LOCUS       40924_17626       13791 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pESPBAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Huang H, Shang G.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2008) College of Life Sciences, No.1 Wenyuan Rd., 
            XiXia District, Nanjing, Jiangsu Province 210046, P.R.China
REFERENCE   2  (bases 1 to 13791)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (12-MAY-2008) College of Life Sciences, No.1 Wenyuan Rd., XiXia 
            District, Nanjing, Jiangsu Province 210046, P.R.China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     289..305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        312..330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(390..406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(438..468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    483..504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(792..1380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1554..2411)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2412..2516)
                     /label=AmpR promoter
     primer_bind     2990..3006
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3082..3100)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3118..3134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3142..3158)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3166..3196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3211..3232)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            complement(3447..3556)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3794..3805)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     protein_bind    3876..3975
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             3985..5799
                     /codon_start=1
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
                     /translation="VDTYAGAYDRQSRERENLSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5896..6696
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(7062..7706)
                     /codon_start=1
                     /product="catabolite gene activator"
                     /label=catabolite gene activator
                     /note="Crp"
                     /protein_id="ACD75468.1"
                     /translation="MVASALPAKIKNIDKLLVHCQRRRYTAKSNIICAGDRAETLSFII
                     KGSVTILIEDDDGHEMIIAYLNHGDFFGELGLFEPVDGEQQRSAWVRAKTECEVAEISY
                     EKFRELARQDPEILYALGSQMAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPDAMT
                     HPDGMQIKITRQEIGRIVGCSREMVGRVLKDLEERSLVQVKGKTMVVHGTR"
     promoter        complement(7706..7808)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      8735..8958
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             9049..9801
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    9807..10057
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             10383..11555
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             11558..12526
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    12602..13075
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(13335..13733)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(13751..13784)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
      181 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      241 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      301 ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cggggatccc
      361 cgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2581 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     2641 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2701 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     2761 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     2821 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     2881 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     2941 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     3001 gccagtgcca agcttgcatg cctgcaggtc gactctagag gatcctctag agtcgacctg
     3061 caggcatgca agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc
     3121 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3181 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3241 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3301 ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa ttctcatgtt tgacagctta
     3361 tcatcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg
     3421 tttaagggca ccaataactg ccttaaccgg ccagcctcgc agagcaggat tcccgttgag
     3481 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
     3541 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
     3601 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
     3661 gaccccgaag cagggttatg cagcggaaaa gatccgtcga cctgcaggca tgcaagctct
     3721 agcgattcca gacgtcccga aggcgtggcg cggcttcccc gtgccggagc aatcgccctg
     3781 ggtgggttac acgacgcccc tctatggccc gtactgacgg acacaccgaa gccccggcgg
     3841 caaccctcag cggatgcccc ggggcttcac gttttcccag gtcagaagcg gttttcggga
     3901 gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag ggtcgccgac
     3961 atgacacaag gggttgtgac cggggtggac acgtacgcgg gtgcttacga ccgtcagtcg
     4021 cgcgagcgcg agaatttgag cgcagcaagc ccagcgacac agcgtagcgc caacgaagac
     4081 aaggcggccg accttcagcg cgaagtcgag cgcgacgggg gccggttcag gttcgtcggg
     4141 catttcagcg aagcgccggg cacgtcggcg ttcgggacgg cggagcgccc ggagttcgaa
     4201 cgcatcctga acgaatgccg cgccgggcgg ctcaacatga tcattgtcta tgacgtgtcg
     4261 cgcttctcgc gcctgaaggt catggacgcg attccgattg tctcggaatt gctcgccctg
     4321 ggcgtgacga ttgtttccac tcaggaaggc gtcttccggc agggaaacgt catggacctg
     4381 attcacctga ttatgcggct cgacgcgtcg cacaaagaat cttcgctgaa gtcggcgaag
     4441 attctcgaca cgaagaacct tcagcgcgaa ttgggcgggt acgtcggcgg gaaggcgcct
     4501 tacggcttcg agcttgtttc ggagacgaag gagatcacgc gcaacggccg aatggtcaat
