LOCUS       V006536                 3669 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006536
VERSION     V006536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S,
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS,
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3669)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3669)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3669)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    389..486
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             509..1366
                     /gene="MPG"
                     /label="DNA-3-methyladenine glycosylase"
                     /note="DNA-3-methyladenine glycosylase from Homo sapiens.
                     Accession#: P29372"
     protein_bind    complement(1369..1467)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1485..1503)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1508..1524)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1955..2743
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2839..3427
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg cccgcgcgca gcggggccca gttttgccga cggatggggc aaaagaagca
      541 gcgaccagct agagcagggc agccacacag ctcgtccgac gcagcccagg cacctgcaga
      601 gcagccacac agctcgtccg atgcagccca ggcaccttgc cccagggagc gctgcttggg
      661 accgcccacc actccgggcc cataccgcag catctatttc tcaagcccaa agggccacct
      721 tacccgactg gggttggagt tcttcgacca gccggcagtc cccctggccc gggcatttct
      781 gggacaggtc ctagtccggc gacttcctaa tggcacagaa ctccgaggcc gcatcgtgga
      841 gaccgaggca tacctggggc cagaggatga agccgcccac tcaaggggtg gccggcagac
      901 cccccgcaac cgaggcatgt tcatgaagcc ggggaccctg tacgtgtaca tcatttacgg
      961 catgtacttc tgcatgaaca tctccagcca gggggacggg gcttgcgtct tgctgcgagc
     1021 actggagccc ctggaaggtc tggagaccat gcgtcagctt cgcagcaccc tccggaaagg
     1081 caccgccagc cgtgtcctca aggaccgcga gctctgcagt ggcccctcca agctgtgcca
     1141 ggccctggcc atcaacaaga gctttgacca gagggacctg gcacaggatg aagctgtatg
     1201 gctggagcgt ggtcccctgg agcccagtga gccggctgta gtggcagcag cccgggtggg
     1261 cgtcggccat gcaggggagt gggcccggaa acccctccgc ttctatgtcc ggggcagccc
     1321 ctgggtcagt gtggtcgaca gagtggctga gcaggacaca caggcctgcc caactttctt
     1381 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1441 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     1501 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     1561 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     1621 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     1681 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     1741 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1801 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     1861 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     1921 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     1981 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2041 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2101 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2161 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2221 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2281 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2341 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     2401 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2461 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2521 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     2581 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     2641 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     2701 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     2761 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2941 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     3241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3481 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3541 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3601 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3661 cagctggca
//
