LOCUS       62056_9175        6022 bp DNA     circular SYN 14-APR-2019
DEFINITION  Cloning vector pEM021, complete sequence.
ACCESSION   MK431398
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Mancera E, Frazer C, Porman AM, Ruiz-Castro S, Johnson AD, Bennett 
            RJ.
  TITLE     Genetic Modification of Closely Related Candida Species
  JOURNAL   Front Microbiol 10, 357 (2019)
  PUBMED    30941104
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Mancera E, Frazer C, Porman AM, Ruiz S, Johnson AD, Bennett RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Departmento de Ingenieria Genetica, Centro 
            de Investigacion y de Estudios Avanzados del Instituto Politecnico 
            Nacional, Unidad Irapuato, Km. 9.6 Libramiento Norte Carrera 
            Irapuato-Leon, Irapuato, Guanajuato 36824, Mexico
REFERENCE   3  (bases 1 to 6022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 10, 357 (2019)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2019) Departmento de Ingenieria Genetica, Centro de 
            Investigacion y de Estudios Avanzados del Instituto Politecnico 
            Nacional, Unidad Irapuato, Km. 9.6 Libramiento Norte Carrera 
            Irapuato-Leon, Irapuato, Guanajuato 36824, Mexico"
FEATURES             Location/Qualifiers
     source          1..6022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    309..949
                     /label=promoter region from Candida albicans TEF2
                     /note="promoter region from Candida albicans TEF2"
     CDS             956..1978
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    1988..2375
                     /label=terminator region from Candida albicans ACT1
                     /note="terminator region from Candida albicans ACT1"
     promoter        complement(2456..2474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2481..2497)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2638..3066
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3410..4201
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4222..5079
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5253..5841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc cctttgccac
      301 ctgacgtcgt atagtgcttg ctgttcgata ttgctagagg caaagaaaaa ggattacctc
      361 acgcctaaaa tggaatttga aaattgagga gatgagttga gcttttgtct tttacacttt
      421 ccctattcaa ttgtgccgca aaaacttcaa tatctaacac agtgtgaaat atttgttatg
      481 tataccgaaa gccaaatacc atttaaactg gtatttcaag atttcataaa tcaagtttct
      541 ggttaaatta attgctgtta aacagaacaa gagcttcaca ttgatgttgg aataactact
      601 atagaattat aaaatacaca aaaccaggtc gtgtacgcaa taacttttca atgtatgaaa
      661 aaattttttt tttttttttg cttcttctct tcgcttcttt tttctagtta tcaatttggc
      721 aatgaattga tttgattgcc ctttgtttag tttactatga aaaaaaattt tttttttgtc
      781 tctgtcagat tttctctcat tttttttttt gcctcataat gaacaactca aaaaagaata
      841 taaatttctc atcaaagacc tttccaattg aaaatttttt ctttccttct tttccttttt
      901 ttttttcttt ctttcttctt ttctaagtat acattttata taatcaatcc tcgagatgaa
      961 aaaaccagaa ttgactgcta cttctgttga aaaatttttg attgaaaaat ttgattctgt
     1021 ttctgatttg atgcaattgt ctgaaggtga agaatcaaga gctttttctt ttgatgttgg
     1081 tggtagaggt tatgttttga gagttaattc ttgtgctgat ggtttttata aagatagata
     1141 tgtttataga cattttgctt ctgctgcttt gccaattcca gaagttttgg atattggtga
     1201 attttctgaa tctttgactt attgtatttc aagaagagct caaggtgtta ctttgcaaga
     1261 tttgccagaa actgaattgc cagctgtttt gcaaccagtt gctgaagcta tggatgctat
     1321 tgctgctgct gatttgtctc aaacttctgg ttttggtcca tttggtccac aaggtattgg
     1381 tcaatatact acttggagag attttatttg tgctattgct gatccacatg tttatcattg
     1441 gcaaactgtt atggatgata ctgtttctgc ttctgttgct caagcattgg atgaattgat
     1501 gttgtgggct gaagattgtc cagaagttag acatttggtt catgctgatt ttggttctaa
     1561 taatgttttg actgataatg gtagaattac tgctgttatt gattggtctg aagctatgtt
     1621 tggtgattct caatatgaag ttgctaatat ttttttttgg agaccatggt tggcttgtat
     1681 ggaacaacaa actagatatt ttgaaagaag acatccagaa ttggctggtt ctccaagatt
     1741 gagagcttat atgttgagaa ttggtttgga tcaattgtat caatctttgg ttgatggtaa
     1801 ttttgatgat gctgcttggg ctcaaggtag atgtgatgct attgttagat caggtgctgg
     1861 tactgttggt agaactcaaa ttgctagaag atcagctgct gtttggactg atggttgtgt
     1921 tgaagttttg gctgattctg gtaatagaag accatctact agaccaagag ctaaagaata
     1981 aggatccgag tgaaattctg gaaatctgga aatctggttt tgtattcttg ttattcttct
     2041 ttttgttatt acatatataa cttgttactt ttttaaaaaa atctttgttt attttataaa
     2101 tatataaaac taaatttaag aaaaagagaa aaatgtttta tttgagagat tgaaatttta
     2161 cttgaattta gcttagcttt tataaagtat tattatgtaa aaaaacaaaa caaatataca
     2221 ttaaaaagtt aagactataa aatagccacc caaggcattt ctatatcttg ttgttgttgt
     2281 tttcatcttc tgtatcagag gaacttattt tattattttc gtcacgggta ttttctcttg
     2341 tttgatgatt catcccattc attccatcat aaaatgacgt ctaagaaggg cgaattctgc
     2401 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat
     2461 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2521 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2581 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     2641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3061 cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc
     3121 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     3181 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     3241 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     3301 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     3361 gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     3421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     3601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3721 atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     3901 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     3961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4201 ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4441 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6001 tcagtgagcg aggaagcgga ag
//
