LOCUS       V006535                 3684 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006535
VERSION     V006535
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S,
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS,
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3684)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3684)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3684)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    389..486
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             488..1381
                     /gene="OGN"
                     /label="Mimecan"
                     /note="Mimecan from Homo sapiens. Accession#: P20774"
     protein_bind    complement(1383..1482)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1500..1518)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1523..1539)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1970..2758
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2854..3442
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg aagactctgc agtctacact tctcctgtta ctgcttgtgc ctctgataaa
      541 gccagcacca ccaacccagc aggactcacg cattatctat gattatggaa cagataattt
      601 tgaagaatcc atatttagcc aagattatga ggataaatac ctggatggaa aaaatattaa
      661 ggaaaaagaa actgtgataa tacccaatga gaaaagtctt caattacaaa aagatgaggc
      721 aataacacca ttacctccca agaaagaaaa tgatgaaatg cccacgtgtc tgctgtgtgt
      781 ttgtttaagt ggctctgtat actgtgaaga agttgacatt gatgctgtac cacccttacc
      841 aaaggaatca gcctatcttt acgcacgatt caacaaaatt aaaaagctga ctgccaaaga
      901 ttttgcagac atacctaact taagaagact cgattttaca ggaaatttga tagaagatat
      961 agaagatggt actttttcaa aactttctct gttagaagaa ctttcacttg ctgaaaatca
     1021 actactaaaa cttccagttc ttcctcccaa gctcacttta tttaatgcaa aatacaacaa
     1081 aatcaagagt aggggaatca aagcaaatgc attcaaaaaa ctgaataacc tcaccttcct
     1141 ctacttggac cataatgccc tggaatccgt gcctcttaat ttaccagaaa gtctacgtgt
     1201 aattcatctt cagttcaaca acatagcttc aattacagat gacacattct gcaaggctaa
     1261 tgacaccagt tacatccggg accgcattga agagatacgc ctggagggca atccaatcgt
     1321 cctgggaaag catccaaaca gttttatttg cttaaaaaga ttaccgatag ggtcatactt
     1381 ttacccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1441 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1501 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1561 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     1621 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     1681 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1741 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1801 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1861 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1921 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1981 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2041 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2101 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2161 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2221 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2281 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2341 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2401 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2461 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2521 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2581 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2641 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2701 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2761 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3001 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3241 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3541 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3601 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3661 gccgattcat taatgcagct ggca
//
