LOCUS       40924_17478        3841 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    99..198
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             228..2036
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(2041..2140)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     rep_origin      complement(2259..2847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2993..3805)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
       61 cacgggccag agctgcagct agcatggatc tcgggcccca aataatgatt ttattttgac
      121 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
      181 tgtacaaaaa agcaggctca tttaacttta agaaggagat atataccatg gtccgtcctg
      241 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc
      301 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa
      361 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg
      421 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg
      481 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga
      541 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg
      601 ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc
      661 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct
      721 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg
      781 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc
      841 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg
      901 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg
      961 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct
     1021 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc
     1081 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggactta cgtggcaaag
     1141 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact
     1201 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg
     1261 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt
     1321 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc
     1381 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg
     1441 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt
     1501 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca
     1561 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc
     1621 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac
     1681 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat
     1741 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
     1801 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg
     1861 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg
     1921 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc
     1981 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaataat
     2041 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     2101 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct agcggtaata
     2161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
     2941 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     3001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     3061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     3121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     3181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     3241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     3301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     3361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     3421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     3481 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     3541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     3601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     3661 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     3721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag
     3781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     3841 g
//
