LOCUS       pENTR2B.        3755 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) Dual Selection Vector for creating an entry vector by 
            restriction cloning. Other reading frames are available with 
            pENTR(TM)1A and pENTR(TM)3C.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR2B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create a Kozak sequence, clone a blunt fragment starting with 
            ATGG (where ATG is the start codon) into the XmnI site.
FEATURES             Location/Qualifiers
     source          1..3755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    458..508
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site, part 1"
     promoter        525..555
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             609..1286
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1609..1911
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_feature    1949..1980
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site, part 2"
     protein_bind    complement(1984..2083)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2206..3012
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3105..3693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccgaattc gcggccgcat taggcacccc aggctttaca ctttatgctt
      541 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
      601 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      661 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      721 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      781 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
      841 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      901 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      961 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1021 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     1081 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1141 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1201 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg
     1261 ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     1321 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     1381 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     1441 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     1501 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     1561 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     1621 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     1681 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     1741 aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     1801 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     1861 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     1921 ggctccctta tacacagcca gtctgcaggt cgacgaattc gcggccgcac tcgagatatc
     1981 tagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2041 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc
     2101 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2161 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2221 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2281 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2341 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2401 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2461 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2521 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2581 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2641 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2701 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2761 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2821 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2881 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2941 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3001 gatgagtttt tctaatcaga attggttaat tggttgtaac attattcaga ttgggccccg
     3061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3721 ttcctggcct tttgctggcc ttttgctcac atgtt
//
