LOCUS       V015996                 5945 bp    DNA     circular SYN 14-APR-2019
DEFINITION  Exported.
ACCESSION   V015996
VERSION     V015996
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Mancera E, Frazer C, Porman AM, Ruiz-Castro S, Johnson AD, Bennett
            RJ.
  TITLE     Genetic Modification of Closely Related Candida Species
  JOURNAL   Front Microbiol 10, 357 (2019)
   PUBMED   30941104
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Mancera E, Frazer C, Porman AM, Ruiz S, Johnson AD, Bennett RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) Departmento de Ingenieria Genetica, Centro
            de Investigacion y de Estudios Avanzados del Instituto Politecnico
            Nacional, Unidad Irapuato, Km. 9.6 Libramiento Norte Carrera
            Irapuato-Leon, Irapuato, Guanajuato 36824, Mexico
REFERENCE   3  (bases 1 to 5945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 10, 357 (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-JAN-2019) Departmento de Ingenieria Genetica, Centro de
            Investigacion y de Estudios Avanzados del Instituto Politecnico
            Nacional, Unidad Irapuato, Km. 9.6 Libramiento Norte Carrera
            Irapuato-Leon, Irapuato, Guanajuato 36824, Mexico"
FEATURES             Location/Qualifiers
     source          1..5945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        239..257
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    337..1332
                     /gene="HIS1"
                     /label="contains promoter and 5' UTR"
                     /note="contains promoter and 5' UTR"
     CDS             1333..2226
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     misc_feature    2230..2762
                     /gene="HIS1"
                     /label="contains 3' UTR and terminator"
                     /note="contains 3' UTR and terminator"
     promoter        complement(2833..2851)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2858..2874)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3012..3311
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3663..4454
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             4664..5035
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     rep_origin      5176..5764
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggtg tcaaatgtgc cactgtatac
      361 gcattttcaa tcgaaaactt caagagactg cagtacgaag tcaaatggtt aatggagtta
      421 gccaaatcaa agtttacaca aataaaccag catggattgt tgtgtgagga gtatggagtt
      481 aaaatcagaa tattaggaaa tactaaactt ttaccaagag atgtattgga aattctcgaa
      541 aagacagagg aaataacgaa aaacaatact cgtgccgtgt tgaatgtttg cttcccatat
      601 acatcaagag acgaaataac ccatctgatc aagtcagtgg ttgatgaagc gaccaaaggt
      661 ggtattgaca taacagaaga ggctattgac aggcaccttt atacgaaaga tgtaccaccc
      721 ttggatttat tagtaagaac atcagggaca tttaggcttt ctgactttct tttatggcaa
      781 acagtgtcgc cagaatgtgc cgttgtgttt gtcgataaat tatggccggc attcaagcca
      841 tgggatatgc tcaaaatatt gcttaactgg gggtttaaca aatacatgta tggtaatcca
      901 aatgggtatg gggttagtag taatttggta gtgaaaaccc cggtagacgg agaattaata
      961 aataccccaa aatccaccac aggactcgac aggtacctgg aggatgagga gacagaagtt
     1021 agtagtaaca atttggggac agaagaggat acagtgacat cagaggaaga agacagcgaa
     1081 ggtatagtca aaaagcaata ggacagcaag acaatttatt tatttaatag ttttttataa
     1141 atgtaaaatc tatcacaaca tgtgtactta attttctgtg gtaggggagg ccatttcgtt
     1201 gaaacgtgtc gcgagcggtt gtgagaattg actcattttg attattacgc aaccattgaa
     1261 attttttatt tttttttggt gaagattttt cccacacaac ttcttctttt acttaaccac
     1321 caactaccga taatggattt agtcaatcat ttaccagacc gtttgttatt tgctgttcct
     1381 aaaaagggca gattatacga aaaatgctgt aacttattga gtggtgccga tatacagttt
     1441 agaagatcta atagattaga tatagcactt tctacaaact tgccaattgc attaatcttc
     1501 ttgcctgcag ctgatatccc agttttcgtt ggagaaggta attgtgactt gggtataact
     1561 gggttagacc aaatcaaaga agctgaacaa ttcgacaaca tcgaggactt gttggatttg
     1621 aaatttggtt catgcaaatt gcagatccaa gttccagcag atggcgagta cgaaaagcca
     1681 gaacagcttg ttggaaagaa aattgtgtct tcatttacaa aattgagtac cgactatttc
     1741 aaacaattgt cagacaaacc tactaatatc agatatgtcg gtggttccgt tgaggcttct
     1801 tgtgccttgg gtgttgctga tgctattgtc gatttggttg aaagtggtga aactatgaaa
     1861 gcagctgggt taaaggccat agaaaccata ttggagactt cggctcattt gatttcgtca
     1921 aagaaaagta aattcccaga aatggtcaat ataatcgtcc aaagacttca aggtgtttta
     1981 gctgcccagg aatatgtctt gtgtaactac aatgccccaa aatccatcca agcaaaatgt
     2041 ttaaccatta ctccaggcag aagggccgct actgtctcta ctttggacaa acacagcgac
     2101 gacgaagagg actgggttgc catctcatcc atggttaata gaaaggaaat cggtaatgta
     2161 atggacgaat tgaagaaagc tggtgcaacc gatatattgg tactcgagat atcaaattgt
     2221 agagtttaaa agaagtgata gtttctcata aatattatac atatatactt atagatctac
     2281 ttcaatttga ctcaatctgt cttataccat ctttatctac caccttgatg tacacacctt
     2341 taaagtcaat aggcattctc gtttgtaatt ccttgacaca catatccatt aactttaaac
     2401 catcttcgac cgtcatgtcc tttctataat gtttatctaa caaagatgtt gtgtaaaatg
     2461 cagcgtagcc atgagcacca taagggagtt caacttgtgt acccaaataa tctatccaat
     2521 tcaacgatgg ctcatttttc tttacatcat atcctccaat taaacagttc acctggtacg
     2581 gttttctaga tctaagggaa ctggccaatt ggtttctcac aaacgaggct gttgcctttg
     2641 gagataactc aatgtcattt tctctcatcg agtacaattg aatgtttgct tgaacatact
     2701 cggcaaactg aaccgtatca ccagcttcac cagtgtatgc tattaagttg tgtgcattta
     2761 aaaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
     2821 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     2881 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2941 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3001 tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     3061 tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca
     3121 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     3181 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     3241 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     3301 tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc tagatccttt
     3361 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     3421 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     3481 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3541 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
     3601 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     3661 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3721 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3781 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3841 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3901 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3961 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4021 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4081 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4141 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4201 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4261 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4321 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4381 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4441 ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta
     4501 cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa atgtgcgcgg
     4561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4621 accctgataa atgcttcaat aatagcacgt gaggagggcc accatggcca agttgaccag
     4681 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4741 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4801 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4861 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4921 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4981 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     5041 cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5101 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5161 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5221 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5281 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5341 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5401 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5461 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5521 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5581 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5641 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5701 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5761 gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca
     5821 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5881 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5941 ggaag
//
