LOCUS       40924_17493        3690 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Human ORFeome Gateway entry vector pENTR223-F12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li 
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley 
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, 
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, 
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
  PUBMED    15489335
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3690)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3690)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    389..486
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             488..1389
                     /codon_start=1
                     /note="unnamed protein product; hF12; incomplete
                     termination codon for C-terminal fusion"
                     /protein_id="SJX33376.1"
                     /translation="MPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCG
                     QRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPA
                     PEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALL
                     SPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDV
                     HGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKP
                     GVYTDVAYYLAWIREHTVS"
     protein_bind    complement(1390..1488)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1506..1524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1529..1545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1976..2764
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2860..3448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg cccgcgcagc cggcaccgcc gaagcctcag cccacgaccc ggaccccgcc
      541 tcagtcccag accccgggag ccttgccggc gaagcgggag cagccgcctt ccctgaccag
      601 gaacggccca ctgagctgcg ggcagcggct ccgcaagagt ctgtcttcga tgacccgcgt
      661 cgttggcggg ctggtggcgc tacgcggggc gcacccctac atcgccgcgc tgtactgggg
      721 ccacagtttc tgcgccggca gcctcatcgc cccctgctgg gtgctgacgg ccgctcactg
      781 cctgcaggac cggcccgcac ccgaggatct gacggtggtg ctcggccagg aacgccgtaa
      841 ccacagctgt gagccgtgcc agacgttggc cgtgcgctcc taccgcttgc acgaggcctt
      901 ctcgcccgtc agctaccagc acgacctggc tctgttgcgc cttcaggagg atgcggacgg
      961 cagctgcgcg ctcctgtcgc cttacgttca gccggtgtgc ctgccaagcg gcgccgcgcg
     1021 accctccgag accacgctct gccaggtggc cggctggggc caccagttcg agggggcgga
     1081 ggaatatgcc agcttcctgc aggaggcgca ggtaccgttc ctctccctgg agcgctgctc
     1141 agccccggac gtgcacggat cctccatcct ccccggcatg ctctgcgcag ggttcctcga
     1201 gggcggcacc gatgcgtgcc agggtgattc cggaggcccg ctggtgtgtg aggaccaagc
     1261 tgcagagcgc cggctcaccc tgcaaggcat catcagctgg ggatcgggct gtggtgaccg
     1321 caacaagcca ggcgtctaca ccgatgtggc ctactacctg gcctggatcc gggagcacac
     1381 cgtttcctgc ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1441 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1501 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1561 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     1621 gaccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     1681 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     1741 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1801 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     1861 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     1921 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     1981 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2041 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2101 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2161 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2221 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     2281 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     2341 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     2401 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2461 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2521 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2581 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     2641 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     2701 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     2761 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3001 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3241 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3481 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3541 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3601 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3661 gcgttggccg attcattaat gcagctggca
//
