LOCUS       Exported                5671 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        257..275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    276..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             344..376
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      442..489
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(664..692)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        805..909
                     /gene="bla"
                     /label=AmpR promoter
     CDS             910..1770
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1941..2529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2715..2854
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2956..3147)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4459..5541)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(5542..5619)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
       61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      301 cctctagaaa taattttgtt taactttaag aaggagatat accatggcta gcatgactgg
      361 tggacagcaa atgggtcgga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      481 ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg
      541 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      601 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      661 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
      721 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      781 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      841 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      901 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      961 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1021 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1081 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1141 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1201 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1261 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1321 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1381 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1441 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1501 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1561 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1621 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1681 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1741 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2701 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2761 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2821 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2881 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2941 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     3001 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     3061 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3121 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3181 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3241 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     3301 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     3361 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     3421 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     3481 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     3541 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     3601 catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga
     3661 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     3721 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
     3781 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     3841 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     3901 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc
     3961 gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     4021 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     4081 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg
     4141 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     4201 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     4261 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     4321 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     4381 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4501 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     4561 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     4621 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     4681 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     4741 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     4801 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     4861 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     4921 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     4981 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     5041 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     5101 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     5161 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     5221 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     5281 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     5341 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     5401 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     5461 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     5521 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     5581 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     5641 tatgcgactc ctgcattagg aagcagccca g
//
