LOCUS       V006540                 3675 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006540
VERSION     V006540
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S,
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS,
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3675)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3675)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    389..486
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             488..1372
                     /gene="HLF"
                     /label="Hepatic leukemia factor"
                     /note="Hepatic leukemia factor from Homo sapiens.
                     Accession#: Q16534"
     protein_bind    complement(1374..1473)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1491..1509)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1514..1530)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1961..2749
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2845..3433
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg gagaaaatgt cccgaccgct ccccctgaat cccaccttta tcccgcctcc
      541 ctacggcgtg ctcaggtccc tgctggagaa cccgctgaag ctcccccttc accacgaaga
      601 cgcatttagt aaagataaag acaaggaaaa gaagctggat gatgagagta acagcccgac
      661 ggtcccccag tcggcattcc tggggcctac cttatgggac aaaacccttc cctatgacgg
      721 agatactttc cagttggaat acatggacct ggaggagttt ttgtcagaaa atggcattcc
      781 ccccagccca tctcagcatg accacagccc tcaccctcct gggctgcagc cagcttcctc
      841 ggctgccccc tcggtcatgg acctcagcag ccgggcctct gcaccccttc accctggcat
      901 cccatctccg aactgtatgc agagccccat cagaccaggt cagctgttgc cagcaaaccg
      961 caatacacca agtcccattg atcctgacac catccaggtc ccagtgggtt atgagccaga
     1021 cccagcagat cttgcccttt ccagcatccc tggccaggaa atgtttgacc ctcgcaaacg
     1081 caagttctct gaggaagaac tgaagccaca gcccatgatc aagaaagctc gcaaagtctt
     1141 catccctgat gacctgaagg atgacaagta ctgggcaagg cgcagaaaga acaacatggc
     1201 agccaagcgc tcccgcgacg cccggaggct gaaagagaac cagatcgcca tccgggcctc
     1261 gttcctggag aaggagaact cggccctccg ccaggaggtg gctgacttga ggaaggagct
     1321 gggcaaatgc aagaacatac ttgccaagta tgaggccagg cacgggcccc tgtacccaac
     1381 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1441 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     1501 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1561 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     1621 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     1681 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     1741 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1801 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     1861 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1921 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1981 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2041 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     2101 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     2161 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2221 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2281 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     2341 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     2401 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     2461 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     2521 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     2581 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     2641 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     2701 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     2761 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     2821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     3001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     3241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3541 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3601 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3661 ttaatgcagc tggca
//
