LOCUS       40924_17389        3174 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEMY07CD_Ttdh1_TER38, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Young EM, Zhao Z, Gielesen BE, Wu L, Benjamin Gordon D, Roubos JA, 
            Voigt CA.
  TITLE     Iterative algorithm-guided design of massive strain libraries, 
            applied to itaconic acid production in yeast
  JOURNAL   Metab. Eng. 48, 33-43 (2018)
  PUBMED    29753070
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Young E, Zhao Z, Gielesen B, Wu L, Gordon DB, Roubos J, Voigt C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) Genetics, DSM Biotechnology Center, 
            Alexander Fleminglaan 1, Delft, South Holland 2613AX, Netherlands
REFERENCE   3  (bases 1 to 3174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            48, 33-43 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2018) Genetics, DSM Biotechnology Center, Alexander 
            Fleminglaan 1, Delft, South Holland 2613AX, Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     790..806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2030..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2122..2979
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3106..3174
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
       61 gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact tcaagaactc
      121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta gggaactgcc
      721 aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc tacaaactct
      781 ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg ttctgataac
      841 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatggggg
      901 gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt tgcttgatat
      961 atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat aagatacgtt
     1021 gctttttcga ttgatgaaca cctataatta aactattcat ctattattta tgattttttg
     1081 tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg aaaataataa
     1141 agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca aaatataata
     1201 caaactataa gatgttatca gtatttatta tgcatttaga atacgtactc agcgtctggg
     1261 ttccccatcg gtgatgtcgt ataagagacg tataggagac ctatagtgtc ttctatagcg
     1321 cgcaggtgtg tcgcccttcg ctgaaggtct cgtaaagcaa tcttgatgag gataatgatt
     1381 tttttttgaa tatacataaa tactaccgtt tttctgctag attttgtgaa gacgtaaata
     1441 agtacatatt actttttaag ccaagacaag attaagcatt aactttaccc ttttctcttc
     1501 taagtttcaa tactagttat cactgtttaa aagttatggc gagaacgtcg gcggttaaaa
     1561 tatattaccc tgaacgtggt gaattgaagt tctaggatgg tttaaagatt tttccttttt
     1621 gggaaataag taaacaatat attgctgcct tcctcggaga cccgtcaaaa gggcgacacg
     1681 aagaccatat acgtctccta taggtctcgt ataacctgct tcctatacga catcaccgat
     1741 ggggaacacg aagcgatcca gcccccctaa ttagcccggg cgaaaggccc agtctttcga
     1801 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca
     1861 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
     1921 ccgcgctact gccgccaggc aaacaagggg tgttatgagc catattcagg tataaatggg
     1981 ctcgcgataa tgttcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2341 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2641 gacgagcgtg acaccacgat gcctgtagcg atggcaacaa cgttgcgcaa actattaact
     2701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatcc
     2821 ggagccggtg agcgtggttc tcgcggtatc atcgcagcgc tggggccaga tggtaagccc
     2881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2941 cagatcgctg agataggtgc ctcactgatt aagcattggt aaaagcagag cattacgctg
     3001 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc
     3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggt
//
