctttctcgag gcgaaaggat ataaagaggt caaaaaagac ctcatcatta agcttcccaa
     6541 gtactctctc tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct
     6601 gcagaaaggt aacgagctgg cactgccctc taaatacgtt aatttcttgt atctggccag
     6661 ccactatgaa aagctcaaag ggtctcccga agataatgag cagaagcagc tgttcgtgga
     6721 acaacacaaa cactaccttg atgagatcat cgagcaaata agcgaattct ccaaaagagt
     6781 gatcctcgcc gacgctaacc tcgataaggt gctttctgct tacaataagc acagggataa
     6841 gcccatcagg gagcaggcag aaaacattat ccacttgttt actctgacca acttgggcgc
     6901 gcctgcagcc ttcaagtact tcgacaccac catagacaga aagcggtaca cctctacaaa
     6961 ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat
     7021 cgacctctct cagctcggtg gagacagcag ggctgacccc aagaagaaga ggaaggtggc
     7081 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
     7141 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     7201 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
     7261 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7381 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7441 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7501 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7561 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7621 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7681 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7801 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     7861 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     7921 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     7981 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     8041 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     8101 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     8161 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     8221 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     8281 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     8341 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     8401 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     8461 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     8521 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     8581 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     8641 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     8701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8881 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9481 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