     4561 gtcgtcatca acaagcttgc gcactcgacc actcccctta ccggaccctt cgagttcgag
     4621 cccgacgtaa tccggtggtg gtggcgtgag atcaagacgc acaaacacct tcccttcaag
     4681 ccgggcagtc aagccgccat tcacccgggc agcatcacgg ggctttgtaa gcgcatggac
     4741 gctgacgccg tgccgacccg gggcgagacg attgggaaga agaccgcttc aagcgcctgg
     4801 gacccggcaa ccgttatgcg aatccttcgg gacccgcgta ttgcgggctt cgccgctgag
     4861 gtgatctaca agaagaagcc ggacggcacg ccgaccacga agattgaggg ttaccgcatt
     4921 cagcgcgacc cgatcacgct ccggccggtc gagcttgatt gcggaccgat catcgagccc
     4981 gctgagtggt atgagcttca ggcgtggttg gacggcaggg ggcgcggcaa ggggctttcc
     5041 cgggggcaag ccattctgtc cgccatggac aagctgtact gcgagtgtgg cgccgtcatg
     5101 acttcgaagc gcggggaaga atcgatcaag gactcttacc gctgccgtcg ccggaaggtg
     5161 gtcgacccgt ccgcacctgg gcagcacgaa ggcacgtgca acgtcagcat ggcggcactc
     5221 gacaagttcg ttgcggaacg catcttcaac aagatcaggc acgccgaagg cgacgaagag
     5281 acgttggcgc ttctgtggga agccgcccga cgcttcggca agctcactga ggcgcctgag
     5341 aagagcggcg aacgggcgaa ccttgttgcg gagcgcgccg acgccctgaa cgcccttgaa
     5401 gagctgtacg aagaccgcgc ggcaggcgcg tacgacggac ccgttggcag gaagcacttc
     5461 cggaagcaac aggcagcgct gacgctccgg cagcaagggg cggaagagcg gcttgccgaa
     5521 cttgaagccg ccgaagcccc gaagcttccc cttgaccaat ggttccccga agacgccgac
     5581 gctgacccga ccggccctaa gtcgtggtgg gggcgcgcgt cagtagacga caagcgcgtg
     5641 ttcgtcgggc tcttcgtaga caagatcgtt gtcacgaagt cgactacggg cagggggcag
     5701 ggaacgccca tcgagaagcg cgcttcgatc acgtgggcga agccgccgac cgacgacgac
     5761 gaagacgacg cccaggacgg cacggaagac gtagcggcgt agggttcatg tgcagctcca
     5821 tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg
     5881 tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga
     5941 aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg
     6001 gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag
     6061 gccctgcgtg ctgcgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg
     6121 gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct
     6181 gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca
     6241 gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc tcactcgcct
     6301 gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga
     6361 cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg
     6421 ccgagacact gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat
     6481 gaccactgct gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag
     6541 aaggaaggtc cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg
     6601 acagccctgg gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga
     6661 tgcgaagaat gcgatgccgc tcgccagtcg attggctgac tgcagtgtga tgtgctgcgc
     6721 gagtggggcg gcgtagcgtc agtcggggaa atcgacgggg ccttctgcga tggccgctac
     6781 aacgtcaacc tcaatggccg caaattggtg ggtaccgccc agcgctggcg tcagggcctg
     6841 ggtggcaagc gtccggtggt gctggtgcac ggtgcgctgc tgctggacaa cgagcgtgag
     6901 tcgatggtgg cggcggtcaa ccgcttcaac gagtgctgtg agctggagca acgctgtcgc
     6961 gccgatgcgc acatcgcctt gcacgaagtg gcgccggtag caccttggtt cgagcgcctt
     7021 tcgcaggcct acgccaaggt actggccgag ttgcccaagg actagcgggt accgtggacc
     7081 accatggtct tgcctttgac ctgcaccagg ctgcgttcct cgagatcctt gagaacgcgg
     7141 ccgaccattt cccgagagca accgacgatt cggccgattt cctgacgggt aatcttgatt
     7201 tgcatgccat cggggtgggt catggcgtcg ggctgcttgc acagttccag caggcaacgg
     7261 gcaactcgcc cggtcacgtc gaaaaatgcc aaatcgccaa ccttgcgcgt agtgttgcgc
     7321 aggcgctggg ccatctggct acccagggca tagaggattt ccgggtcctg gcgcgccagc
     7381 tcgcgaaact tctcgtagct gatttcggcc acttcgcatt ctgtcttggc gcgtacccag
     7441 gcactgcgct gctgctcgcc atcaacgggc tcgaacaggc ctagctcgcc aaagaaatcg
     7501 ccatggttga ggtaggcgat gatcatttca tggccgtcgt cgtcctcgat gaggatggtg
     7561 accgaaccct tgatgatgaa cgacagtgtc tcggcccggt cgccggcgca gatgatattg
     7621 cttttggcgg tgtagcggcg gcgctggcag tgtaccagca gcttgtcgat gttctttatc
     7681 ttggcgggta gggcggaggc aaccatttag cttccttagc tcctgaaaat ctcgataact
     7741 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     7801 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     7861 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcgcgataag
     7921 ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat ctgggaagtg
     7981 acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc tgctgacggt
     8041 gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc gatgcacatg
     8101 ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa gtccatcagt
     8161 tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca ccgggtgcag
     8221 tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg agaataaatg
     8281 ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt ctgttaaaca
     8341 gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat ctcccattat
     8401 cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga agtagtgttc
     8461 tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag gaacaataga
     8521 aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg acagaacaac
     8581 ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg ctcgccgcag
     8641 tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca ccagggaaca gcacttatat
     8701 attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact tatccacggg
     8761 gatattttta taattatttt ttttatagtt tttagatctt cttttttaga gcgccttgta
     8821 ggcctttatc catgctggtt ctagctagag aaggtgttgt gacaaattgc cctttcagtg
     8881 tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa ccctgtgaca
     8941 aattgccctc agaagaagct gttttttcac aaagttatcc ctgcttattg actctttttt
     9001 atttagtgtg acaatctaaa aacttgtcac acttcacatg gatctgtcat ggcggaaaca
     9061 gcggttatca atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc aaacgacctc
     9121 actgaggcgg catatagtct ctcccgggat caaaaacgta tgctgtatct gttcgttgac
