LOCUS       pENTR4.        3757 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) Dual Selection Vector for creating an entry vector by 
            restriction cloning. Same MCS as pENTR(TM)1A except with an NcoI 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create a Kozak sequence, clone a blunt fragment starting with 
            ATGG (where ATG is the start codon) into the XmnI site.
FEATURES             Location/Qualifiers
     source          1..3757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    461..510
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site, part 1"
     promoter        527..557
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             611..1288
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1611..1913
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_feature    1951..1982
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site, part 2"
     protein_bind    complement(1986..2085)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2208..3014
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gactggatcc ggtaccgaat tcgcggccgc attaggcacc ccaggcttta cactttatgc
      541 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
      601 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      661 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      721 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      781 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      841 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      901 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      961 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     1021 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     1081 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     1141 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1201 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggc
     1261 ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     1321 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     1381 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     1441 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     1501 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     1561 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     1621 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     1681 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     1741 ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     1801 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     1861 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     1921 caggctccct tatacacagc cagtctgcag gtcgacgaat tcgcggccgc actcgagata
     1981 tctagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2041 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc
     2101 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2161 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2221 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2281 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     2341 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     2401 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     2461 ttatccgtac tcctggtgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     2521 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     2581 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     2641 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     2701 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2761 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2821 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2881 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2941 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     3001 tcgatgagtt tttctaatca gaattggtta attggttgta acattattca gattgggccc
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
