LOCUS       40924_17651        4674 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(404..451)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     RBS             complement(589..611)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(643..661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2226..2414
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2519..2661
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2847..3435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3609..4466)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4467..4571)
                     /label=AmpR promoter
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatcccgtcg acgcaaaaga gctgatcgcg
      541 ataggggttg tcaggactat tcctaggagt ttcgcccgca tatgtatatc tccttcttaa
      601 agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt
      661 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
      721 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
      781 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
      841 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
      901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
      961 gatcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     1021 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     1081 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     1141 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1201 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1261 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1321 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1381 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1441 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1501 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1561 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     1621 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     1681 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     1741 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     1801 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     1861 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     1921 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
     1981 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     2041 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
     2101 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
     2161 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
     2221 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     2281 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     2341 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     2401 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2461 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     2521 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     2581 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta
     2641 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3901 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4201 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     4261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4621 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//
