LOCUS       pET-23c(+).        3664 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of N-terminally T7-tagged proteins. 
            For other reading frames, use pET-23a(+) or pET-23b(+).
ACCESSION   .
VERSION     .
KEYWORDS    pET-23c(+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    158..203
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(205..237)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     RBS             complement(245..267)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(299..317)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             826..1014
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1119..1261
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1447..2035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2209..3066)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3067..3171)
                     /label=AmpR promoter
     rep_origin      complement(3198..3653)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
      241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
      301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
      361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
      421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
      481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
      541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
      601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
      661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
      721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
      781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
      841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
      901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
      961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     3661 gcca
//
