LOCUS       Exported                7746 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Cloning vector pESC-LEU, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Lu Q.
  TITLE     pESC-LEU
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7746)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7746)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(666..1757)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1758..2165)
                     /label=LEU2 promoter
     rep_origin      complement(2466..2921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3000..3165)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3313..3336)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        3383..4047
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     promoter        4058..4076
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4094..4123
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      4153..4342
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(4585..5173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5347..6204)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6205..6309)
                     /label=AmpR promoter
     rep_origin      complement(6336..7678)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
      241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
      301 attgagtgtt ttttatttgt tgtatttttt ttttttagag aaaatcctcc aatatataaa
      361 ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg tgccctcctc
      421 cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag ccattagtat
      481 caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc agccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtgga acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata tattgctgaa
     1801 atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac
     1861 aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat ttagtcatga
     1921 acgcttctct attctatatg aaaagccggt tccggcgctc tcacctttcc tttttctccc
     1981 aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc
     2041 cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa
     2101 aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg agtattccca
     2161 cagttaactg cggtcaagat atttcttgaa tcaggcgcct tagaccgctc ggccaaacaa
     2221 ccaattactt gttgagaaat agagtataat tatcctataa atataacgtt tttgaacaca
     2281 catgaacaag gaagtacagg acaattgatt ttgaagagaa tgtggatttt gatgtaattg
     2341 ttgggattcc atttttaata aggcaataat attaggtatg tagatatact agaagttctc
     2401 ctcgaccggt cgatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2461 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2521 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2581 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2641 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2701 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2761 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2821 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2881 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     2941 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgaattgg
     3001 agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
     3061 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
     3121 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc
     3181 aacaacgtat ctaccaacga tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt
     3241 agacaagccg acaaccttga ttggagactt gaccaaacct ctggcgaaga attgttaatt
     3301 aagagctcag atcttatcgt cgtcatcctt gtaatccatc gatactagtg cggccgccct
     3361 ttagtgaggg ttgaattcga attttcaaaa attcttactt tttttttgga tggacgcaaa
     3421 gaagtttaat aatcatatta catggcatta ccaccatata catatccata tacatatcca
     3481 tatctaatct tacttatatg ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa
     3541 aaccttctct ttggaacttt cagtaatacg cttaactgct cattgctata ttgaagtacg
     3601 gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct
     3661 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
     3721 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
     3781 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
     3841 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
     3901 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
     3961 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
     4021 tatactttaa cgtcaaggag aaaaaacccc ggatccgtaa tacgactcac tatagggccc
     4081 gggcgtcgac atggaacaga agttgatttc cgaagaagac ctcgagtaag cttggtaccg
     4141 cggctagcta agatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     4201 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     4261 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     4321 gaaggttttg ggacgctcga agatccagct gcattaatga atcggccaac gcgcggggag
     4381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6301 ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg
     6361 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     6421 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     6481 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     6541 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     6601 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     6661 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     6721 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     6781 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     6841 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     6901 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     6961 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     7021 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     7081 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     7141 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     7201 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     7261 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     7321 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     7381 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     7441 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     7501 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     7561 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     7621 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     7681 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7741 ttcgtc
//
