LOCUS       40924_17503        3666 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Human ORFeome Gateway entry vector pENTR223-ICK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li 
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley 
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, 
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, 
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
  PUBMED    15489335
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3666)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3666)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    389..486
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             488..1365
                     /codon_start=1
                     /note="unnamed protein product; hICK; incomplete
                     termination codon for C-terminal fusion"
                     /protein_id="SJX33038.1"
                     /translation="MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEEC
                     MNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRN
                     IMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTR
                     WYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKT
                     DWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQVFFH
                     FLVITFISNSE"
     protein_bind    complement(1365..1464)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1482..1500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1505..1521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1952..2740
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2836..3424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg aatagataca caacaatcag gcagctcggg gatggaacct acggttccgt
      541 cctgctggga agaagcattg agtctgggga gctgatcgct attaaaaaaa tgaaaagaaa
      601 attttattcc tgggaggaat gcatgaacct tcgggaggtt aagtctttaa agaagctcaa
      661 ccatgccaat gtagtcaaat taaaagaagt tatcagggaa aatgatcatc tttattttat
      721 cttcgagtac atgaaggaaa atctttacca gctcattaaa gagagaaata agttgtttcc
      781 tgagtctgct ataaggaata tcatgtatca gatattacaa ggactcgcat ttattcacaa
      841 acacggcttc tttcatcgag acttaaagcc tgagaacctc ctctgcatgg gaccagaact
      901 tgtgaaaatt gcagactttg gtttggcccg agaaatacga tcaaaacctc catatacaga
      961 ttatgtatct accagatggt acagggctcc agaagtactc ctgaggtcta ccaactacag
     1021 ctcccccatt gacgtctggg cggtgggctg catcatggca gaagtttaca ccctcaggcc
     1081 actcttccct ggagccagtg aaattgacac aatattcaaa atttgccaag tgctggggac
     1141 accaaaaaag actgactggc ctgaaggcta tcaactttca agtgcaatga acttccgttg
     1201 gccacagtgt gtacccaata acttaaagac cttgattccc aatgctagca gtgaagcagt
     1261 ccagctcctg agagacatgc ttcagtggga tcccaagaaa cgaccaacag ctagtcaggt
     1321 tttcttccat tttcttgtta taacgttcat atctaactct gaatacccaa ctttcttgta
     1381 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1441 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     1501 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     1561 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     1621 gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     1681 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     1741 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     1801 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     1861 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     1921 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
     1981 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     2041 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     2101 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     2161 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     2221 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     2281 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     2341 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     2401 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     2461 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     2521 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     2581 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     2641 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     2701 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     2761 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2941 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     3241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3661 ctggca
//
