LOCUS       Exported                6706 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Cloning vector pESC-HIS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Lu Q.
  TITLE     pESC-HIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6706)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6706)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6706)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        317..503
                     /label=HIS3 promoter
     CDS             504..1163
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1426..1881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(1960..2125)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2273..2296)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        2343..3007
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     promoter        3018..3036
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3054..3083
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      3113..3302
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3545..4133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4307..5164)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5165..5269)
                     /label=AmpR promoter
     rep_origin      complement(5296..6638)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttc cacctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg
      841 gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc agagcggtgg
      901 tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag
      961 gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag gctagcagaa
     1021 ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt
     1081 tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc aacgatgttc
     1141 cctccaccaa aggtgttctt atgtagtgac accgattatt taaagctgca gcatacgata
     1201 tatatacatg tgtatatatg tatacctatg aatgtcagta agtatgtata cgaacagtat
     1261 gatactgaag atgacaaggt aatgcatcat tctatacgtg tcattctgaa cgaggcgcgc
     1321 tttccttttt tctttttgct ttttcttttt ttttctcttg aactcgacgg atctatgcgg
     1381 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     1441 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1501 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1561 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1621 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1681 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1741 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1801 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1861 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1921 atcggtgcgg gcctcttcgc tattacgcca gctgaattgg agcgacctca tgctatacct
     1981 gagaaagcaa cctgacctac aggaaagagt tactcaagaa taagaatttt cgttttaaaa
     2041 cctaagagtc actttaaaat ttgtatacac ttattttttt tataacttat ttaataataa
     2101 aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacga
     2161 tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg acaaccttga
     2221 ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctcag atcttatcgt
     2281 cgtcatcctt gtaatccatc gatactagtg cggccgccct ttagtgaggg ttgaattcga
     2341 attttcaaaa attcttactt tttttttgga tggacgcaaa gaagtttaat aatcatatta
     2401 catggcatta ccaccatata catatccata tacatatcca tatctaatct tacttatatg
     2461 ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa aaccttctct ttggaacttt
     2521 cagtaatacg cttaactgct cattgctata ttgaagtacg gattagaagc cgccgagcgg
     2581 gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc
     2641 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta
     2701 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg
     2761 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg
     2821 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatgcaaaaa
     2881 ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa
     2941 atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag
     3001 aaaaaacccc ggatccgtaa tacgactcac tatagggccc gggcgtcgac atggaacaga
     3061 agttgatttc cgaagaagac ctcgagtaag cttggtaccg cggctagcta agatccgctc
     3121 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     3181 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     3241 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     3301 agatccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3361 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3421 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3481 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3601 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3721 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3961 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5281 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     5341 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     5401 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
     5461 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     5521 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
     5581 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
     5641 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
     5701 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
     5761 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
     5821 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
     5881 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
     5941 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
     6001 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
     6061 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
     6121 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
     6181 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
     6241 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
     6301 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
     6361 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
     6421 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
     6481 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
     6541 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
     6601 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
     6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