     9181 cagatcagaa aatctgatgg caccctacag gaacatgacg gtatctgcga gatccatgtt
     9241 gctaaatatg ctgaaatatt cggattgacc tctgcggaag ccagtaagga tatacggcag
     9301 gcattgaaga gtttcgcggg gaaggaagtg gttttttatc gccctgaaga ggatgccggc
     9361 gatgaaaaag gctatgaatc ttttccttgg tttatcaaac gtgcgcacag tccatccaga
     9421 gggctttaca gtgtacatat caacccatat ctcattccct tctttatcgg gttacagaac
     9481 cggtttacgc agtttcggct tagtgaaaca aaagaaatca ccaatccgta tgccatgcgt
     9541 ttatacgaat ccctgtgtca gtatcgtaag ccggatggct caggcatcgt ctctctgaaa
     9601 atcgactgga tcatagagcg ttaccagctg cctcaaagtt accagcgtat gcctgacttc
     9661 cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc aatgcgcctc
     9721 tcatacattg agaaaaagaa aggccgccag acgactcata tcgtattttc cttccgcgat
     9781 atcacttcca tgacgacagg atagtctgag ggttatctgt cacagatttg agggtggttc
     9841 gtcacatttg ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt tccttcagcc
     9901 tgcatggatt ttctcatact ttttgaactg taatttttaa ggaagccaaa tttgagggca
     9961 gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg tgacctgata tcgggggtta
    10021 gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt ggctatccgc
    10081 gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg ctgagcgtaa
    10141 gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc tatgctcggt
    10201 tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct cttcttatct
    10261 ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga ctttgcgatt
    10321 ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc aatgattaaa
    10381 ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct ggtcatgaaa
    10441 tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg aggaaaataa
    10501 cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct caggctatca
    10561 gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga ggacgggttg
    10621 agcaacgtgt tggttataca attgaacaaa ttaatcatat gcgtgatgtg tttggtacgc
    10681 gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgcc cataaaggtg
    10741 gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg aaggggctac
    10801 gtgttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat cacggatggg
    10861 taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt ggggaaaagg
    10921 acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt attccttcct
    10981 gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa ggtaaactgc
    11041 ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat gactatgatg
    11101 tcatagttat tgacagcgcg cctaacctgg gtatcggcac gattaatgtc gtatgtgctg
    11161 ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc gcactgcagt
    11221 ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc gagcctgatg
    11281 tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg tggatggagg
    11341 agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt gaaacggatg
    11401 aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt gatcaacgct
    11461 cttcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc aatgaaattt
    11521 tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc ctgttattcc
    11581 aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac cagctgcccc
    11641 gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg ccattacttt
    11701 gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg atagtgttga
    11761 gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta ctgaggacgc
    11821 actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg cgttcggtcg
    11881 aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag ctgctgcact
    11941 taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga tggctgcatt
    12001 atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc agcgttatgc
    12061 aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg cggaaaatat
    12121 ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat cagttgttgc
    12181 tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc aaaaagcctt
    12241 tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc agaaaaaagc
    12301 tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc ttaaaacgtc
    12361 atctgcatca agaactagtt taagctcacg acatcagttt gctcctggag cgacagtatt
    12421 gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa ctgagtgtat
    12481 agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat gcgaccacgt
    12541 tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag aaagcataac
    12601 tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct gataatcaga
    12661 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
    12721 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg
    12781 ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
    12841 atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc
    12901 gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc tgattattag
    12961 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacgatccc
    13021 actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta ttgtcgatca
    13081 gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta ttgacatgtc
    13141 gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct gtggattgct
    13201 gctgtgtcct gcttatccac aacattttgc gcacggttat gtggacaaaa tacctggtta
    13261 cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgacg
    13321 agctcgcgag ctcggacatg aggttgcccc gtattcagtg tcgctgattt gtattgtctg
    13381 aagttgtttt tacgttaagt tgatgcagat caattaatac gatacctgcg tcataattga
    13441 ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga taatcattat
    13501 cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct cgcgggtttt
    13561 cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc ataacttaat
    13621 gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa agcgagcttt
    13681 ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg gaagtccgag
    13741 ctcatcgcta ataacttcgt atagcataca ttatacgaag ttatattcga t
//
