LOCUS       40924_17374       10195 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast expression vector pEMBLyex-kanMX4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10195)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(696..1209)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1697..1713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1980..3071)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(3429..4775)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    complement(4778..4834)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    5161..5278
                     /label=UAS
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             complement(5538..6338)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6339..6555)
                     /label=URA3 promoter
     primer_bind     complement(6575..6591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6599..6615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6623..6653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6668..6689)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6977..7565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7738..8595)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8596..8700)
                     /label=AmpR promoter
     gene            complement(8734..10090)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcatgcc tgcaggtcga
     4801 ctctagagga tccccgggta ccgagctcga attgatccgg taatttagtg tgtgtatttg
     4861 tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat gtataaggaa
     4921 agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc acatgcatgc
     4981 catatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag cactaaagtt
     5041 gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga ttagcctaaa
     5101 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
     5161 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
     5221 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     5281 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     5341 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
     5401 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatcgggt
     5461 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
     5521 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
     5581 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
     5641 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
     5701 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
     5761 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
     5821 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
     5881 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
     5941 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
     6001 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
     6061 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
     6121 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
     6181 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
     6241 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     6301 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttccggttt
     6361 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata
     6421 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga
     6481 ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg
     6541 atgaattgaa ttgaaaagct aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6661 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6721 cgggaaacct gtcgtgccan ctggattaat gaatcggcca acgcgcgggg agaggcggtt
     6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7621 gttagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8701 cacatttccc cgaaaagtgc cacctgacgt cagcagtata gcgaccagca ttcacatacg
     8761 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
     8821 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
     8881 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattag
     8941 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     9001 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     9061 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     9121 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     9181 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     9241 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     9301 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     9361 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     9421 tctaatacct ggaatgctgt tttgccgggg atcgcagtgg tgagtaacca tgcatcatca
     9481 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     9541 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     9601 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     9661 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     9721 gaaacgtgag tcttttcctt acccatggtt gtttatgttc ggatgtgatg tgagaactgt
     9781 atcctagcaa gattttaaaa ggaagtatat gaaagaagaa cctcagtggc aaatcctaac
     9841 cttttatatt tctctacagg ggcgcggcgt ggggacaatt caacgcgtct gtgaggggag
     9901 cgtttccctg ctcgcaggtc tgcagcgagg agccgtaatt tttgcttcgc gccgtgcggc
     9961 catcaaaatg tatggatgca aatgattata catggggatg tatgggctaa atgtacgggc
    10021 gacagtcaca tcatgcccct gagctgcgca cgtcaagact gtcaaggagg gtattctggg
    10081 cctccatgtc gctggccggg tgacccggcg gggacgaggc gacgtctaag aaaccattat
    10141 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa
//
