LOCUS       pENTR3C.        3756 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) Dual Selection Vector for creating an entry vector by 
            restriction cloning. Other reading frames are available with 
            pENTR(TM)1A and pENTR(TM)2B.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR3C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create a Kozak sequence, clone a blunt fragment starting with 
            ATGG (where ATG is the start codon) into the XmnI site.
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    459..509
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site, part 1"
     RBS             463..468
                     /note="ribosome binding site"
     promoter        526..556
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             610..1287
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1610..1912
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_feature    1950..1981
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site, part 2"
     protein_bind    complement(1985..2084)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2207..3013
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3106..3694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
      481 actggatccg gtaccgaatt cgcggccgca ttaggcaccc caggctttac actttatgct
      541 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      601 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      661 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      721 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      781 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
      841 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      901 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      961 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1021 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     1081 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1141 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     1201 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg
     1261 gggcgtaatc tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     1321 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     1381 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     1441 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     1501 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     1561 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     1621 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     1681 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     1741 taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     1801 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     1861 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     1921 aggctccctt atacacagcc agtctgcagg tcgacgaatt cgcggccgca ctcgagatat
     1981 ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2041 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct
     2101 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2161 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2221 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2281 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2341 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2401 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2461 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2521 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2581 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2641 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2701 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2761 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2821 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2881 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     2941 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3001 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc
     3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3241 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3721 gttcctggcc ttttgctggc cttttgctca catgtt
//
