LOCUS       V007578                 7022 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007578
VERSION     V007578
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Rawlinson EL, Nes IF, Skaugen M.
  TITLE     LasX, a transcriptional regulator of the lactocin S biosynthetic
            genes in Lactobacillus sakei L45, acts both as an activator and a
            repressor
  JOURNAL   Biochimie 84 (5-6), 559-567 (2002)
   PUBMED   12423800
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L.
  TITLE     Plasmid p256 from Lactobacillus plantarum represents a new type of
            replicon in lactic acid bacteria, and contains a
            toxin-antitoxin-like plasmid maintenance system
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)
   PUBMED   15699191
REFERENCE   3  (bases 1 to 7022)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   4  (bases 1 to 7022)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochimie
            84 (5-6), 559-567 (2002)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     77..93
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(260..994)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(1091..1174)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77677.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(1091..1174)
                     /gene="ermBL"
                     /label="ermBL"
     misc_feature    1315..2217
                     /note="p256 replication region; allows replication in
                     Lactobacillus species"
     oriT            complement(2469..2577)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        2754..2858
                     /label="AmpR promoter"
     CDS             2859..3716
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3966..4496
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      4718..4836
                     /note="Pneo; neomycin phosphotransferase promoter"
                     /regulatory_class="promoter"
     CDS             4888..6033
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     primer_bind     6465..6481
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6491..6509
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(6518..6625)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(6638..6656)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6677..6693)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6701..6717)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6725..6755)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6770..6791)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gatctgctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
       61 tcccagtcac gacgttgtaa aacgacggcc agtgaattaa ttcttgaaga cgaaaggcga
      121 accccgatac aaattccccg taggcgctag ggacctcttt agcttcttgg aagctgtcag
      181 tagtatatct aataatttat ctccattccc tttagtaacg tgtaactttc caaatttaaa
      241 aaagcgactc atagaattat ttcctcccgt taaataatag ataactatta aaaatagaca
      301 atacttgctc ataagtaatg gtacttaaat tgtttacttt ggcgtgtttc attgcttgac
      361 gaaactgatt tttagtaaac agttgacgat attctcgatt gacccatttt gaaacaaagt
      421 acttatatag cttccaatat ttatctggaa catctgtggt atggcgggta agttttatta
      481 agacactgtt tacttttggt ttaggatgaa agcattccgc tggcagctta agcaattgct
      541 gaatcgagac ttgagtgtgc aagagcaacc ctagtgttcg gtgaatatcc aaggtacgct
      601 tgtagaatcc ttcttcaaca gtcagataga tgtcagacgc acggctttca aaaaccactt
      661 ttttaataat ttgtgtgctt aaattgtaag gaatattccc aacaatttta tacctctgtt
      721 tgttagggaa ttgaaactgt agaatatctt ggtgaattaa agtgacacga gtattcagtt
      781 ttaatttttc tgacgataag ttgaatagat gactgtctaa ttcaatagac gttacctgtt
      841 tacttatttt agccagtttc gtcgttaaat gccctttacc tgttccaatt tcgtaaacgg
      901 tatcggtttc ttttaaattc aattgtttta ttatttggtt gagtactttt tcactcgtta
      961 aaaagttttg agaatatttt atatttttgt tcatgtaatc tctcctgaag ttaaacgatt
     1021 tgtttgtaat tttagttatc tgtttaaaaa gtcataagat tagtcactgg taggaattaa
     1081 tctaacgtat ttatttatct gcgtaatcac tgtttttagt ctgtttcaaa acagtagatg
     1141 ttttatctac attacgcatt tggaatacca acatgacgaa tccctccttc ttaattacaa
     1201 atttttagca tctaatttaa cttcaattcc tatttttagc atctaattta acttcaattc
     1261 ctattataca aaattttaag ataatgcact atcaacacac tcttaagttt gcttctaagg
     1321 ggtaccgaat tcctagtata ttaaataaca ccttaagtct agcaccaacc cgcacgcata
     1381 gcggtgctta aaccatcaag ggtcaagccc ttaggctctc tcaaacagtt atcctaatcg
     1441 tgaataactg cgcttctttt gcagtataaa gagagaactc tttatcagac aatttaagct
     1501 caaccagccc ttgcactaac tattattaga gttggtttta gcagcaaccc gaataatctg
     1561 cgttaatagt tagcctgtcc gtatcatttc ctagtcttcc agccacgtct ttagtcgcgt
     1621 tgatctcgac aaggtttagc atacctatgt tgttaactgc aagcggggtc acgaacgaca
     1681 ctcacgggag gttttactag ctaagaacag gtttccagcc tttagttgct ttgatggttg
     1741 ctaaccattg aataacaaaa aaacggttgc tatcaggttt ctgttaagat tcccgataac
     1801 aaccgtttac tttaagtatc aatggttgaa aaacttagcc tacatgttat aatagtacca
     1861 agttagatag cttgtattgg tagtacttgc tatcgaaaat cttatcaggt tgtgctgata
     1921 agtcgtgaat cctaactctg ctaagttgag ggttcttttt tttgcgttca tttattaagt
     1981 tgagtacatt ataaccgtaa tataagatta atacaacctt tatcatttta acgtctcaac
     2041 cagccgaata atcctttttt aagcgtttta gtttcttccc ccttgtaatg atccccagtg
     2101 ggttctaaaa cgtctgtatc gtcctttgag gcgtcctcgc tggtcttttg tggggcatcg
     2161 agtagttttt cattttgacg gttagtggat aagtttaatt gctgttgctg atcctctaat
     2221 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2281 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     2341 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2401 acaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2461 agagcttacg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     2521 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     2581 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     2641 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     2701 gcagcggaaa agatccgtcg atcgacccag gtggcacttt tcggggaaat gtgcgcggaa
     2761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     3121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg ggggatcagg accgctgccg gagcgcaacc cactcactac agcagagcca
     3961 tgtagggccg ccggcgttgt ggataccacg cggaaaactt ggccctcact gacagatgag
     4021 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
     4081 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
     4141 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
     4201 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
     4261 ggcagagtga tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca
     4321 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
     4381 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctggcgc
     4441 gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg
     4501 cggcacccca tccccccagg ggctgcgccc ctcggccgcg aacgacctca ccccaaaaat
     4561 ggcagccacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
     4621 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
     4681 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
     4741 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     4801 gccgccaagg atctgatggc gcaggggatc aagatcgacg gatcgatccg gggaattaat
     4861 tccggggcaa tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg
     4921 caagaactga tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc
     4981 gtcatgcgtg cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg
     5041 gccaagatcg agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc
     5101 gccgtggagc gttcgcgtcg tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc
     5161 atcgacacgc gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca
     5221 aaacaggtca gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag
     5281 gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca
     5341 aacgacacgg cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg
     5401 ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc
     5461 gagctgcggg ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc
     5521 acccctatcg gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg
     5581 tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg
     5641 gcgatgggct tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc
     5701 ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa
     5761 atcgtcgtgc tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag
     5821 ctgtcgccga cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg
     5881 ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg
     5941 cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc
     6001 tgggtcaatg atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct
     6061 gggggttcag cacctgcatg attattgaag catttatcag ggttattgtc tcatgagcgg
     6121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6181 aaaagtgcca cctgccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6301 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     6361 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6421 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     6481 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
     6541 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     6601 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     6661 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7021 tg
//
